Publications - Schulte lab

Selected publications

Wnt/β-catenin and NFκB signaling synergize to trigger growth-factor-free regeneration of adult primary human hepatocytes.
Oliva-Vilarnau N, Beusch CM, Sabatier P, Sakaraki E, Tjaden A, Graetz L, Büttner FA, Dorotea D, Nguyen M, Bergqvist F, Sundström Y, Müller S, Zubarev RA, Schulte G, Tredup C, Gramignoli R, Tietge UJF, Lauschke VM
Hepatology 2023 Oct;():

Pathway selectivity in Frizzleds is achieved by conserved micro-switches defining pathway-determining, active conformations.
Grätz L, Kowalski-Jahn M, Scharf MM, Kozielewicz P, Jahn M, Bous J, Lambert NA, Gloriam DE, Schulte G
Nat Commun 2023 Jul;14(1):4573

NanoBiT- and NanoBiT/BRET-based assays allow the analysis of binding kinetics of Wnt-3a to endogenous Frizzled 7 in a colorectal cancer model.
Grätz L, Sajkowska-Kozielewicz JJ, Wesslowski J, Kinsolving J, Bridge LJ, Petzold K, Davidson G, Schulte G, Kozielewicz P
Br J Pharmacol 2023 Apr;():

WNT stimulation induces dynamic conformational changes in the Frizzled-Dishevelled interaction.
Bowin CF, Kozielewicz P, Grätz L, Kowalski-Jahn M, Schihada H, Schulte G
Sci Signal 2023 Apr;16(779):eabo4974

Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain.
Kowalski-Jahn M, Schihada H, Turku A, Huber T, Sakmar TP, Schulte G.
Science Advances. 2021, 7, eabj7917. 10 Nov 2021

Residue 6.43 defines receptor function in class F GPCRs.
Turku A, Schihada H, Kozielewicz P, Bowin CF, Schulte G.
Nat Commun. 2021 Jun 24;12(1):3919

Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.
Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson G, Schulte G.
ACS Pharmacol Transl Sci. 2021 May 11;4(3):1235-1245. eCollection 2021 Jun 11

Cryo-EM structure of constitutively active human Frizzled 7 in complex with heterotrimeric Gs
Xu L, Chen B, Schihada H, Wright S C, Turku A, Wu Y, Han GW, Kowalski-Jahn M, Kozielewicz P, Bowin CF, Zhang X, Li C,Bouvier M, Schulte G and Xu F.
Cell Research (2021) 0:1–4

Deconvolution of WNT-induced Frizzled conformational dynamics with fluorescent biosensors.
Schihada H, Kowalski-Jahn M, Turku A, Schulte G
Biosens Bioelectron 2020 Dec;177():112948

eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled.
Wesslowski J, Kozielewicz P, Wang X, Cui H, Schihada H, Kranz D, et al
J Biol Chem 2020 06;295(26):8759-8774

Structural insight into small molecule action on Frizzleds.
Kozielewicz P, Turku A, Bowin CF, Petersen J, Valnohova J, Cañizal MCA, et al
Nat Commun 2020 Jan;11(1):414

A NanoBRET-Based Binding Assay for Smoothened Allows Real-time Analysis of Ligand Binding and Distinction of Two Binding Sites for BODIPY-cyclopamine.
Kozielewicz P, Bowin CF, Turku A, Schulte G
Mol. Pharmacol. 2020 Jan;97(1):23-34

WNT-3A-induced β-catenin signaling does not require signaling through heterotrimeric G proteins.
Bowin CF, Inoue A, Schulte G
J. Biol. Chem. 2019 Jun;():

A conserved molecular switch in Class F receptors regulates receptor activation and pathway selection.
Wright SC, Kozielewicz P, Kowalski-Jahn M, Petersen J, Bowin CF, Slodkowicz G, et al
Nat Commun 2019 02;10(1):667

FZD5 is a Gαq-coupled receptor that exhibits the functional hallmarks of prototypical GPCRs.
Wright SC, Cañizal MCA, Benkel T, Simon K, Le Gouill C, Matricon P, et al
Sci Signal 2018 Dec;11(559):

Dishevelled enables casein kinase 1-mediated phosphorylation of Frizzled 6 required for cell membrane localization.
Strakova K, Kowalski-Jahn M, Gybel T, Valnohova J, Dhople VM, Harnos J, et al
J. Biol. Chem. 2018 11;293(48):18477-18493

Functional dissection of the N-terminal extracellular domains of Frizzled 6 reveals their roles for receptor localization and Dishevelled recruitment.
Valnohova J, Kowalski-Jahn M, Sunahara RK, Schulte G
J. Biol. Chem. 2018 11;293(46):17875-17887

Agonist-induced dimer dissociation as a macromolecular step in G protein-coupled receptor signaling.
Petersen J, Wright SC, Rodríguez D, Matricon P, Lahav N, Vromen A, et al
Nat Commun 2017 08;8(1):226

The tyrosine Y2502.39 in Frizzled 4 defines a conserved motif important for structural integrity of the receptor and recruitment of Disheveled.
Strakova K, Matricon P, Yokota C, Arthofer E, Bernatik O, Rodriguez D, et al
Cell. Signal. 2017 10;38():85-96

FZD10-Gα13 signalling axis points to a role of FZD10 in CNS angiogenesis.
Hot B, Valnohova J, Arthofer E, Simon K, Shin J, Uhlén M, et al
Cell. Signal. 2017 04;32():93-103

WNT Stimulation Dissociates a Frizzled 4 Inactive-State Complex with Gα12/13.
Arthofer E, Hot B, Petersen J, Strakova K, Jäger S, Grundmann M, et al
Mol. Pharmacol. 2016 10;90(4):447-59

High levels of WNT-5A in human glioma correlate with increased presence of tumor-associated microglia/monocytes.
Dijksterhuis JP, Arthofer E, Marinescu VD, Nelander S, Uhlén M, Pontén F, et al
Exp. Cell Res. 2015 Dec;339(2):280-8

Systematic mapping of WNT-FZD protein interactions reveals functional selectivity by distinct WNT-FZD pairs.
Dijksterhuis JP, Baljinnyam B, Stanger K, Sercan HO, Ji Y, Andres O, et al
J. Biol. Chem. 2015 Mar;290(11):6789-98

Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity.
Kilander MB, Dahlström J, Schulte G
Cell. Signal. 2014 Sep;26(9):1943-9

Disheveled regulates precoupling of heterotrimeric G proteins to Frizzled 6.
Kilander MB, Petersen J, Andressen KW, Ganji RS, Levy FO, Schuster J, et al
FASEB J. 2014 May;28(5):2293-305

WNT-3A and WNT-5A counteract lipopolysaccharide-induced pro-inflammatory changes in mouse primary microglia.
Halleskog C, Schulte G
J. Neurochem. 2013 Jun;125(6):803-8

Pertussis toxin-sensitive heterotrimeric G(αi/o) proteins mediate WNT/β-catenin and WNT/ERK1/2 signaling in mouse primary microglia stimulated with purified WNT-3A.
Halleskog C, Schulte G
Cell. Signal. 2013 Apr;25(4):822-8

Heterotrimeric G protein-dependent WNT-5A signaling to ERK1/2 mediates distinct aspects of microglia proinflammatory transformation.
Halleskog C, Dijksterhuis JP, Kilander MB, Becerril-Ortega J, Villaescusa JC, Lindgren E, et al
J Neuroinflammation 2012 May;9():111

Recombinant WNTs differentially activate β-catenin-dependent and -independent signalling in mouse microglia-like cells.
Kilander MB, Halleskog C, Schulte G
Acta Physiol (Oxf) 2011 Nov;203(3):363-72

WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins.
Kilander MB, Dijksterhuis JP, Ganji RS, Bryja V, Schulte G
Cell. Signal. 2011 Mar;23(3):550-4

WNT signaling in activated microglia is proinflammatory.
Halleskog C, Mulder J, Dahlström J, Mackie K, Hortobágyi T, Tanila H, et al
Glia 2011 Jan;59(1):119-31

Beta-arrestin and casein kinase 1/2 define distinct branches of non-canonical WNT signalling pathways.
Bryja V, Schambony A, Cajánek L, Dominguez I, Arenas E, Schulte G
EMBO Rep. 2008 Dec;9(12):1244-50

Inhibition of endocytosis blocks Wnt signalling to beta-catenin by promoting dishevelled degradation.
Bryja V, Cajánek L, Grahn A, Schulte G
Acta Physiol (Oxf) 2007 May;190(1):55-61

Beta-arrestin is a necessary component of Wnt/beta-catenin signaling in vitro and in vivo.
Bryja V, Gradl D, Schambony A, Arenas E, Schulte G
Proc. Natl. Acad. Sci. U.S.A. 2007 Apr;104(16):6690-5

Review articles

Molecular Pharmacology of Class F Receptor Activation.
Kozielewicz P, Turku A, Schulte G
Mol Pharmacol 2020 02;97(2):62-71

Structural insight into Class F receptors - What have we learnt regarding agonist-induced activation?
Schulte G, Kozielewicz P
Basic Clin. Pharmacol. Toxicol. 2019 Mar

Frizzleds as GPCRs - More Conventional Than We Thought!
Schulte G, Wright SC
Trends Pharmacol. Sci. 2018 09;39(9):828-842

Frizzleds and WNT/β-catenin signaling--The black box of ligand-receptor selectivity, complex stoichiometry and activation kinetics.
Schulte G
Eur. J. Pharmacol. 2015 Sep;763(Pt B):191-5

WNT/Frizzled signalling: receptor-ligand selectivity with focus on FZD-G protein signalling and its physiological relevance: IUPHAR Review 3.
Dijksterhuis JP, Petersen J, Schulte G
Br. J. Pharmacol. 2014 Mar;171(5):1195-209

International Union of Basic and Clinical Pharmacology. LXXX. The class Frizzled receptors.
Schulte G
Pharmacol. Rev. 2010 Dec;62(4):632-67

beta-Arrestins - scaffolds and signalling elements essential for WNT/Frizzled signalling pathways?
Schulte G, Schambony A, Bryja V
Br. J. Pharmacol. 2010 Mar;159(5):1051-8

The Frizzled family of unconventional G-protein-coupled receptors.
Schulte G, Bryja V
Trends Pharmacol. Sci. 2007 Oct;28(10):518-25

Podcasts

The Dr. GPCR podcast #46: Gunnar Schulte interviewed by Dr Yamina Berchiche.

Listen here

Full publication list

Full list of publications - PubMed

Master's Theses and PhD projects

Master's theses and PhD projects from the Schulte lab