In our lab, we study the structural and functional properties of neocortical and striatal microcircuits, as well the interactions between these two brain areas (cortico-striatal pathway).
We use electrophysiological, anatomical, and imaging techniques in slices and in vivo, as well as computational methods in order to reveal the intricate organization of neurons and their synaptic connections. Our aim is to unravel the functional microcircuitry underlying sensorimotor processing in health and disease.
The Neocortex and Basal Ganglia are two brain regions involved in sensorimotor processing, and are tightly linked to each other via the cortico-striatal pathway. In order to understand the function of these brain regions, how they integrate sensory input and generate the appropriate motor output, it is essential to have a deep knowledge of their respective microcircuits.
Key topics in our research are:
- The properties and functional role of interneurons and their interaction with the projection neurons (medium spiny neurons in the striatum, pyramidal neurons in the neocortex).
- Synaptic dynamics and their affect on microcircuit operation.
- Feed-back and feed-forward inhibitory synaptic pathways.
- Short- and long-term synaptic plasticity in corticostriatal synapses.
- Sensory integration in Basal Ganglia networks.
Recent and ongoing research projects
Multisensory integration in the Striatum
Inhibitory pathways in the Striatum
Example of a simultaneous patch clamp recording from 4 striatal neurons. Stimulation of one striatal interneuron (Fast Spiking cell) evokes inhibitory responses in neighboring medium spiny neurons (MSNs) of both direct and indirect projections types (right). These responses are monosynaptic GABAergic IPSPs acting as a feedforward inhibitory pathway.
Inhibitory pathways in the Neocortex
Stimulation of a layer 5 pyramidal cell (PC) evokes disynaptic inhibitory responses in neighboring PCs. These responses are mediated by GABAergic Martinotti cells.
- Ramon Reig
- Jie Mei
- Mikko Koskinen
- Ian Olson
- Tiago Ferreira
- Susanne Szydlowski
- Ming Zhou
- Stylianos Papaioannou
- Henrike Planert
- Jesper Ericsson
- Andreas Klaus
- Julia Oyrer
- Emelie Braun
- Jeroen Brus
- Knut and Alice Wallenberg Foundation (Wallenberg Academy Fellowship)
- StratNeuro - The Strategic Research Program in Neuroscience at Karolinska Institutet
- Swedish Medical Research Council (VR-M)
- Karolinska Institutet
- European Research Council (ERC)
- Human Frontier Science Program (HFSP)
- Stockholm Brain Institute (SBI)
- Åke Wiberg foundation
- Magnus Bergvall foundation
- Network of European Neuroscience Institutes (ENI-net)
- Jeanssons Stiftelser
- EU FP7 – “Select and Act”
Differential Synaptic Input to External Globus Pallidus Neuronal Subpopulations In Vivo.
Ketzef M, Silberberg G
Neuron 2020 Nov;(): doi:10.1016/j.neuron.2020.11.006.
Polysynaptic inhibition between striatal cholinergic interneurons shapes their network activity patterns in a dopamine-dependent manner.
Dorst MC, Tokarska A, Zhou M, Lee K, Stagkourakis S, Broberger C, et al
Nat Commun 2020 10;11(1):5113
Synaptic Connectivity between the Cortex and Claustrum Is Organized into Functional Modules.
Chia Z, Augustine GJ, Silberberg G
Curr. Biol. 2020 Jun;(): 10.1016/j.cub.2020.05.031
The Functional Organization of Cortical and Thalamic Inputs onto Five Types of Striatal Neurons Is Determined by Source and Target Cell Identities.
Johansson Y, Silberberg G
Cell Rep 2020 Jan;30(4):1178-1194.e3
A New Micro-holder Device for Local Drug Delivery during In Vivo Whole-cell Recordings.
Sáez M, Ketzef M, Alegre-Cortés J, Reig R, Silberberg G
Neuroscience 2018 06;381():115-123
Dopamine Depletion Impairs Bilateral Sensory Processing in the Striatum in a Pathway-Dependent Manner.
Ketzef M, Spigolon G, Johansson Y, Bonito-Oliva A, Fisone G, Silberberg G
Neuron 2017 May;94(4):855-865.e5
Functional properties, topological organization and sexual dimorphism of claustrum neurons projecting to anterior cingulate cortex.
Chia Z, Silberberg G, Augustine, GJ
Claustrum 2, 1357412 (2017)
Optogenetic dissection of the striatal microcircuitry.
Silberberg G, Planert H
Advanced Patch-Clamp Analysis for Neuroscientists vol. 113 151–170 (2016)
Distinct Corticostriatal and Intracortical Pathways Mediate Bilateral Sensory Responses in the Striatum.
Reig R, Silberberg G
Cereb. Cortex 2016 12;26(12):4405-4415
Local and afferent synaptic pathways in the striatal microcircuitry.
Silberberg G, Bolam JP
Curr. Opin. Neurobiol. 2015 Aug;33():182-7
Multisensory integration in the mouse striatum.
Reig R, Silberberg G
Neuron 2014 Sep;83(5):1200-12
Target Selectivity of Feedforward Inhibition by Striatal Fast-Spiking Interneurons.
Szydlowski SN, Pollak Dorocic I, Planert H, Carlén M, Meletis K, Silberberg G
J. Neurosci. 2013 Jan;33(4):1678-83
Membrane properties of striatal direct and indirect pathway neurons in mouse and rat slices and their modulation by dopamine.
Planert H, Berger TK, Silberberg G
PLoS ONE 2013 ;8(3):e57054
Dynamics of synaptic transmission between fast-spiking interneurons and striatal projection neurons of the direct and indirect pathways.
Planert H, Szydlowski SN, Hjorth JJ, Grillner S, Silberberg G
J. Neurosci. 2010 Mar;30(9):3499-507
Full list of publications
Postdoc positions available
Our lab uses electrophysiological, morphological, optogenetic, and computational methods to study neural microcircuits in the neocortex and basal-ganglia. In particular we are interested in the dynamic properties of neuronal microcircuits underlying sensory and motor processing.
We study the dynamic interactions between different types of excitatory and inhibitory neurons in order to unravel the way neural networks are structured and dynamically orchestrated.
Postdoc candidates should have a strong neuroscience background with documented experience in patch-clamp recording and/or in vivo electrophysiology.
Knowledge of neuroanatomy, imaging, and computer programming are highly advantageous.
The project will involve in vivo patch-clamp recordings and calcium imaging in cortex and striatum.
Funding is guaranteed for the first 2 years and may be extended according to progress and available funding.
Karolinska Institutet Campus Solna
171 65 Stockholm