Skip to main content

Staffan Holmin's research group

Introduction

The Staffan Holmin research group is a part of the Department of Clinical Neuroscience at Karolinska Institutet and the Department of Neuroradiology at Karolinska University Hospital. The group is engaged in clinical and translational research on a wide variety of topics centered around modern neuroimaging, nuclear medicine, and image-guided vascular interventions.

Collaboration with Royal Institute of Technology

To enable technical development, we have extensive collaboration with researchers at the Department of Materials Science for prototyping and manufacture of novel endovascular equipment. We also have extensive collaboration with the research group Physics of Medical Imaging for the development of techniques in CT.

Projects

Clinical diagnostic and interventional neuroradiology

Clinical research is based at the Department of Neuroradiology at Karolinska University Hospital, and will continue at New Karolinska Hospital when the department relocates in 2018. Special areas of interest include pediatric neuroradiology, technical development in computed tomography (CT) and digital subtraction angiography, catheter-based chemotherapy in pediatric retinoblastoma, and thrombectomy in acute ischemic stroke.

Figure 1.

Clinical and experimental radiochemistry

The work of prof emeritus Sharon Stone-Elander is continued in the group, where radiotracer development and validation, and positron emission tomography (PET) in animal models of stroke, inflammation and cancer are areas of study. The group also has a significant clinical responsibility of radiotracer production for Karolinska University Hospital as well as other hospitals in the region.  Examples of produced radiotracers and areas of study include: 18F-FDG (glucose metabolism), 11C-methionine (amino acids), 11C-acetate (fatty acids), 18F-NAF (skeletal diseases), 18F-FLT (cell proliferation), 18F-FMISO (hypoxia), 68Ga-DOTATOC (NET/somatostatin receptor), 68Ga-PSMA (prostate cancer).

Our research projects

  1. Exploration of “interventional nuclear medicine”, i.e. combination of endovascular intervention and nuclear medicine technology.

  2. Developing of new diagnostic and therapeutic radiotracers 

  3. Evaluations of tracer utilization and metabolism in vitro and ex vivo

  4. Pharmacokinetic evaluations of new PET imaging radiotracers in vivo in animal models of disease (cancer, brain, inflammation, apoptosis, hypoxia, cardiology) without and with therapeutic interventions

Figur 2

Experimental interventional radiology and imaging

At the KERIC core facility, preclinical and translational research is conducted using state-of-the-art imaging such as a full scale angiocatheterization lab for animal research, micro-PET for small animals, and high field 9.4T magnetic resonance imaging (MRI). New devices and applications for endovascular interventional technique are explored.

Clinically relevant stroke model

We have developed an endovascular stroke (M2CAO) model in rat, using standard clinical equipment, wherein selective M2 segment occlusion is achieved and can be modulated with reperfusion to simulate thrombectomy. The model could be considered a successor to the filament MCAO model, but with greater control over infarct size and with preserved collateral circulation. The model is coupled to longitudinal MRI and PET imaging.

Trans-vessel wall technique

We have developed a specialized catheter system that enables direct transvascular access to tissue for deposition of cells or substances, without risk of bleeding. The system is compatible with standard endovascular equipment and enables effective minimally invasive cell transplantation.

Endovascular biopsy

We are developing new techniques and devices for endovascular biopsy of the vessel wall and endothelium, heart, and other organs. These devices aim to be less traumatic, and suitable for downstream analysis of tissues and single cells using modern molecular biology, such as RNA-sequencing (RNA-seq).

Selected Publications

Preserved Collateral Blood Flow in the Endovascular M2CAO Model Allows for Clinically Relevant Profiling of Injury Progression in Acute Ischemic Stroke.
Little P, Kvist O, Grankvist R, Jonsson S, Damberg P, Söderman M, et al
PLoS ONE 2017 ;12(1):e0169541

Patients with subarachnoid haemorrhage from vertebrobasilar dissection: treatment with stent-in-stent technique.
Bhogal P, Brouwer P, Söderqvist �, Ohlsson M, Andersson T, Holmin S, et al
Neuroradiology 2015 Jun;57(6):605-14

Superselective endovascular tissue access using trans-vessel wall technique: feasibility study for treatment applications in heart, pancreas and kidney in swine.
Grankvist R, Jensen-Urstad M, Clarke J, Lehtinen M, Little P, Lundberg J, et al
J. Intern. Med. 2019 Apr;285(4):398-406

Intra-arterial chemotherapy for retinoblastoma in Sweden - evaluation of treatment efficacy and complications.
Radros J, All-Eriksson C, Pal N, Holm S, Seregard S, Söderman M, et al
Acta Ophthalmol 2018 Dec;96(8):e1040-e1041

Treatment response assessment with (R)-[CPAQ PET in the MMTV-PyMT mouse model of breast cancer.
Tegnebratt T, Lu L, Eksborg S, Chireh A, Damberg P, Nikkhou-Aski S, et al
EJNMMI Res 2018 Apr;8(1):25

Group members

 

Håkan Almqvist Graduate Student
Tommy Andersson Associated
Fabian Arnberg Associated
Babic Drazenko Associated
Petra Brinkestål Associated
Patricius Brouwer Associated
Arvin Chireh PhD student
Peter Damberg Associated
Francesca De Luca Research assistant
Anna Falk Delgado Associated, Postdoc
Vamsi Krishna Gontu Graduate Student
Jonas Grafström Postdoc
Per Grane Associated
Rikard Grankvist PhD student
Åke Holmberg Associated
Staffan Holmin Research team leader, Professor/senior physician
Patrik Jarvoll Associated
Ida Jonsson Research assistant
Emma Jussing Graduate Student
Åsa Kuntze Söderqvist Associated
Anders Lilja Associated
Philip Little Graduate Student
Li Lu Associated
Johan Lundberg Associated
Victoria Lövljung Research assistant
Daniel Martin Munoz Graduate Student
Nuno Canto Moreira Associated
Arvid Nordenskjöld Associated
Erik Samén Associated, Postdoc
Anki Sandberg Nordqvist Associated
Mikael Sandell Graduate Student
Sharon Stone-Elander Professor emeritus
Fredrik Ståhl Graduate Student
Michael Söderman Associated
Tetyana Tegnebratt Associated
Thuy Tran Associated