Jaakko Patrakka group

Renal glomerulus biology and diseases

Glomerular diseases are the most common cause of end-stage renal disease. We investigate the molecular profile of healthy and diseased glomeruli with the aim to discover molecular pathways that drive the progression of glomerular disorders. We use transgenic mouse and zebrafish models to understand the role of identified pathways in the biology and diseases of the kidney.

Research group leader

Jaakko Patrakka

Researcher
Patrakka
H5 Department of Laboratory Medicine

Group members

Financial support

  • KI/AZ Integrated Cardio Metabolic Center
  • Marianne and Marcus Wallenberg Foundation
  • Swedish Research Council
  • Swedish Diabetes Foundation
  • Swedish Juvenile Diabetes Foundation
  • KI Foundations
  • Westmans Foundation

Research techniques

  • Generation and characterization of transgenic mouse lines
  • Morpholino and CRISPR/Cas-9 technology in zebrafish. Phenotypic analysis of zebrafish.
  • Cell culture
  • RNA sequencing
  • Expressional profiling by RNAscope, qPCR, immunohistochemistry, Western blot

Selected publications

Formate assay in body fluids: application in methanol poisoning.Delineation of the intimate details of the backbone conformation of pyridine nucleotide coenzymes in aqueous solution.
Makar AB, McMartin KE, Palese M, Tephly TR, Bose KS, Sarma RH
Biochem MedBiochem. Biophys. Res. Commun. 19751975 JunOct;1366(24):117-261173-9

Bovine liver dihydrofolate reductase: purification and properties of the enzyme.
Peterson DL, Gleisner JM, Blakley RL
Biochemistry 1975 Dec;14(24):5261-7

Neuronal proteins are novel components of podocyte major processes and their expression in glomerular crescents supports their role in crescent formation.
Sistani L, Rodriguez PQ, Hultenby K, Uhlen M, Betsholtz C, Jalanko H, et al
Kidney Int. 2013 Jan;83(1):63-71

Pdlim2 is a novel actin-regulating protein of podocyte foot processes.
Sistani L, Dunér F, Udumala S, Hultenby K, Uhlen M, Betsholtz C, et al
Kidney Int. 2011 Nov;80(10):1045-54

New insights into the role of podocytes in proteinuria.
Patrakka J, Tryggvason K
Nat Rev Nephrol 2009 Aug;5(8):463-8

Expression and subcellular distribution of novel glomerulus-associated proteins dendrin, ehd3, sh2d4a, plekhh2, and 2310066E14Rik.
Patrakka J, Xiao Z, Nukui M, Takemoto M, He L, Oddsson A, et al
J. Am. Soc. Nephrol. 2007 Mar;18(3):689-97

Hereditary proteinuria syndromes and mechanisms of proteinuria.
Tryggvason K, Patrakka J, Wartiovaara J
N. Engl. J. Med. 2006 Mar;354(13):1387-401

Proteinuria and prenatal diagnosis of congenital nephrosis in fetal carriers of nephrin gene mutations.
Patrakka J, Martin P, Salonen R, Kestilä M, Ruotsalainen V, Männikkö M, et al
Lancet 2002 May;359(9317):1575-7