Christer Höög's Group
By describing the function of a protein structure called the synaptonemal complex in germ cells, we hope to get a better understanding of the defective cellular mechanisms that give rise to human eggs with too few or too many chromosomes. The results should contribute to the development of more effective methods for in vitro fertilization.
Meiosis is a specialized cell division process that is active in germ cells. During meiosis, genetically distinct haploid cells are generated through a process that involves one DNA replication step followed by two cell divisions. Meiosis in human female germ cells is highly error-prone and frequently give rise to gametes that have too many or too few chromosomes (aneuploid cells). Aneuploidy is the most common chromosome abnormality in humans and clinically very important, being the leading genetic cause of miscarriage, congenital birth defects and mental retardation.
The focus of the research in the laboratory is to understand the role of the synaptonemal complex. The synaptonemal complex is an evolutionary conserved meiosis-specific protein complex that ensures the fidelity of the chromosome segregation process in germ cells. Despite the central biological role of the synaptonemal complex in meiotic chromosome architecture, our knowledge of the function of this protein complex is yet limited.
To unravel the function of the synaptonemal complex, we are analyzing this structure by a combination of genetic, biochemical and ultrastructural approaches. Inactivation of one of the components of the synaptonemal complex (Sycp3) in mice replicates many of the chromosomal segregation deficiencies known to occur during meiosis in human oocytes. The ongoing studies of the synaptonemal complex will provide us with a more comprehensive view of the molecular pathways that contribute to aneuploidy in female germ cells and also support the development of more effective human assisted reproductive methods.
|Anna Kuznetsova||Senior lab manager|
|Jian-Guo Liu||Senior lab manager|
|Sonata Valentiniene||Biomedical scientist|
RNF212 is a dosage-sensitive regulator of crossing-over during mammalian meiosis.
Nat. Genet. 2013 Mar;45(3):269-78
Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis.
PLoS Genet. 2012 Feb;8(2):e1002485
Meiosis in mice without a synaptonemal complex.
PLoS ONE 2011 ;6(12):e28255
Bi-orientation of achiasmatic chromosomes in meiosis I oocytes contributes to aneuploidy in mice.
Nat. Genet. 2007 Aug;39(8):966-8
Female germ cell aneuploidy and embryo death in mice lacking the meiosis-specific protein SCP3.
Science 2002 May;296(5570):1115-8