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We study human B cells in health and disease - Qiang Pan-Hammarström

We focus on two main areas of research:
Primary immunodeficiency and B cell malignancy

Current projects:

Regulation of immunoglobulin class switch recombination in human B cells

This project is aimed at understanding the complex molecular mechanisms involved in DNA editing, repair and recombination during immunoglobulin class switch recombination (CSR) and somatic hypermutation (SHM) and their involvement in the pathophysiological processes leading to immunodeficiency, genome instability and cancer development in humans.

Video explaining non-homologous end joining in class switch recombination

Induced pluripotent stems cells a platform for personalized diagnosis and therapy in patients with primary immunodeficiency

microscopy image
An iPSC colony derived from IgAD patient's fibroblast cells. Photo: Qiang Pan-Hammarström's group

The project is aimed at reprogramming the fibroblasts derived from primary immunodeficiency patients into pluripotent stem (iPS) cells and re-differentiating these iPS cells into antibody-producing B cells. If successful, this study will provide a methodological platform for the study of human B cell development and for development of new therapies aiming at editing genes/cells in patients with a variety of other primary immunodeficiency diseases.

Discovery of therapeutic targets in B cell lymphoma by next generation sequencing

illustration in coulour showing notch signalling, cell migration, immune escape etc, in red and blue
Altered pathways in Hepatitis B virus - associated in diffuse large B cell lymphoma. Illustration: Qiang Pan Hammarström's group

The project is aimed at identifying potentially treatable molecular targets in mature B cell lymphomas (with focus on diffuse large B cell lymphoma, follicular lymphoma and mantle cell lymphoma) by applying high-throughput, next generation-sequencing technologies such as whole genome and whole exome sequencing, immune repertoire sequencing, RNA sequencing and single-cell RNA sequencing. The multiomic sequencing data will be further integrated with clinical data as well as functional assays to identify genes/pathways that can be used for disease classification and prediction and for the development of new targeted therapy.

Antibody therapy against COVID-19

The project is aimed at establishing a passive immunotherapy against coronavirus COVID19. To reach this overall goal, we will obtain blood samples from convalescent donors, i.e. people who have recovered from the infection, to isolate antibodies that can be used to prevent and to treat the disease. 

Group members

    Qiang Pan Hammarström

    Professor and Group leader

    Likun Du

    Laboratory Coordinator

    Jonathan Arias Fuenzalida

    Postdoctoral fellow

    Valentyn Oksenych

    Visiting scientist

    Weicheng Ren

    Senior Researcher

    Yanhong Su

    PhD Student

    Chunli Yang

    Visiting PhD student

    Xiaofei Ye

    PhD Student

    Hui Wan

    PhD Student

    Jingwei Yu

    Visiting Scientist

    Alumni

    Click here to view list of alumni

    Selected publications

    Genomic basis for RNA alterations in cancer.
    Calabrese C, Davidson NR, Demircioğlu D, Fonseca NA, He Y, et al
    Nature 2020 02;578(7793):129-136

    Genetic landscape of hepatitis B virus-associated diffuse large B-cell lymphoma.
    Ren W, Ye X, Su H, Li W, Liu D, Pirmoradian M, et al
    Blood 2018 06;131(24):2670-2681

    Reduced immunoglobulin gene diversity in patients with Cornelia de Lange syndrome.
    Björkman A, Du L, van der Burg M, Cormier-Daire V, Borck G, Pié J, et al
    J. Allergy Clin. Immunol. 2018 01;141(1):408-411.e8

    Combined immunodeficiency and Epstein-Barr virus-induced B cell malignancy in humans with inherited CD70 deficiency.
    Abolhassani H, Edwards ES, Ikinciogullari A, Jing H, Borte S, Buggert M, et al
    J. Exp. Med. 2017 01;214(1):91-106

    Common variants at PVT1, ATG13-AMBRA1, AHI1 and CLEC16A are associated with selective IgA deficiency.
    Bronson PG, Chang D, Bhangale T, Seldin MF, Ortmann W, Ferreira RC, et al
    Nat. Genet. 2016 11;48(11):1425-1429

    Genetic basis of PD-L1 overexpression in diffuse large B-cell lymphomas.
    Georgiou K, Chen L, Berglund M, Ren W, de Miranda NF, Lisboa S, et al
    Blood 2016 06;127(24):3026-34

    Frequent alterations in cytoskeleton remodelling genes in primary and metastatic lung adenocarcinomas.
    Wu K, Zhang X, Li F, Xiao D, Hou Y, Zhu S, et al
    Nat Commun 2015 Dec;6():10131

    Aberrant recombination and repair during immunoglobulin class switching in BRCA1-deficient human B cells.
    Björkman A, Qvist P, Du L, Bartish M, Zaravinos A, Georgiou K, et al
    Proc. Natl. Acad. Sci. U.S.A. 2015 Feb;112(7):2157-62

    B cell super-enhancers and regulatory clusters recruit AID tumorigenic activity.
    Qian J, Wang Q, Dose M, Pruett N, Kieffer-Kwon KR, Resch W, et al
    Cell 2014 Dec;159(7):1524-37

    Exome sequencing reveals novel mutation targets in diffuse large B-cell lymphomas derived from Chinese patients.
    de Miranda NF, Georgiou K, Chen L, Wu C, Gao Z, Zaravinos A, et al
    Blood 2014 Oct;124(16):2544-53

    A regulatory role for the cohesin loader NIPBL in nonhomologous end joining during immunoglobulin class switch recombination.
    Enervald E, Du L, Visnes T, Björkman A, Lindgren E, Wincent J, et al
    J. Exp. Med. 2013 Nov;210(12):2503-13

    New facets of antibody deficiencies.
    Liadaki K, Sun J, Hammarström L, Pan-Hammarström Q
    Curr. Opin. Immunol. 2013 Oct;25(5):629-38

    DNA repair genes are selectively mutated in diffuse large B cell lymphomas.
    de Miranda NF, Peng R, Georgiou K, Wu C, Falk Sörqvist E, Berglund M, et al
    J. Exp. Med. 2013 Aug;210(9):1729-42

    Nurture your scientific curiosity early in your research career.
    Jagodic M, Stridh P, Gad AK, Paine A, Udekwu KI, Sjöholm LK, et al
    Nat. Genet. 2013 Feb;45(2):116-8

    Cernunnos influences human immunoglobulin class switch recombination and may be associated with B cell lymphomagenesis.
    Du L, Peng R, Björkman A, Filipe de Miranda N, Rosner C, Kotnis A, et al
    J. Exp. Med. 2012 Feb;209(2):291-305

    Looking for a postdoc position?

    Postdoc scholarship position on RNA editing and COVID-19 available.

    For details contact: Prof. Pan-Hammarström

    Research techniques

    • DNA sequencing, including whole exome and whole genome sequencing

    • Gene expression analysis including realtime PCR and RNAseq/transcriptome analysis

    • B and T cell functional assays

    • Generating iPSCs

    • Gene editing using the CRISPR/Cas9 system