Kirsty Spalding's Group

Upper row from left: Christina Jones Endre Kiss Maria Kutschke Carolina Hagberg Lena Appelsved Debajit Bhowmick Firoozeh Salehzadeh Qian Li. Bottom row from left: Keng-Yeh Fu Beatriz Rosón Burgo Kirsty Spalding Helena Silva Cascales Anitta Kinga Sárvári Andrea Mosqueda.

Our lab is primarily interested in investigating the origin and turnover of adipocytes, their progenitor cells and lipid stores in lean and obese individuals.

Obesity is increasing in an epidemic manner in most countries and constitutes a public health problem by enhancing the risk for diseases such as diabetes, fatty liver disease and atherosclerosis. Together these diseases form a cluster referred to as the metabolic syndrome.

An important factor behind obesity complications is the fat cell (adipocyte). Adipocytes release large amounts of free fatty acids which regulate insulin action and the metabolism of glucose and lipids in skeletal muscle and liver. They also secrete hormones, inflammatory proteins and other substances with peripheral effects on blood vessels, appetite, energy homeostasis, blood pressure and glucose as well as lipid metabolism. Thus, disturbances in the normal functioning of fat cells have significant consequences on the health of an individual. Despite the importance of the fat mass very little is known about the maintenance of fat cells in humans, how different fat depots are regulated and how, or if, this is altered in obesity. 

Lipid turnover and cell age are studied using radiocarbon dating. By studying cell turnover in a variety of different adipose depots (such as various subcutaneous adipose depots as well as visceral depots) we aim to better understand the regulation of the fat mass in humans. Understanding the dynamics of adipocyte turnover may shed new light on potential treatments for obesity.

Group Members

Kirsty Spalding, Senior researcher

Originally from Australia, I completed my PhD in the field of neuroscience at the University of Western Australia. This was followed by postdoctoral studies at the Karolinska Institute, Sweden, where I switched from studying neuronal cell death to neuronal birth.  During the later stages of my postdoctoral period I started a side project looking at fat cell turnover in human adipose tissue. This project developed in to several more projects and now the major interest of my research group is the turnover and maintenance of human adipose tissue, in health and pathology.

   

Lena Appelsved, Laboratory engineer

I have a Bachelor of Molecular Biology and Biotechnology at KI. I have been working in the Spalding group as a lab manager since 2012. Between 1993 and 2012 I was working as a research scientist at AstraZeneca R&D, CNS and Pain. I have 3 years experience at a Clinical Immunology lab at Huddinge hospital as a laboratory technician. I have many administrative tasks in the group, but also contribute to various scientific project. 

   

Endre Kiss, flow cytometrist/research technician

My main tasks in the laboratory include the operation of a Beckman Coulter MoFlo XDP high-speed cell sorter, performing flow citometric analysis/sorting of primary human cells and processing samples for cell turnover projects. I am also responsible for the general maintenance of a Zeiss LSM700 laser-scanning confocal microscope.

   

Carolina Hagberg, Postdoc

I am a senior postdoctoral scientist studying novel aspects of human adipocyte biology in relation to obesity and diabetes. I mainly work with human clinical tissue samples to interrogate the correlation between patient metabolic health and white adipose tissue homeostasis, including how the expanding fat mass responds to dysregulated trophic signaling pathways in diabetes. I have also been developing novel methods to study adipocytes, including adipocyte flow cytometry and staining techniques.

   

Firoozeh Salehzadeh, Postdoc

I am studying the heterogeneity of human adipocytes, using single cell technologies to understand the cellular complexities and functional heterogeneity of adipocytes in different human fat depots. 

   

Christina Jones, Postdoc

I am investigating podocyte regeneration in the human kidney, using radiocarbon dating of human cells to define cell turnover dynamics in kidney health and disease.

   

Qian Li, PhD student

I am studying the biological mechanisms underlying adipocyte turnover and obesity development, performing in vivo and in vitro studies with mouse and human white adipose tissues.

   

Keng-Yeh Fu, PhD student

I am investigating human mature adipocyte and preadipocyte turnover rates from both visceral and subcutaneous fat depots using radiocarbon dating, in lean and obese individuals.

   
Debajit Bhowmick, Flow Cytometrist

I am responsible for the design, execution & data interpretation of flow cytometric experiments. We have our own 4 laser 9 color MoFlo XDP in lab which we use extensively for high speed sorting (bulk & single cell). I help my colleagues to run the CyToflex in our department. We also use Canto II for ploidy analysis.

   

Maria Kutschke, Laboratory technician

I support most projects in the group. My main tasks include human adipocyte isolation, mouse studies and cell/tissue preparation for FACS, microscopy, stereology and a range of other analytical techniques. I also have extensive experience in mitochondrial bioenergetics.

   

Beatriz Rosón Burgo, Postdoc, Computational biologist

I am applying bioinformatics analysis on single-cell RNA sequencing data to study the heterogeneity of human adipocytes.

   

Helena Silva Cascales, Post doc

I am studying the molecular mechanisms mediating adipocyte turnover in white adipose tissue from healthy and metabolically unhealthy patients to understand the factors that contribute to the development of metabolic disease.

   

Anitta Sárvári, Post doc

I am studying the molecular background of metabolically impaired obese compared to healthy adipose tissue to determine the factors that influence white adipocyte viability and turnover.

   

Selected Publications

Dynamics of human adipose lipid turnover in health and metabolic disease.
Arner P, Bernard S, Salehpour M, Possnert G, Liebl J, Steier P, et al
Nature 2011 Sep;478(7367):110-3

Impact of fat mass and distribution on lipid turnover in human adipose tissue.
Spalding K, Bernard S, Näslund E, Salehpour M, Possnert G, Appelsved L, et al
Nat Commun 2017 May;8():15253

Transplanted Bone Marrow-Derived Cells Contribute to Human Adipogenesis.
Rydén M, Uzunel M, Hård J, Borgström E, Mold J, Arner E, et al
Cell Metab. 2015 Sep;22(3):408-17

Dynamics of fat cell turnover in humans.
Spalding K, Arner E, Westermark P, Bernard S, Buchholz B, Bergmann O, et al
Nature 2008 Jun;453(7196):783-7

Forensics: age written in teeth by nuclear tests.
Spalding K, Buchholz B, Bergman L, Druid H, Frisén J
Nature 2005 Sep;437(7057):333-4

Retrospective birth dating of cells in humans.
Spalding K, Bhardwaj R, Buchholz B, Druid H, Frisén J
Cell 2005 Jul;122(1):133-43

 

 

Adult Neurogenesis in Humans.
Bergmann O, Spalding K, Frisén J
Cold Spring Harb Perspect Biol 2015 Jul;7(7):a018994

Adrenergically stimulated blood flow in brown adipose tissue is not dependent on thermogenesis.
Abreu-Vieira G, Hagberg C, Spalding K, Cannon B, Nedergaard J
Am. J. Physiol. Endocrinol. Metab. 2015 May;308(9):E822-9

Maintenance of white adipose tissue in man.
Hyvönen M, Spalding K
Int. J. Biochem. Cell Biol. 2014 Nov;56():123-32

Analysis of radiocarbon, stable isotopes and DNA in teeth to facilitate identification of unknown decedents.
Alkass K, Saitoh H, Buchholz B, Bernard S, Holmlund G, Senn D, et al
PLoS ONE 2013 ;8(7):e69597

Dynamics of hippocampal neurogenesis in adult humans.
Spalding K, Bergmann O, Alkass K, Bernard S, Salehpour M, Huttner H, et al
Cell 2013 Jun;153(6):1219-1227

Personal identification of cold case remains through combined contribution from anthropological, mtDNA, and bomb-pulse dating analyses.
Speller C, Spalding K, Buchholz B, Hildebrand D, Moore J, Mathewes R, et al
J. Forensic Sci. 2012 Sep;57(5):1354-60

Developmental BiologyObesityStem Cell Biology