Neural stem cells – Anna Falk group

Research focus

A human patient iPS cell colony stained for pluripotency markers Oct4 in green and Tra1-81 in red. Photo M. Shahsavani.

Research on Neurodevelopment

Using reprogramming to model diseases of the human brain

Reprogramming somatic cells to induced pluripotent stem (iPS) cells and directed differentiation opens up for studies on neural cells from patients. 

The Falk group is focusing on fundamental questions in early human neurogenesis:

• How do neural stem cells make their fate choices between self-renewal and differentiation? Between neurons and glia? How are these decisions hampered in neurodevelopmental and neuropsychiatric disorders? Are in vitro human neurogenesis, migration and maturation in 2D and/or 3D systems mimicking development in health and disease?
• The regenerative potential of iPS derived cell types and the routes forward to clinic 

We build cellular models of the healthy and diseased human brain using reprogrammed patient cells.

We derive patient specific iPS cells that are further differentiated into patient specific neuroepithelial stem (NES) cells.

Both iPS and NES cell lines can be robustly expanded in culture and by applying efficient neuronal differentiation protocols we derive close to pure cultures of unlimited numbers of neurons.

We use the cellular models to study proliferation, self-renewal, and differentiation potential as well as the function and subtype of derived neurons to uncover disease mechanisms. 

The Falk lab is also driving translational projects investigating the regenerative potential of iPS cell derived neural cells in preclinical models with the aim to create ATMPs to take iPS derived cell to clinic. We have derived one of few clinical compliant GMP iPS cell lines that we have banked in a master bank and we are now investigating the route forward to clinic.

Publications

Selected publications

• Article: CELL STEM CELL. 2019;25(6):855-870.e11

  Humanized Stem Cell Models of Pediatric Medulloblastoma Reveal an Oct4/mTOR Axis that Promotes Malignancy

  Cancer M; Hutter S; Holmberg KO; Rosen G; Sundstrom A; Tailor J; Bergstrom T; Garancher A; Essand M; Wechsler-Reya RJ; Falk A; Weishaupt H; Swartling FJ
• Article: EXPERIMENTAL CELL RESEARCH. 2019;383(1):111469

  Single cell analysis of autism patient with bi-allelic NRXN1-alpha deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality

  Lam M; Moslem M; Bryois J; Pronk RJ; Uhlin E; Ellstrom ID; Laan L; Olive J; Morse R; Ronnholm H; Louhivuori L; Korol SV; Dahl N; Uhlen P; Anderlid B-M; Kele M; Sullivan PF; Falk A
• Article: FRONTIERS IN GENETICS. 2019;10:896

  Ataxia in Patients With Bi-Allelic NFASC Mutations and Absence of Full-Length NF186

  Kvarnung M; Shahsavani M; Taylan F; Moslem M; Breeuwsma N; Laan L; Schuster J; Jin Z; Nilsson D; Lieden A; Anderlid B-M; Nordenskjold M; Lundberg ES; Birnir B; Dahl N; Nordgren A; Lindstrand A; Falk A
• Article: MOLECULAR PSYCHIATRY. 2018;23(7):1674-1684

  An in vitro model of lissencephaly: expanding the role of DCX during neurogenesis

  Shahsavani M; Pronk RJ; Falk R; Lam M; Moslem M; Linker SB; Salma J; Day K; Schuster J; Anderlid B-M; Dahl N; Gage FH; Falk A
• Article: STEM CELL REPORTS. 2018;10(3):1030-1045

  Human iPS-Derived Astroglia from a Stable Neural Precursor State Show Improved Functionality Compared with Conventional Astrocytic Models

  Lundin A; Delsing L; Clausen M; Ricchiuto P; Sanchez J; Sabirsh A; Ding M; Synnergren J; Zetterberg H; Brolen G; Hicks R; Herland A; Falk A
• Article: STEM CELL RESEARCH. 2017;18:22-25

  Derivation of human iPS cell lines from monozygotic twins in defined and xeno free conditions

  Uhlin E; Ronnholm H; Day K; Kele M; Tammimies K; Bolte S; Falk A
• Article: BIOMATERIALS. 2014;35(30):8496-8502

  Spider silk for xeno-free long-term self-renewal and differentiation of human pluripotent stem cells

  Wu S; Johansson J; Damdimopoulou P; Shahsavani M; Falk A; Hovatta O; Rising A
• Article: BIOMATERIALS. 2014;35(5):1420-1428

  A 3D Alzheimer's disease culture model and the induction of P21-activated kinase mediated sensing in iPSC derived neurons

  Zhang D; Pekkanen-Mattila M; Shahsavani MO; Falk A; Teixeira AI; Herland A
• Article: JOURNAL OF NEUROSCIENCE. 2013;33(30):12407-12422

  Stem Cells Expanded from the Human Embryonic Hindbrain Stably Retain Regional Specification and High Neurogenic Potency

  Tailor J; Kittappa R; Leto K; Gates M; Borel M; Paulsen O; Spitzer S; Karadottir RT; Rossi F; Falk A; Smith A
• Article: STEM CELLS. 2012;30(6):1163-1173

  Treatment of a Mouse Model of Spinal Cord Injury by Transplantation of Human Induced Pluripotent Stem Cell-Derived Long-Term Self-Renewing Neuroepithelial-Like Stem Cells

  Fujimoto Y; Abematsu M; Falk A; Tsujimura K; Sanosaka T; Juliandi B; Semi K; Namihira M; Komiya S; Smith A; Nakashima K
• Article: PLOS ONE. 2012;7(1):e29597

  Capture of Neuroepithelial-Like Stem Cells from Pluripotent Stem Cells Provides a Versatile System for In Vitro Production of Human Neurons

  Falk A; Koch P; Kesavan J; Takashima Y; Ladewig J; Alexander M; Wiskow O; Tailor J; Trotter M; Pollard S; Smith A; Bruestle O

All publications from group members

• Doctoral thesis: 2025

  Modeling early human neural development using iPS cells

  Winn D
• Preprint: BIORXIV. 2025

  Cell type-specific intronic RNAs shape genome architecture during neuronal lineage specification

  Kang W; Yip H; Verron Q; Bouwman BAM; Li-Wang X; Yaghi AA; Murata M; Stout M; Salviati L; Shu X; Yasuzawa K; Gaviraghi M; Pracana R; Lord J; Ballarino R; Falk A; Shin JW; Kasukawa T; Yip CW; Kato M; Takahashi H; Crosetto N; Carninci P; Bienko M
• Preprint: BIORXIV. 2025

  Rapid and efficient generation of human oligodendrocytes myelinating adult human cortical neurons

  Martinez-Curiel R; Rincón-Cerrada P; Al Khani A-M; Tsupykov O; Martín-Hernández D; Monni E; Savchenko E; Rodríguez LR; Kidnapillai S; Bruzelius A; Hidalgo I; Canals I; Bengzon J; Skibo G; Ottosson DR; Falk A; Ahlenius H; Lindvall O; Kokaia Z; Palma-Tortosa S
• Editorial comment: CELL STEM CELL. 2025;32(3):341-342

  Autologous cells, no longer lost in translation.

  Parmar M; Falk A
• Article: COMMUNICATIONS BIOLOGY. 2025;8(1):255

  Defined culture conditions robustly maintain human stem cell pluripotency, highlighting a role for Ca²⁺ signaling

  Eidhof I; Ulfenborg B; Kele M; Shahsavani M; Winn D; Uhlen P; Falk A
• Article: BRAIN BEHAVIOR AND IMMUNITY. 2025;123:28-42

  Bi-allelic NRXN1α deletion in microglia derived from iPSC of an autistic patient increases interleukin-6 production and impairs supporting function on neuronal networking

  Bose R; Posada-Perez M; Karvela E; Skandik M; Keane L; Falk A; Spulber S; Joseph B; Ceccatelli S
• Article: NEURO-ONCOLOGY. 2024;26(9):1685-1699

  High-throughput neural stem cell-based drug screening identifies S6K1 inhibition as a selective vulnerability in sonic hedgehog-medulloblastoma

  Zhou L; van Bree N; Boutin L; Ryu J; Moussaud S; Liu M; Otrocka M; Olsson M; Falk A; Wilhelm M
• Article: ADVANCED SCIENCE. 2024;11(25):e2401859

  On-Chip Neural Induction Boosts Neural Stem Cell Commitment: Toward a Pipeline for iPSC-Based Therapies

  Jain S; Voulgaris D; Thongkorn S; Hesen R; Hagg A; Moslem M; Falk A; Herland A
• Preprint: BIORXIV. 2024

  High-throughput neural stem cell-based drug screening identifies S6K1 inhibition as a selective vulnerability in SHH-medulloblastoma

  Zhou L; van Bree N; Boutin L; Moussaud S; Otrocka M; Falk A; Wilhelm M
• Editorial comment: CELL STEM CELL. 2024;31(2):152-154

  Unraveling the brain's response to COVID-19: How SARS-CoV-2 afflicts dopaminergic neurons

  Eidhof I; Twohig D; Falk A
• Article: FRONTIERS IN PHARMACOLOGY. 2024;15:1390058

  Pre-clinical evaluation of clinically relevant iPS cell derived neuroepithelial stem cells as an off-the-shelf cell therapy for spinal cord injury

  Winn D; Uhlin E; Kele M; Eidhof I; Falk A
• Article: BIOLOGY OPEN. 2023;12(10):bio060113

  Deficiency of the Heterogeneous Nuclear Ribonucleoprotein U locus leads to delayed hindbrain neurogenesis

  Mastropasqua F; Oksanen M; Soldini C; Alatar S; Arora A; Ballarino R; Molinari M; Agostini F; Poulet A; Watts M; Rabkina I; Becker M; Li D; Anderlid B-M; Isaksson J; Remnelius KL; Moslem M; Jacob Y; Falk A; Crosetto N; Bienko M; Santini E; Borgkvist A; Bo''lte S; Tammimies K
• Article: SCIENTIFIC REPORTS. 2023;13(1):10519

  Screening autism-associated environmental factors in differentiating human neural progenitors with fractional factorial design-based transcriptomics

  Arora A; Becker M; Marques C; Oksanen M; Li D; Mastropasqua F; Watts ME; Arora M; Falk A; Daub CO; Lanekoff I; Tammimies K
• Journal article: NEUROSCIENCE APPLIED. 2023;2:101125

  Recommendations, guidelines, and best practice for the use of human induced pluripotent stem cells for neuropharmacological studies of neuropsychiatric disorders

  Polit LD; Eidhof I; McNeill RV; Warre-Cornish KM; Ohki CMY; Walter NM; Sala C; Verpelli C; Radtke F; Galderisi S; Mucci A; Collo G; Edenhofer F; Castrén ML; Réthelyi JM; Ejlersen M; Hohmann SS; Ilieva MS; Lukjanska R; Matuleviciute R; Michel TM; de Vrij FMS; Kushner SA; Lendemeijer B; Kittel-Schneider S; Ziegler GC; Gruber-Schoffnegger D; Pasterkamp RJ; Kasri A; Potier M-C; Knoblich JA; Brüstle O; Peitz M; Pich EM; Harwood AJ; Abranches E; Falk A; Vernon AC; Grünblatt E; Srivastava DP
• Article: HUMAN MUTATION. 2022;43(11):1567-1575

  Multi-omics analysis reveals multiple mechanisms causing Prader-Willi like syndrome in a family with a X;15 translocation

  Eisfeldt J; Rezayee F; Pettersson M; Lagerstedt K; Malmgren H; Falk A; Grigelioniene G; Lindstrand A
• Article: STEM CELL REPORTS. 2022;17(10):2203-2219

  Age-related pathological impairments in directly reprogrammed dopaminergic neurons derived from patients with idiopathic Parkinson's disease

  Drouin-Ouellet J; Legault EM; Nilsson F; Pircs K; Bouquety J; Petit F; Shrigley S; Birtele M; Pereira M; Storm P; Sharma Y; Bruzelius A; Vuono R; Kele M; Stoker TB; Ottosson DR; Falk A; Jakobsson J; Barker RA; Parmar M
• Preprint: BIORXIV. 2022

  Deficiency of the Heterogeneous Nuclear Ribonucleoprotein U locus leads to delayed hindbrain neurogenesis

  Mastropasqua F; Oksanen M; Soldini C; Alatar S; Arora A; Ballarino R; Molinari M; Agostini F; Poulet A; Watts M; Rabkina I; Becker M; Li D; Anderlid B-M; Isaksson J; Remnelius KL; Moslem M; Jacob Y; Falk A; Crosetto N; Bienko M; Santini E; Borgkvist A; Bölte S; Tammimies K
• Article: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2022;23(16):9392

  Multi-Omic Investigations of a 17-19 Translocation Links MINK1 Disruption to Autism, Epilepsy and Osteoporosis

  Eisfeldt J; Schuy J; Stattin E-L; Kvarnung M; Falk A; Feuk L; Lindstrand A
• Article: ENVIRONMENTAL TOXICOLOGY. 2022;37(8):2044-2057

  Glyphosate-based herbicide induces long-lasting impairment in neuronal and glial differentiation

  Reis L; Raciti M; Rodriguez PG; Joseph B; Al Rayyes I; Uhlen P; Falk A; Lima STDC; Ceccatelli S
• Preprint: BIORXIV. 2022

  Screening autism-associated environmental factors in differentiating human neural progenitors with fractional factorial design-based transcriptomics

  Arora A; Becker M; Marques C; Oksanen M; Li D; Mastropasqua F; Watts ME; Arora M; Falk A; Daub CO; Lanekoff I; Tammimies K
• Article: EBIOMEDICINE. 2022;77:103882

  Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia

  Xu T; Li X; Guo Y; Uhlin E; Holmberg L; Mitra S; Winn D; Falk A; Sundstrom E
• Article: STAR PROTOCOLS. 2021;2(2):100528

  Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro

  Calvo-Garrido J; Winn D; Maffezzini C; Wedell A; Freyer C; Falk A; Wredenberg A
• Article: JOURNAL OF NEUROINFLAMMATION. 2021;18(1):124

  Crosstalk between astrocytes and microglia results in increased degradation of α-synuclein and amyloid-β aggregates

  Rostami J; Mothes T; Kolahdouzan M; Eriksson O; Moslem M; Bergstrom J; Ingelsson M; O'Callaghan P; Healy LM; Falk A; Erlandsson A
• Article: NEUROTHERAPEUTICS. 2021;18(2):1257-1272

  Transplantation of Human Neural Precursor Cells Reverses Syrinx Growth in a Rat Model of Post-Traumatic Syringomyelia

  Xu N; Xu T; Mirasol R; Holmberg L; Vincent PH; Li X; Falk A; Benedikz E; Rotstein E; Seiger A; Akesson E; Falci S; Sundstrom E
• Article: FRONTIERS IN GENETICS. 2021;12:803683

  Partial Monosomy 21 Mirrors Gene Expression of Trisomy 21 in a Patient-Derived Neuroepithelial Stem Cell Model

  Schuy J; Eisfeldt J; Pettersson M; Shahrokhshahi N; Moslem M; Nilsson D; Dahl N; Shahsavani M; Falk A; Lindstrand A
• Corrigendum: BIOMEDICINE & PHARMACOTHERAPY. 2021;133:111019

  Prolyl oligopeptidase inhibition by KYP-2407 increases alpha-synuclein fibril degradation in neuron-like cells (vol 131, 110788, 2020)

  Rostami J; Jantti M; Cui H; Rinne MK; Kukkonen JP; Falk A; Erlandsson A; Myohanen T
• Conference publication: GLIA. 2021;69:E186

  Crosstalk between human iPSC-derived astrocytes and neurons in a cell culture model of Alzheimer's disease

  Konstantinidis E; Beretta C; Falk A; Erlandsson A
• Article: VIRUSES-BASEL. 2020;13(1):E37-37

  Broadly Active Antiviral Compounds Disturb Zika Virus Progeny Release Rescuing Virus-Induced Toxicity in Brain Organoids

  Pettke A; Tampere M; Pronk R; Wallner O; Falk A; Warpman Berglund U; Helleday T; Mirazimi A; Puumalainen M-R
• Article: BIOMEDICINE & PHARMACOTHERAPY. 2020;131:110788

  Prolyl oligopeptidase inhibition by KYP-2407 increases alpha-synuclein fibril degradation in neuron-like cells

  Rostami J; Jantti M; Cui H; Rinne MK; Kukkonen JP; Falk A; Erlandsson A; Myohanen T
• Article: TRANSLATIONAL PSYCHIATRY. 2020;10(1):312

  Presynaptic dysfunction inCASK-related neurodevelopmental disorders

  Becker M; Mastropasqua F; Reising JP; Maier S; Ho M-L; Rabkina I; Li D; Neufeld J; Ballenberger L; Myers L; Moritz V; Kele M; Wincent J; Willfors C; Sitnikov R; Herlenius E; Anderlid B-M; Falk A; Bolte S; Tammimies K
• Review: MOLECULAR AND CELLULAR NEUROSCIENCE. 2020;107:103533

  Models of the blood-brain barrier using iPSC-derived cells

  Delsing L; Herland A; Falk A; Hicks R; Synnergren J; Zetterberg H
• Article: NATURE BIOMEDICAL ENGINEERING. 2020;4(9):875-888

  Three-dimensional single-cell imaging for the analysis of RNA and protein expression in intact tumour biopsies

  Tanaka N; Kanatani S; Kaczynska D; Fukumoto K; Louhivuori L; Mizutani T; Kopper O; Kronqvist P; Robertson S; Lindh C; Kis L; Pronk R; Niwa N; Matsumoto K; Oya M; Miyakawa A; Falk A; Hartman J; Sahlgren C; Clevers H; Uhlen P
• Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2020;117(33):20127-20138

  Modeling SHH-driven medulloblastoma with patient iPS cell-derived neural stem cells

  Susanto E; Navarro AM; Zhou L; Sundstrom A; van Bree N; Stantic M; Moslem M; Tailor J; Rietdijk J; Zubillaga V; Huebner J-M; Weishaupt H; Wolfsberger J; Alafuzoff I; Nordgren A; Magnaldo T; Siesjo P; Johnsen JI; Kool M; Tammimies K; Darabi A; Swartling FJ; Falk A; Wilhelm M
• Corrigendum: NATURE COMMUNICATIONS. 2020;11(1):3504

  Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells (vol 11, 1609, 2020)

  Reyes AP; Petrus-Reurer S; Sanchez SP; Kumar P; Douagi I; Bartuma H; Aronsson M; Westman S; Lardner E; Andre H; Falk A; Nandrot EF; Kvanta A; Lanner F
• Article: MOLECULAR NEUROBIOLOGY. 2020;57(7):2944-2958

  Dyslexia Candidate Gene and Ciliary Gene Expression Dynamics During Human Neuronal Differentiation

  Bieder A; Yoshihara M; Katayama S; Krjutskov K; Falk A; Kere J; Tapia-Paez I
• Review: MOLECULAR AUTISM. 2020;11(1):42

  Copy number variants (CNVs): a powerful tool for iPSC-based modelling of ASD

  Drakulic D; Djurovic S; Syed YA; Trattaro S; Caporale N; Falk A; Ofir R; Heine VM; Chawner SJRA; Rodriguez-Moreno A; van den Bree MBM; Testa G; Petrakis S; Harwood AJ
• Article: BIOMATERIALS SCIENCE. 2020;8(9):2514-2525

  Assembly of FN-silk with laminin-521 to integrate hPSCs into a three-dimensional culture for neural differentiation

  Astrand C; Chotteau V; Falk A; Hedhammar M
• Corrigendum: NATURE COMMUNICATIONS. 2020;11(1):2346

  Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells (vol 11, 1609, 2020)

  Reyes AP; Petrus-Reurer S; Sanchez SP; Kumar P; Douagi I; Bartuma H; Aronsson M; Westman S; Lardner E; Andre H; Falk A; Nandrot EF; Kvanta A; Lanner F
• Article: ADVANCED BIOLOGY. 2020;4(5):e1900226

  hiPS-Derived Astroglia Model Shows Temporal Transcriptomic Profile Related to Human Neural Development and Glia Competence Acquisition of a Maturing Astrocytic Identity

  Lundin A; Ricchiuto P; Clausen M; Hicks R; Falk A; Herland A
• Article: NATURE COMMUNICATIONS. 2020;11(1):1609

  Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells

  Reyes AP; Petrus-Reurer S; Sanchez SP; Kumar P; Douagi I; Bartuma H; Aronsson M; Westman S; Lardner E; Andre H; Falk A; Nandrot EF; Kvanta A; Lanner F
• Article: CELL DEATH & DISEASE. 2020;11(1):52

  p53 controls genomic stability and temporal differentiation of human neural stem cells and affects neural organization in human brain organoids

  Navarro AM; Pronk RJ; van der Geest AT; Oliynyk G; Nordgren A; Arsenian-Henriksson M; Falk A; Wilhelm M
• Article: CLINICAL EPIGENETICS. 2020;12(1):9

  DNA methylation changes in Down syndrome derived neural iPSCs uncover co-dysregulation of ZNF and HOX3 families of transcription factors

  Laan L; Klar J; Sobol M; Hoeber J; Shahsavani M; Kele M; Fatima A; Zakaria M; Anneren G; Falk A; Schuster J; Dahl N
• Conference publication: EUROPEAN JOURNAL OF HUMAN GENETICS. 2020;28(SUPPL 1):588

  A case of ring chromosome 21 with hemifacial microsomia, developmental and speech delay analysed with WGS and patient-derived iPS

  Schuy J; Eisfeldt J; Pettersson M; Shahrokhshahi N; Shahsavani M; Nilsson D; Falk A; Lindstrand A
• Article: CELL STEM CELL. 2019;25(6):855-870.e11

  Humanized Stem Cell Models of Pediatric Medulloblastoma Reveal an Oct4/mTOR Axis that Promotes Malignancy

  Cancer M; Hutter S; Holmberg KO; Rosen G; Sundstrom A; Tailor J; Bergstrom T; Garancher A; Essand M; Wechsler-Reya RJ; Falk A; Weishaupt H; Swartling FJ
• Article: GENES TO CELLS. 2019;24(12):836-847

  Single-cell study of neural stem cells derived from human iPSCs reveals distinct progenitor populations with neurogenic and gliogenic potential

  Lam M; Sanosaka T; Lundin A; Imaizumi K; Etal D; Karlsson FH; Clausen M; Cairns J; Hicks R; Kohyama J; Kele M; Okano H; Falk A
• Review: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY. 2019;2019:2149812-10

  Oxidative DNA Damage Signalling in Neural Stem Cells in Alzheimer's Disease.

  Kieroń M; Żekanowski C; Falk A; Wężyk M
• Article: MOLECULAR NEUROBIOLOGY. 2019;56(10):7113-7127

  Transcriptome and Proteome Profiling of Neural Induced Pluripotent Stem Cells from Individuals with Down Syndrome Disclose Dynamic Dysregulations of Key Pathways and Cellular Functions

  Sobol M; Klar J; Laan L; Shahsavani M; Schuster J; Anneren G; Konzer A; Mi J; Bergquist J; Nordlund J; Hoeber J; Huss M; Falk A; Dahl N
• Article: EXPERIMENTAL CELL RESEARCH. 2019;383(1):111469

  Single cell analysis of autism patient with bi-allelic NRXN1-alpha deletion reveals skewed fate choice in neural progenitors and impaired neuronal functionality

  Lam M; Moslem M; Bryois J; Pronk RJ; Uhlin E; Ellstrom ID; Laan L; Olive J; Morse R; Ronnholm H; Louhivuori L; Korol SV; Dahl N; Uhlen P; Anderlid B-M; Kele M; Sullivan PF; Falk A
• Review: SCHIZOPHRENIA RESEARCH. 2019;210:3-12

  Stem cell models of schizophrenia, what have we learned and what is the potential?

  Moslem M; Olive J; Falk A
• Article: MOLECULAR GENETICS & GENOMIC MEDICINE. 2019;7(6):e654

  Mutations in the mitochondrial tryptophanyl-tRNA synthetase cause growth retardation and progressive leukoencephalopathy

  Maffezzini C; Laine I; Dallabona C; Clemente P; Calvo-Garrido J; Wibom R; Naess K; Barbaro M; Falk A; Donnini C; Freyer C; Wredenberg A; Wedell A
• Article: JOURNAL OF MOLECULAR CELL BIOLOGY. 2019;11(5):356-370

  Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification

  Heras G; Namuduri AV; Traini L; Shevchenko G; Falk A; Lind SB; Jia M; Tian G; Gastaldello S
• Article: STEM CELL REPORTS. 2019;12(4):696-711

  SQSTM1/p62-Directed Metabolic Reprogramming Is Essential for Normal Neurodifferentiation

  Calvo-Garrido J; Maffezzini C; Schober FA; Clemente P; Uhlin E; Kele M; Stranneheim H; Lesko N; Bruhn H; Svenningsson P; Falk A; Wedell A; Freyer C; Wredenberg A
• Article: NEUROSCIENCE. 2019;402:78-89

  The T-type Ca²⁺ Channel Ca_v3.2 Regulates Differentiation of Neural Progenitor Cells during Cortical Development via Caspase-3

  Rebellato P; Kaczynska A; Kanatani S; Al Rayyes I; Zhang S; Villaescusa C; Falk A; Arenas E; Hermanson O; Louhivuori L; Uhlen P
• Article: FRONTIERS IN GENETICS. 2019;10:896

  Ataxia in Patients With Bi-Allelic NFASC Mutations and Absence of Full-Length NF186

  Kvarnung M; Shahsavani M; Taylan F; Moslem M; Breeuwsma N; Laan L; Schuster J; Jin Z; Nilsson D; Lieden A; Anderlid B-M; Nordenskjold M; Lundberg ES; Birnir B; Dahl N; Nordgren A; Lindstrand A; Falk A
• Article: STEM CELL RESEARCH. 2019;34:101356

  Generation of induced pluripotent stem cell lines from two Neuroblastoma patients carrying a germline ALK R1275Q mutation

  Navarro AM; Day K; Kogner P; Wilhelm M; Falk A
• Article: FRONTIERS IN GENETICS. 2019;10:593

  NRXN1 Deletion and Exposure to Methylmercury Increase Astrocyte Differentiation by Different Notch-Dependent Transcriptional Mechanisms

  Raciti M; Selma J; Spulber S; Gaudenzi G; Khalajzeyqami Z; Conti M; Anderlid B-M; Falk A; Hermanson O; Ceccatelli S
• Conference publication: EUROPEAN JOURNAL OF HUMAN GENETICS. 2019;27:1143

  Absence of NFASC isoform NF186 causes an autosomal recessive ataxia syndrome

  Kvarnung M; Shahsavani M; Taylan F; Breeuwsma N; Laan L; Schuster J; Jin Z; Nilsson D; Lieden A; Anderlid B; Nordenskjold M; Lundberg ES; Birnir B; Dahl N; Nordgren A; Lindstrand A; Falk A
• Conference publication: EUROPEAN JOURNAL OF HUMAN GENETICS. 2019;27:1437

  Understanding the role of HNRNPU in brain development and neurodevelopmental disorders

  Mastropasqua F; Rabkina I; Becker M; Anderlid B; Bolte S; Falk A; Tammimies K
• Conference publication: EUROPEAN JOURNAL OF HUMAN GENETICS. 2019;27:1501-1502

  Down syndrome-iPSC neurogenesis connects differential methylation to dysregulated gene expression

  Laan L; Klar J; Sobol M; Hoeber J; Zakaria M; Anneren G; Falk A; Schuster J; Dahl N
• Review: CELL DEATH DISCOVERY. 2018;4:7

  Modeling cancer using patient-derived induced pluripotent stem cells to understand development of childhood malignancies

  Navarro AM; Susanto E; Falk A; Wilhelm M
• Article: STEM CELLS. 2018;36(12):1816-1827

  Barrier Properties and Transcriptome Expression in Human iPSC-Derived Models of the Blood-Brain Barrier

  Delsing L; Donnes P; Sanchez J; Clausen M; Voulgaris D; Falk A; Herland A; Brolen G; Zetterberg H; Hicks R; Synnergren J
• Editorial comment: STEM CELL REPORTS. 2018;11(4):847-849

  Quick Access to Human Astrocytic Software that Drives Neuronal Hardware

  Lundin A; Falk A
• Article: MOLECULAR PSYCHIATRY. 2018;23(7):1674-1684

  An in vitro model of lissencephaly: expanding the role of DCX during neurogenesis

  Shahsavani M; Pronk RJ; Falk R; Lam M; Moslem M; Linker SB; Salma J; Day K; Schuster J; Anderlid B-M; Dahl N; Gage FH; Falk A
• Article: STEM CELL REPORTS. 2018;10(3):1030-1045

  Human iPS-Derived Astroglia from a Stable Neural Precursor State Show Improved Functionality Compared with Conventional Astrocytic Models

  Lundin A; Delsing L; Clausen M; Ricchiuto P; Sanchez J; Sabirsh A; Ding M; Synnergren J; Zetterberg H; Brolen G; Hicks R; Herland A; Falk A
• Article: JOURNAL OF ALZHEIMERS DISEASE. 2018;62(1):175-202

  Overactive BRCA1 Affects Presenilin 1 in Induced Pluripotent Stem Cell-Derived Neurons in Alzheimer's Disease

  Wezyk M; Szybinska A; Wojsiat J; Szczerba M; Day K; Ronnholm H; Kele M; Berdynski M; Peplonska B; Fichna JP; Ilkowski J; Styczynska M; Barczak A; Zboch M; Filipek-Gliszczynska A; Bojakowski K; Skrzypczak M; Ginalski K; Kabza M; Makalowska I; Barcikowska-Kotowicz M; Wojda U; Falk A; Zekanowski C
• Conference publication: EUROPEAN JOURNAL OF HUMAN GENETICS. 2018;26:366

  Modeling early neuropathology in Down syndrome and APP-associated Alzheimer's disease using Induced Pluripotent Stem Cells

  Klar J; Schuster J; Laan L; Sobol M; Lorenzo LP; Nordlund J; Shahsavani M; Falk A; Dahl N
• Journal article: NEURO-ONCOLOGY. 2017;19(suppl_6):vi255

  TMOD-05. MYCN OVEREXPRESSION AND STABILIZATION DRIVES MEDULLOBLASTOMA FROM HUMAN NEURO-EPITHELIAL STEM CELLS

  Čančer M; Hutter S; Rosén G; Hellström A-C; Essand M; Huang M; Tailor J; Smith A; Weiss WA; Falk A; Weishaupt H; Swartling F
• Article: SCIENTIFIC REPORTS. 2017;7(1):11458

  Acute doses of caffeine shift nervous system cell expression profiles toward promotion of neuronal projection growth

  Yu NY; Bieder A; Raman A; Mileti E; Katayama S; Einarsdottir E; Fredholm BB; Falk A; Tapia-Paez I; Daub CO; Kere J
• Journal article: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS. 2017;(125)

  Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

  Uhlin E; Marin Navarro A; Rönnholm H; Day K; Kele M; Falk A
• Article: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS. 2017;(125)

  Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

  Uhlin E; Navarro AM; Ronnholm H; Day K; Kele M; Falk A
• Article: CELL REPORTS. 2017;18(1):1-11

  TRIM28 Controls a Gene Regulatory Network Based on Endogenous Retroviruses in Human Neural Progenitor Cells

  Brattas PL; Jonsson ME; Fasching L; Wahlestedt JN; Shahsavani M; Falk R; Falk A; Jern P; Parmar M; Jakobsson J
• Article: STEM CELL RESEARCH. 2017;18:22-25

  Derivation of human iPS cell lines from monozygotic twins in defined and xeno free conditions

  Uhlin E; Ronnholm H; Day K; Kele M; Tammimies K; Bolte S; Falk A
• Editorial comment: STEM CELL RESEARCH. 2016;17(3):474-478

  Generation of human iPS cell line CTL07-II from human fibroblasts, under defined and xeno-free conditions

  Kele M; Day K; Ronnholm H; Schuster J; Dahl N; Falk A
• Article: EMBO JOURNAL. 2016;35(18):1963-1978

  A PBX1 transcriptional network controls dopaminergic neuron development and is impaired in Parkinson's disease

  Villaescusa JC; Li B; Toledo EM; Cervo PRDV; Yang S; Stott SRW; Kaiser K; Islam S; Gyllborg D; Laguna-Goya R; Landreh M; Lonnerberg P; Falk A; Bergman T; Barker RA; Linnarsson S; Selleri L; Arenas E
• Corrigendum: MOLECULAR PSYCHIATRY. 2016;21(9):1321

  Modeling psychiatric disorders: from genomic findings to cellular phenotypes.

  Falk A; Heine VM; Harwood AJ; Sullivan PF; Peitz M; Brüstle O; Shen S; Sun Y-M; Glover JC; Posthuma D; Djurovic S
• Review: MOLECULAR PSYCHIATRY. 2016;21(9):1167-1179

  Modeling psychiatric disorders: from genomic findings to cellular phenotypes

  Falk A; Heine VM; Harwood AJ; Sullivan PF; Peitz M; Bruestle O; Shen S; Sun Y-M; Glover JC; Posthuma D; Djurovic S
• Article: NEUROPHARMACOLOGY. 2016;107:422-431

  Glucocorticoids alter neuronal differentiation of human neuroepithelial-like cells by inducing long-lasting changes in the reactive oxygen species balance

  Raciti M; Ong J; Weis L; Edoff K; Battagli C; Falk A; Ceccatelli S
• Article: AMERICAN JOURNAL OF STEM CELLS. 2016;5(1):19-28

  Induction of sensory neurons from neuroepithelial stem cells by the ISX9 small molecule

  Ali RQ; Blomberg E; Falk A; Ahrlund-Richter L; Ulfendahl M
• Article: BIOMATERIALS. 2014;35(30):8496-8502

  Spider silk for xeno-free long-term self-renewal and differentiation of human pluripotent stem cells

  Wu S; Johansson J; Damdimopoulou P; Shahsavani M; Falk A; Hovatta O; Rising A
• Article: TWIN RESEARCH AND HUMAN GENETICS. 2014;17(3):164-176

  The Roots of Autism and ADHD Twin Study in Sweden (RATSS).

  Bölte S; Willfors C; Berggren S; Norberg J; Poltrago L; Mevel K; Coco C; Fransson P; Borg J; Sitnikov R; Toro R; Tammimies K; Anderlid B-M; Nordgren A; Falk A; Meyer U; Kere J; Landén M; Dalman C; Ronald A; Anckarsäter H; Lichtenstein P
• Article: BIOMATERIALS. 2014;35(5):1420-1428

  A 3D Alzheimer's disease culture model and the induction of P21-activated kinase mediated sensing in iPSC derived neurons

  Zhang D; Pekkanen-Mattila M; Shahsavani MO; Falk A; Teixeira AI; Herland A
• Article: JOURNAL OF NEUROSCIENCE. 2013;33(30):12407-12422

  Stem Cells Expanded from the Human Embryonic Hindbrain Stably Retain Regional Specification and High Neurogenic Potency

  Tailor J; Kittappa R; Leto K; Gates M; Borel M; Paulsen O; Spitzer S; Karadottir RT; Rossi F; Falk A; Smith A
• Article: JOURNAL OF BIOMOLECULAR SCREENING. 2013;18(3):258-268

  Automated Large-Scale Culture and Medium-Throughput Chemical Screen for Modulators of Proliferation and Viability of Human Induced Pluripotent Stem Cell-Derived Neuroepithelial-like Stem Cells

  McLaren D; Gorba T; de Rotrou AM; Pillai G; Chappell C; Stacey A; Lingard S; Falk A; Smith A; Koch P; Bruestle O; Vickers R; Tinsley J; Flanders D; Bello P; Craig S
• Article: METHODS. 2012;58(1):69-78

  Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells

  Falk R; Falk A; Dyson MR; Melidoni AN; Parthiban K; Young JL; Roake W; McCafferty J
• Article: STEM CELLS. 2012;30(6):1163-1173

  Treatment of a Mouse Model of Spinal Cord Injury by Transplantation of Human Induced Pluripotent Stem Cell-Derived Long-Term Self-Renewing Neuroepithelial-Like Stem Cells

  Fujimoto Y; Abematsu M; Falk A; Tsujimura K; Sanosaka T; Juliandi B; Semi K; Namihira M; Komiya S; Smith A; Nakashima K
• Article: PLOS ONE. 2012;7(1):e29597

  Capture of Neuroepithelial-Like Stem Cells from Pluripotent Stem Cells Provides a Versatile System for In Vitro Production of Human Neurons

  Falk A; Koch P; Kesavan J; Takashima Y; Ladewig J; Alexander M; Wiskow O; Tailor J; Trotter M; Pollard S; Smith A; Bruestle O
• Article: NEUROCHEMISTRY INTERNATIONAL. 2011;59(3):432-444

  Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays

  Hook L; Vives J; Fulton N; Leveridge M; Lingard S; Bootman MD; Falk A; Pollard SM; Allsopp TE; Dalma-Weiszhausz D; Tsukamoto A; Uchida N; Gorba T
• Article: BIOCHEMICAL SOCIETY TRANSACTIONS. 2010;38(4):1067-1071

  Imaging-based chemical screens using normal and glioma-derived neural stem cells

  Danovi D; Falk A; Humphreys P; Vickers R; Tinsley J; Smith AG; Pollard SM
• Article: PLOS ONE. 2009;4(5):e5498

  CD133 (Prominin) Negative Human Neural Stem Cells Are Clonogenic and Tripotent

  Sun Y; Kong W; Falk A; Hu J; Zhou L; Pollard S; Smith A
• Article: MOLECULAR AND CELLULAR NEUROSCIENCE. 2008;38(2):245-258

  Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture

  Sun Y; Pollard S; Conti L; Toselli M; Biella G; Parkin G; Willatt L; Falk A; Cattaneo E; Smith A
• Article: STEM CELLS. 2007;25(6):1539-1545

  High-throughput identification of genes promoting neuron formation and lineage choice in mouse embryonic stem cells

  Falk A; Karlsson TE; Kurdija S; Frisen J; Zupicich J
• Review: ANNALS OF MEDICINE. 2005;37(7):480-486

  New neurons in old brains

  Falk A; Frisén J
• Article: DEVELOPMENT. 2003;130(24):6089-6099

  Functional notch signaling is required for BMP4-induced inhibition of myogenic differentiation

  Dahlqvist C; Blokzijl A; Chapman G; Falk A; Dannaeus K; Ibâñez CF; Lendahl U
• Article: JOURNAL OF CELL BIOLOGY. 2003;163(4):723-728

  Cross-talk between the Notch and TGF-β signaling pathways mediated by interaction of the Notch intracellular domain with Smad3

  Blokzijl A; Dahlqvist C; Reissmann E; Falk A; Moliner A; Lendahl U; Ibáñez CF
• Article: JOURNAL OF NEUROSCIENCE RESEARCH. 2002;69(6):757-762

  Amphiregulin is a mitogen for adult neural stem cells

  Falk A; Frisén J
• Article: EXPERIMENTAL CELL RESEARCH. 2002;279(1):34-39

  Gene delivery to adult neural stem cells

  Falk A; Holmström N; Carlén M; Cassidy R; Lundberg C; Frisén J
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Staff and contact

Group leader

• 

  Anna Falk

  Principal Researcher | Docent

  Telephone +46852482897

  E-mail anna.falk@ki.se

All members of the group

• 

  Anna Falk

  Principal Researcher | Docent

  Telephone +46852482897

  E-mail anna.falk@ki.se
• Daniel Twohig

  Affiliated to Research

  E-mail daniel.twohig.2@ki.se
• Dania Winn

  Phd Student | Research Assistant

  E-mail dania.winn@ki.se

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