Our group has generated web resources from our single-cell and bulk transcriptomic datasets, where you can enter your gene of interest and investigate its expression pattern in the identified oligodendrocyte lineage populations/states or how your genes of interest are differentially expressed.
Single nuclei RNA- sequencing from the white matter of individuals with progressive MS and non-neurological controls
This dataset accompanies the manuscript below, and features the CCA alignment clustering of white matter tissue from 4 progressive Multiple sclerosis patients different lesions and 5 non neurological controls from the manuscript (Jäkel & Agirre et. al., Altered human oligodendrocyte heterogeneity in multiple sclerosis)
Altered human oligodendrocyte heterogeneity in multiple sclerosis
Sarah Jäkel†, Eneritz Agirre†, Ana Mendanha Falcão, David van Bruggen, Ka Wai Lee, Irene Knuesel, Dheeraj Malhotra, Charles ffrench-Constant*, Anna Williams*, Gonçalo Castelo-Branco*
Nature, online 23 januari 2019
Oligodendrocyte lineage in a mouse model of multiple sclerosis
This dataset accompanies the manuscript below, published in Nature Medicine 2018. This dataset features single-cell transcriptomics data (SmartSeq2) of the oligodendrocyte lineage from the spinal cord of mice induced with experimental autoimmune encephalomyelitis (EAE), which mimics several aspects of MS.
Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis
Ana Mendanha Falcão* David van Bruggen*, Sueli Marques, Eneritz Agirre, Mandy Meijer, Sarah Jäkel, André Ortlieb Guerreiro-Cacais, Darya Vanichkina, Elisa M. Floriddia, Charles ffrench-Constant, Anna Williams, Gonçalo Castelo-Branco
Nature Medicine, 2018, in press
OPCs throughout mouse development
This dataset accompanies the manuscript below, deposited in BioRxiv and published in Developmental Cell in 2018. This dataset features bulk-RNA-seq data and single-cell transcriptomics data of time points E13.5, P7, and juvenile and adult mice, using STRT-Seq.
Sueli Marques, David van Bruggen, Darya Vanichkina, Elisa M. Floriddia, Hermany Munguba, Leif Väremo, Stefania Giacomello, Ana Mendanha Falcão, Mandy Meijer, Åsa K. Björklund, Jens Hjerling-Leffler, Ryan J. Taft, Gonçalo Castelo-Branco
Oligodendrocyte lineage in the juvenile and adult mouse CNS
This dataset accompanies the manuscript below, published in Science 2016. This dataset features single-cell transcriptomics data of juvenile and adult central nervous system, covering 10 different regions and approximately 5 thousands cells of the oligodendrocyte lineage, using STRT-Seq. The data and web resource was produced in collaboration with Sten Linnarsson’s lab.
Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system.
Marques S, Zeisel A, Codeluppi S, van Bruggen D, Mendanha Falcão A, Xiao L, et al
Science 2016 Jun;352(6291):1326-1329
We also recommend the following resources from Sten Linnarsson’s lab, which provide extraordinary insights of cells lineages in the CNS at a single cell level, including oligodendrocyte lineage:
And the pioneering resource from Ben Barres’s lab with bulk RNA-Seq.