Research projects- Germ cell biology, developmental programming and epigenetic inheritance of disease
While specific projects may change over time, our dedication to the following research fields remains constant.
Interestingly, two waves of epigenetic remodeling occur after fertilization to ensure the totipotency of the blastocyst for somatic lineage specification and to ascertain “clean slate” of germ cells for erasing any potentially harmful epigenetic modifications acquired by parents. The evolutionary advantage of these processes is to enable the organism to adapt to changing environmental conditions and minimize the risk of epigenetic inheritance of harmful traits. However, the degree of completeness and faithfulness of these processes still remain to be investigated. More and more studies have shown that parental health condition predisposes their offspring to develop diseases such as obesity, diabetes, cardiovascular disease, and behavioral disorders, which contributes to increased prevalence of chronic diseases. This process is referred to as developmental programming and epigenetic inheritance of diseases. Despite of increasing amount of epidemiological evidence, mechanistic understanding is still sparse. Here, we aim to answer (1) how parental health condition affects the germline that further transmit phenotypic traits? (2) how placenta responds to adverse uterine environment that in turn systematically modify cellular and physiological function of the offspring? (3) can we systematically model maternal disease signature with offspring key organ signature?
We are among those pioneers to apply and develop single-cell RNA sequencing (Smart-seq, Smart-seq2, LCM-seq etc). More tools to answer all these interesting questions are mouse disease models, human iPSC culture and differentiation, organoid culture, human sample cohorts and registry data together with other key cellular and molecular assays.
Research filed I: Develop culture platform to differentiate germ cells towards maturation with epigenetic remodeling for the purpose of disease modeling and mechanistic understanding
The use of pluripotent stem cells for in vitro differentiation provides a most valuable approach to study human germ cell specification and to uncover genetic and epigenetic dysregulations associated with infertility. Our previous and recent work has demonstrated the establishment of formative pluripotency conditions for direct specification of primordial germ cell-like cells (PGCLCs) (Cheng et.al Cell Reports 2019, Luo et.al Cell Reports 2023). Building on this work, we plan to further develop in vitro differentiation platform using organoid co-culture to investigate the impact of genetic mutations on fertility.
Specifically, our research has shown focus on understanding the role of X-chromosome activity and mitochondrial dynamics in germ cell development.
Research field II: Characterize the spatiotemporal regulation of germ cell migration and reveal the mechanisms of quality selection for gametogenesis
Germ cell migration is accompanied by extensive DNA demethylation and histone protein modification. We still know little about how these two sophisticated processes are coordinated. Furthermore, majority of migrating germ cells undergo apoptosis without further commitment to gametogenesis. We aim to use genetic mouse models, lineage tracing, single-cell sequencing among others to reveal gene function and mitochondrial dynamics during migration, epigenetic resetting, and meiosis entry. Especially, we are focusing on human genetic mutations implicated in infertility/subfertility.
Research field III: Investigate the role of germline transmission in epigenetic inheritance of diseases
Women are born with a pool of primary follicles, some of which are periodically matured for fertilization. Throughout a woman's reproductive life, the number and quality of the remaining follicles gradually decline, leading to a decrease in fertility and an increased risk of reproductive disorders. Besides genetics, environmental factors can affect the health and function of the oocytes through dynamic interaction with niche cells. We are particularly interested in how maternal endocrine diseases such as polycystic ovarian syndrome (PCOS) and type I diabetes impact transcriptome and metabolism of oocytes, which in turn affects their offspring health across generations independent of uterine environmental effects. We are using diseased mouse models, IVF and surrogacy, and single-cell sequencing together with phenotyping and molecular assays.
Research field IV: Investigate the molecular basis of maternal-fetal communication in maternal endocrine diseases and their effects on offspring future health
Functional placentation and endometrial receptivity are essential to maintain a healthy pregnancy and influence fetal development. We have previously showed that reproductive and metabolic traits of maternal PCOS can be transmitted across generations (Risal, Pei et.al. Nature Medicine 2019). To further delineate the role of adverse uterine environment versus germline transmission, we are carrying on several projects to study the role of the placenta in developmental programming and offspring’s future health. As increasing evidence has shown that the placenta is not functioned as a passive barrier, instead actively responds and adapts to uterine environment to impact fetal development. It is important to elucidate molecular adaptation of placentas in maternal endocrine diseases in function to maternal clinical features including hormone profiles. We also aim to mediate the placental function and prevent the developmental programming. To address these questions, mouse disease models, single-cell sequencing of human placentas as well placental organoids based chemical screening are applied to systematically examine molecular and phenotypic traits of mother, placentas and offspring and build molecular link underlying the developmental programming effects.