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Robert Månsson group

The hematopoietic system is maintained through the continuous generation of new mature effector cells from self-renewing multipotent progenitors. A major challenge for the field is to understand mechanisms behind this differentiation process.

Our research mainly focuses around the developmental progression from stem and progenitor cells into mature B-lymphoid cells and the transcriptional networks that drive this process. We study this through a combination of in vivo models and cell- and molecular biology techniques.

Overall aim is to understand the differentiation process and through this increase our understanding of diseases that originate from developmental defects such as cancer and various immunodeficiencies.

Keywords: hematopoietic progenitor, B-cell development, transcriptional network, transcription factor, differentiation, interacting regulatory elements

Members

Robert Månsson, Group Leader, Assistant Professor.

He received his PhD in Stem Cell Biology from the Faculty of Medicine at Lund University in 2007. He then continued his research as a post doctorial fellow at University of California, San Diego. Since early 2012 he holds an assistant professor position at Karolinska Institutet. Phone: +46 (0)8 - 585 8623

Charlotte Gustafsson, PhD, Senior Lab Manager.

She received her PhD in Clinical Immunology from the Faculty of Health Sciences at Linköping University in 2007. Before starting at Karolinska Institutet in september 2012, she worked as a Research Scientist at AstraZeneca in Södertälje. Phone: +46 (0)8 - 585 826 76

Shabnam Kharazi, Postdoc.

She got her PhD in Stem Cell Biology from the Faculty of Medicine at Lund University in 2010. She continued as a postdoc in Pasteur Institute of Iran and then started her second postoc at Karolinska Institutet. Phone: +46 (08)- 585 83623

Xiaoze Li, PhD, Postdoc.

She received her PhD in Virology and Microbiology from the Faculty of Medicine at Lund University in 2013. She started her postdoc at Karolinska Institutet in January 2014. Phone: +46(08)-58583623.

Ayla De Paepe, doctoral student.

She graduated as a Master of Science in Bioscience Engineering from Ghent University, Belgium. She started as a doctoral student at Karolinska Institutet in January 2014. Phone: +46 (0)7 - 627 734 31

Aleksandra Krstic, PhD, Postdoc.

She received her PhD in Molecular Medicine from the Faculty of Medicine at Belgrade University in 2012. She started her postdoc at Karolinska Institute in March 2013. Phone: +46(08)-58583623.

Projects

The hematopoietic system is maintained through the continuous generation of new mature effector cells from self-renewing multipotent progenitors. Development of mature cells occur gradually through series of hierarchically related developmental stages.

This gradual process coupled with the relative ease by which different developmental stages can be purified (based on stage and lineage specific surface markers) make hematopoiesis an ideal system for studying developmental progression. In particular the development of B-cells has been very well characterized leading to the identification of several successive stages of B-cell development. In addition a hierarchically organized network of transcription factors has been identified. This network includes several transcription factors (EBF1, E2A, PAX5, FOXO1 and others), all demonstrated to be critical for proper development and commitment to the B-cell lineage. Together this makes the development of B-cell possibly the most well understood developmental pathways to date.

However, even though the developmental pathway is well defined, our understanding of the molecular mechanisms that drive differentiation and lineage specification is still limited. Thus, a major challenge for the field is to understand how elaborate transcriptional programs drive these processes through temporal and cell-type specific gene expression.

Studies will explore the developmental progression from stem and progenitor cells into mature B-lymphoid cells and the transcriptional networks that drive this process.

More specifically, studies will be aimed at:

  1. Increasing our ability to resolve discrete stages of developmental progression.
  2. Using high resolution cell separation, coupled to genome wide approaches, to study changes in transcription factor usage with resulting effects on epigenetic marks and transcription in association with developmental progression.
  3. Identification and study of distal regulatory elements and how these interact with and modulate their transcriptional targets.

Financial support

  • Wallenberg Institute for Regenerative Medicine (WIRM)
  • Swedish Research Council (VR)

Selected publications

21969581

21972416

20543837

19996414

19878871

18714008

18674933

18495958

18394553

18371379

17374609

17429843

17433729

15851035

14960572

Open positions

We always want to get in touch with talented potential co-workers. If you are interested in doing research within our group, as a degree project or as a researcher, please contact the group leader Robert Månsson.

Links

HOMER - Software for motif discovery and next generation sequencing analysis

The fantastic next generation sequencing analysis package HOMER developed by Dr. Chris Benner at UCSD. Unfamiliar with sequencing analysis? Fear not, Chuck has been helping Chris make one of the few instruction manuals that you actually want to read!

HOMER