Arne Lindqvist's Group
We are interested in how human cells decide to divide, the signalling regulating mitotic entry and its interplay with DNA-damage checkpoints.
Entry into mitosis is regulated by a complex signalling network, converging on activation of mitotic Cyclin-Cdk complexes. To prevent transmission of mutations, this network is strictly controlled by DNA-damage checkpoints. In fact, DNA-damage checkpoint signalling and mitotic entry signalling can both modify each other. Because of these modifications, the decision to enter mitosis is taken differently after recovery from a DNA-damage checkpoint compared to during unperturbed growth.
We focus on two main approaches to study the mitotic entry network and its interplay with DNA-damage checkpoints. First, we investigate the function and regulation of individual proteins involved in these processes. Second, we assemble experimentally derived biophysical models to study how these proteins function together. To support both of these approaches we also focus on developing novel microscopy-based techniques.
|Karen Akopyan||Senior lab manager|
|Arne Lindqvist||Senior researcher|
|Anna Middleton||R&D trainee, Graduate Student|
Assessing kinetics from fixed cells reveals activation of the mitotic entry network at the S/G2 transition.
Mol. Cell 2014 Mar;53(5):843-53
Monitoring kinase and phosphatase activities through the cell cycle by ratiometric FRET.
J Vis Exp 2012 Jan;(59):e3410
Boosting and suppressing mitotic phosphorylation.
Trends Biochem. Sci. 2011 Nov;36(11):578-84
Wip1 confers G2 checkpoint recovery competence by counteracting p53-dependent transcriptional repression.
EMBO J. 2009 Oct;28(20):3196-206
Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery.
Nature 2008 Sep;455(7209):119-23