Our research
Our research mainly focuses around the developmental progression from stem and progenitor cells into mature B-lymphoid cells and the transcriptional networks that drive this process. We study this through a combination of in vivo models and cell- and molecular biology techniques. The overall aim is to understand the differentiation process and through this increase our understanding of diseases that originate from developmental defects such as cancer and various immunodeficiencies.
The hematopoietic system is maintained through the continuous generation of new mature effector cells from self-renewing multipotent progenitors. A major challenge for the field is to understand mechanisms behind this differentiation process
Keywords:
hematopoietic progenitor, B-cell development, transcriptional network, transcription factor, differentiation, interacting regulatory elements
Protocols
High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers.
Gustafsson C, De Paepe A, Schmidl C, Månsson R.
BMC Genomics. 2019 Jan 18;20(1):59. doi: 10.1186/s12864-018-5299-0.
Public Data Sets
For easier navigation in ENA browser, click Show selected columns and mark Sample Alias.
The Concerted Action of E2-2 and HEB Is Critical for Early Lymphoid Specification.
Bouderlique T, Peña-Pérez L, Kharazi S, Hils M, Li X, Krstic A, De Paepe A, Schachtrup C, Gustafsson C, Holmberg D, Schachtrup K, Månsson R.
Front Immunol. 2019 Mar 18;10:455. doi: 10.3389/fimmu.2019.00455.
https://www.ebi.ac.uk/ena/browser/view/PRJEB20316
https://www.ebi.ac.uk/ena/browser/view/PRJEB29568
High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers.
Gustafsson C, De Paepe A, Schmidl C, Månsson R.
BMC Genomics. 2019 Jan 18;20(1):59. doi: 10.1186/s12864-018-5299-0.
https://www.ebi.ac.uk/ena/browser/view/PRJEB23059
Active enhancer and chromatin accessibility landscapes chart the regulatory network of primary multiple myeloma.
Jin Y, Chen K, De Paepe A, Hellqvist E, Krstic AD, Metang L, Gustafsson C, Davis RE, Levy YM, Surapaneni R, Wallblom A, Nahi H, Mansson R, Lin YC.
Blood. 2018 May 10;131(19):2138-2150. doi: 10.1182/blood-2017-09-808063.
https://www.ebi.ac.uk/ena/browser/view/PRJEB25605
Mice deficient of Myc super-enhancer region reveal differential control mechanism between normal and pathological growth.
Dave K, Sur I, Yan J, Zhang J, Kaasinen E, Zhong F, Blaas L, Li X, Kharazi S, Gustafsson C, De Paepe A, Månsson R, Taipale J.
Elife. 2017 Jun 6;6. pii: e23382. doi: 10.7554/eLife.23382.
https://www.ebi.ac.uk/ena/browser/view/PRJEB20316
https://www.ebi.ac.uk/ena/browser/view/PRJEB11397