Robert Månsson group

Our research mainly focuses around the developmental progression from stem and progenitor cells into mature B-lymphoid cells and the transcriptional networks that drive this process.

Our research

Our research mainly focuses around the developmental progression from stem and progenitor cells into mature B-lymphoid cells and the transcriptional networks that drive this process. We study this through a combination of in vivo models and cell- and molecular biology techniques. The overall aim is to understand the differentiation process and through this increase our understanding of diseases that originate from developmental defects such as cancer and various immunodeficiencies.

The hematopoietic system is maintained through the continuous generation of new mature effector cells from self-renewing multipotent progenitors. A major challenge for the field is to understand mechanisms behind this differentiation process

Keywords:

hematopoietic progenitor, B-cell development, transcriptional network, transcription factor, differentiation, interacting regulatory elements

Protocols

High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers.

Gustafsson C, De Paepe A, Schmidl C, Månsson R.
BMC Genomics. 2019 Jan 18;20(1):59. doi: 10.1186/s12864-018-5299-0.

Link to the full publication

Public Data Sets

For easier navigation in ENA browser, click Show selected columns and mark Sample Alias.

The Concerted Action of E2-2 and HEB Is Critical for Early Lymphoid Specification.

Bouderlique T, Peña-Pérez L, Kharazi S, Hils M, Li X, Krstic A, De Paepe A, Schachtrup C, Gustafsson C, Holmberg D, Schachtrup K, Månsson R.
Front Immunol. 2019 Mar 18;10:455. doi: 10.3389/fimmu.2019.00455.
https://www.ebi.ac.uk/ena/browser/view/PRJEB20316
https://www.ebi.ac.uk/ena/browser/view/PRJEB29568

High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers.

Gustafsson C, De Paepe A, Schmidl C, Månsson R.
BMC Genomics. 2019 Jan 18;20(1):59. doi: 10.1186/s12864-018-5299-0.
https://www.ebi.ac.uk/ena/browser/view/PRJEB23059

Active enhancer and chromatin accessibility landscapes chart the regulatory network of primary multiple myeloma.

Jin Y, Chen K, De Paepe A, Hellqvist E, Krstic AD, Metang L, Gustafsson C, Davis RE, Levy YM, Surapaneni R, Wallblom A, Nahi H, Mansson R, Lin YC.
Blood. 2018 May 10;131(19):2138-2150. doi: 10.1182/blood-2017-09-808063.
https://www.ebi.ac.uk/ena/browser/view/PRJEB25605

Mice deficient of Myc super-enhancer region reveal differential control mechanism between normal and pathological growth.

Dave K, Sur I, Yan J, Zhang J, Kaasinen E, Zhong F, Blaas L, Li X, Kharazi S, Gustafsson C, De Paepe A, Månsson R, Taipale J.
Elife. 2017 Jun 6;6. pii: e23382. doi: 10.7554/eLife.23382.
https://www.ebi.ac.uk/ena/browser/view/PRJEB20316
https://www.ebi.ac.uk/ena/browser/view/PRJEB11397

HOMER

HOMER-Software for motif discovery and next generation sequencing analysis

Publications

Selected publications

Staff and contact

Group leader

All members of the group

Open positions

We always want to get in touch with talented potential co-workers. If you are interested in doing research within our group, as a degree project or as a researcher, please contact the group leader Robert Månsson: robert.mansson@ki.se

Projects

The hematopoietic system is maintained through the continuous generation of new mature effector cells from self-renewing multipotent progenitors. Development of mature cells occur gradually through series of hierarchically related developmental stages.

This gradual process coupled with the relative ease by which different developmental stages can be purified (based on stage and lineage specific surface markers) make hematopoiesis an ideal system for studying developmental progression. In particular the development of B-cells has been very well characterized leading to the identification of several successive stages of B-cell development. In addition a hierarchically organized network of transcription factors has been identified. This network includes several transcription factors (EBF1, E2A, PAX5, FOXO1 and others), all demonstrated to be critical for proper development and commitment to the B-cell lineage. Together this makes the development of B-cell possibly the most well understood developmental pathways to date.

However, even though the developmental pathway is well defined, our understanding of the molecular mechanisms that drive differentiation and lineage specification is still limited. Thus, a major challenge for the field is to understand how elaborate transcriptional programs drive these processes through temporal and cell-type specific gene expression.

Studies will explore the developmental progression from stem and progenitor cells into mature B-lymphoid cells and the transcriptional networks that drive this process.

More specifically, studies will be aimed at:

  1. Increasing our ability to resolve discrete stages of developmental progression
  2. Using high resolution cell separation, coupled to genome wide approaches, to study changes in transcription factor usage with resulting effects on epigenetic marks and transcription in association with developmental progression
  3. Identification and study of distal regulatory elements and how these interact with and modulate their transcriptional targets