Silberberg Lab

In our lab, we study the structural and func­tional properties of neocortical and striatal microcircuits, as well the interactions between these two brain areas (cortico-striatal pathway).

Collage of image illustrating research at Silberberg lab

Research focus

We use electrophysiological, anatomical, and imaging techniques in slices and in vivo, as well as computational methods in order to reveal the intricate organization of neurons and their synaptic connections. Our aim is to unravel the functional microcircuitry underlying sensori­motor processing in health and disease.

The Neocortex and Basal Ganglia are two brain regions involved in sensorimotor processing, and are tightly linked to each other via the cortico-striatal pathway. In order to understand the function of these brain regions, how they integrate sensory input and generate the appropriate motor output, it is essential to have a deep knowledge of their respective micro­circuits.

Key topics in our research are:

  • The properties and functional role of inter­neurons and their interaction with the projection neurons (medium spiny neurons in the striatum, pyramidal neurons in the neocortex).
  • Synaptic dynamics and their affect on microcircuit operation.
  • Feed-back and feed-forward inhibitory synaptic pathways.
  • Short- and long-term synaptic plasticity in corticostriatal synapses.
  • Sensory integration in Basal Ganglia networks.

Recent and ongoing research projects

Multisensory integration in the Striatum.
Multisensory integration in the Striatum. Photo: Gilad Silberberg.

Multisensory integration in the Striatum

    Two figures illustrating Inhibitory pathways in the Stratium
    Inhibitory pathways in the Stratium (fig. 1 & 2).

    Inhibitory pathways in the Striatum

      Inhibitory pathways in the Striatum (fig. 3)
      Inhibitory pathways in the Striatum (fig. 3) Photo: Gilad Silberberg

      Example of a simultaneous patch clamp recording from 4 striatal neurons. Stimulation of one striatal interneuron (Fast Spiking cell) evokes inhibitory responses in neighboring medium spiny neurons (MSNs) of both direct and indirect projections types (right). These responses are monosynaptic GABAergic IPSPs acting as a feedforward inhibitory pathway.

      Inhibitory pathways in the Neocortex

        Inhibitory pathways in the Neocortex (fig. 1)
        Inhibitory pathways in the Neocortex (fig. 1) Photo: Gilad Silberberg
        Cover of Neuron (Mar 01, 2007, Volume 53, Issue 5, p. 619-770)
        Cover of Neuron (Mar 01, 2007, Volume 53, Issue 5, p. 619-770) Photo: Gilad Silberberg

        Stimulation of a layer 5 pyramidal cell (PC) evokes disynaptic inhibitory responses in neighboring PCs. These responses are mediated by GABAergic Martinotti cells.

          Group members

          Alumni

          • Ramon Reig
          • Jie Mei
          • Mikko Koskinen
          • Ian Olson
          • Tiago Ferreira
          • Susanne Szydlowski
          • Ming Zhou
          • Stylianos Papaioannou
          • Henrike Planert
          • Jesper Ericsson
          • Andreas Klaus
          • Julia Oyrer
          • Emelie Braun
          • Jeroen Brus

          Funding

          Current

          • Knut and Alice Wallenberg Foundation (Wallenberg Academy Fellowship)
          • StratNeuro - The Strategic Research Program in Neuroscience at Karolinska Institutet
          • Swedish Medical Research Council (VR-M)
          • Hjärnfonden
          • Karolinska Institutet

          Previous

          • European Research Council (ERC)
          • Human Frontier Science Program (HFSP)
          • Stockholm Brain Institute (SBI)
          • Åke Wiberg foundation
          • Magnus Bergvall foundation
          • Network of European Neuroscience Institutes (ENI-net)
          • Jeanssons Stiftelser
          • EU FP7 – “Select and Act”

          Selected publications

          Differential Synaptic Input to External Globus Pallidus Neuronal Subpopulations In Vivo.
          Ketzef M, Silberberg G
          Neuron 2020 Nov;(): doi:10.1016/j.neuron.2020.11.006.

          Polysynaptic inhibition between striatal cholinergic interneurons shapes their network activity patterns in a dopamine-dependent manner.
          Dorst MC, Tokarska A, Zhou M, Lee K, Stagkourakis S, Broberger C, et al
          Nat Commun 2020 10;11(1):5113

          Synaptic Connectivity between the Cortex and Claustrum Is Organized into Functional Modules.
          Chia Z, Augustine GJ, Silberberg G
          Curr. Biol. 2020 Jun;(): 10.1016/j.cub.2020.05.031

          The Functional Organization of Cortical and Thalamic Inputs onto Five Types of Striatal Neurons Is Determined by Source and Target Cell Identities.
          Johansson Y, Silberberg G
          Cell Rep 2020 Jan;30(4):1178-1194.e3

          A New Micro-holder Device for Local Drug Delivery during In Vivo Whole-cell Recordings.
          Sáez M, Ketzef M, Alegre-Cortés J, Reig R, Silberberg G
          Neuroscience 2018 06;381():115-123

          Dopamine Depletion Impairs Bilateral Sensory Processing in the Striatum in a Pathway-Dependent Manner.
          Ketzef M, Spigolon G, Johansson Y, Bonito-Oliva A, Fisone G, Silberberg G
          Neuron 2017 May;94(4):855-865.e5

          Functional properties, topological organization and sexual dimorphism of claustrum neurons projecting to anterior cingulate cortex.
          Chia Z, Silberberg G, Augustine, GJ
          Claustrum 2, 1357412 (2017)

          Optogenetic dissection of the striatal microcircuitry.
          Silberberg G, Planert H 
          Advanced Patch-Clamp Analysis for Neuroscientists vol. 113 151–170 (2016)

          Distinct Corticostriatal and Intracortical Pathways Mediate Bilateral Sensory Responses in the Striatum.
          Reig R, Silberberg G
          Cereb. Cortex 2016 12;26(12):4405-4415

          Local and afferent synaptic pathways in the striatal microcircuitry.
          Silberberg G, Bolam JP
          Curr. Opin. Neurobiol. 2015 Aug;33():182-7

          Multisensory integration in the mouse striatum.
          Reig R, Silberberg G
          Neuron 2014 Sep;83(5):1200-12

          Target Selectivity of Feedforward Inhibition by Striatal Fast-Spiking Interneurons.
          Szydlowski SN, Pollak Dorocic I, Planert H, Carlén M, Meletis K, Silberberg G
          J. Neurosci. 2013 Jan;33(4):1678-83

          Membrane properties of striatal direct and indirect pathway neurons in mouse and rat slices and their modulation by dopamine.
          Planert H, Berger TK, Silberberg G
          PLoS ONE 2013 ;8(3):e57054

          Dynamics of synaptic transmission between fast-spiking interneurons and striatal projection neurons of the direct and indirect pathways.
          Planert H, Szydlowski SN, Hjorth JJ, Grillner S, Silberberg G
          J. Neurosci. 2010 Mar;30(9):3499-507

          Full list of publications

          Full list of publications from Google Scholar

          Available positions

          Postdoc positions available

          Research

          Our lab uses electrophysiological, morphological, optogenetic, and computational methods to study neural microcircuits in the neocortex and basal-ganglia. In particular we are interested in the dynamic properties of neuronal microcircuits underlying sensory and motor processing.

          We study the dynamic interactions between different types of excitatory and inhibitory neurons in order to unravel the way neural networks are structured and dynamically orchestrated.

          Postdoc candidates should have a strong neuroscience background with documented experience in patch-clamp recording and/or in vivo electrophysiology.

          Knowledge of neuroanatomy, imaging, and computer programming are highly advantageous.

          The project will involve in vivo patch-clamp recordings and calcium imaging in cortex and striatum.

          Funding is guaranteed for the first 2 years and may be extended according to progress and available funding.

          Contact

          Visiting address

          Karolinska Institutet Campus Solna
          Biomedicum
          Quarter B4
          Solnavägen 9
          171 65 Stockholm

          Where to find us

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