Research Group Lanner lab

Developmental and Regenerative Stem cell Medicine

Aim

The Lanner lab aims to

  1. Uncover the fundamental principles underlying how the first cell types, totipotency and pluripotency is established in the early human embryo (1-4). We are increasingly using stem cell models to build embryo models (5).
  2. Develop embryonic stem cell-based strategies. We have established a first clinically compliant good manufacturing practice (GMP) grade human embryonic stem cell line, KARO1. KARO1 is now available for collaborators in the stage of clinical translation. We are currently looking into developing genetically engineered versions to combat transplantation rejection (6,7).
  3. Treatment of Diabetes. We are actively working to establish methodology both to efficiently produce glucose responsive beta cells and evaluate the optimal way to deliver these to the patient.
  4. Treatment of age-related macular degeneration (AMD). We have developed highly efficient differentiation protocol to generate retinal pigmented epithelial (RPE) cells for treatment of macular degeneration (8, 9). Based on this we have enter a collaborative agreement with St. Erik Eye Hospital and Novo Nordisk A/S to manufacture hESC-RPE cells (CellThRPE1) to conduct a first-in-human clinical trial aiming to treat AMD. We continue to advance related methodologies and increase our fundamental knowledge of how the different cell types of the retina are established during embryogenesis and in vitro (10).

Key publications

1. Single-cell RNA-seq reveals lineage and X-chromosome dynamics in human preimplantation embryos S Petropoulos, D Edsgärd, B Reinius, Q Deng, SP Panula, S Codeluppi, A Plaza Reyes, S Linnarsson, R Sandberg, F Lanner Cell, 2016

2. Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States. Collier AJ, Panula SP, Schell JP, Chovanec P, Plaza Reyes A, Petropoulos S, Corcoran AE, Walker R, Douagi I, Lanner F, Rugg-Gunn PJ. Cell Stem Cell. 2017

3. Amnion signals are essential for mesoderm formation in primates Yang R, Goedel A, Kang Y, Si C, Chu C, Zheng Y, Chen Z, Gruber PJ, Xiao Y, Zhou C, Witman N, Leung CY, Chen Y, Fu J, Ji W, Lanner F, Niu Y, Chien K. BioRxiv 2020

4. The E-cadherin/AmotL2 complex organizes actin filaments required for epithelial hexagonal packing and blastocyst hatching S Hildebrand, S Hultin, A Subramani, S Petropoulos, Y Zhang, X Cao, J Mpindi, O Kalloniemi, S Johansson, A Majumdar, F Lanner & L Holmgren. Scientific Reports 2017

5. Evaluating totipotency using criteria of increasing stringency Posfai E, Schell JP, Janiszewski A, Rovic I, Murray A, Bradshaw B, Yamakawa T, Pardon T, El Bakkali M, Talon I, De Geest N, Kumar P, To SK, Petropoulos S, Jurisicova A, Pasque V, Lanner F and Janet Rossant. Nature Cell Biology, 2021

6. Karolinska Institutet Human Embryonic Stem Cell Bank Main H, Hedenskog M, Acharya G, Hovatta O, Lanner F. Stem Cell Research. 2020

7. Generation of Retinal Pigment Epithelial Cells Derived from Human Embryonic Stem Cells Lacking Human Leukocyte Antigen Class I and II. Petrus-Reurer S, Winblad N, Kumar P, Gorchs L, Chrobok M, Wagner AK, Bartuma H, Lardner E, Aronsson M, Plaza Reyes Á, André H, Alici E, Kaipe H, Kvanta A, Lanner F. Stem Cell Reports. 2020

8. Xeno-Free and Defined Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells Functionally Integrate in a Large-Eyed Preclinical Model. A Plaza Reyes, S Petrus-Reurer, L Antonsson, S Stenfelt, H Bartuma, S Panula, T Mader, I Douagi, H André, O Hovatta, F Lanner, A Kvanta. Stem Cell Reports, 2016

9. Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells. Plaza Reyes A, Petrus-Reurer S, Padrell Sánchez S, Kumar P, Douagi I, Bartuma H, Aronsson M, Westman S, Lardner E, André H, Falk A, Nandrot EF, Kvanta A, Lanner F. Nat Commun. 2020

10. Molecular profiling of retinal pigment epithelial cell differentiation for therapeutic use Petrus-Reurer S, Lederer AR, Baqué-Vidal L, Douagi I, Pannagel B, Aronsson M, Bartuma H, Wagner M, André H, Sundström E, Bhaduri A, Kriegstein A, Kvanta A, La Manno G, Lanner F. BioRxiv 2021