Shupliakov Laboratory

Professor

Oleg Shupliakov

Phone: +46-(0)8-524 839 41
Organizational unit: Department of Neuroscience (Neuro), C4
E-mail: Oleg.Shupliakov@ki.se

Research focus

Membrane vesicles filled with different cargos serve for communication between cells, intracellular compartments and organelles.

We study the molecular and biophysical mechanisms underlying vesicle trafficking in neurons and in particular in their axons and presynaptic nerve terminals.

Our current interest is in the molecular mechanisms, which link the synaptic vesicle cycle with autophagosome-lysosomal and protein degradation pathways. It is known, that effector synaptic proteins and scaffolding molecules, which are involved in coordination and targeting of the effector proteins and membrane lipids, control vesicle trafficking events in the nerve terminal.

Our recent studies show that deletion of certain genes and genetic perturbations of transcription factors implicated in neurodegenerative diseases cause dramatic changes in vesicle trafficking, activation of autophagasome-lysosomal pathway in synaptic terminals and formation of pathological protein aggregates in neurons.

We are aiming at characterizing the signaling mechanisms that regulate the vesicle trafficking events and degradation pathways at synapses and clarify how do they become affected at early stages of neurodegenerative diseases in particular in Parkinson´s disease.

To address our goals we use several model systems such as giant reticular spinal axon in lamprey, Drosophila neuromuscular junction and mammalian neurons, in combination with molecular biology, genetics, cellular imaging techniques, and intracellular recordings.

We believe that our studies will pave the way for identification of therapeutic targets for treatments of neurodegenerative disorders. 

Recent Publication:

O Shupliakov laboratory: Actin provides sufficient Plasma Membrane (PM) tension to mediate Ω-profile shrinking From Wen et al., 2016, Nat Commun.

Selected publications

Actin dynamics provides membrane tension to merge fusing vesicles into the plasma membrane.
Wen P, Grenklo S, Arpino G, Tan X, Liao H, Heureaux J, et al
Nat Commun 2016 Aug;7():12604

Sphingosine 1-phosphate lyase ablation disrupts presynaptic architecture and function via an ubiquitin- proteasome mediated mechanism.
Mitroi D, Deutschmann A, Raucamp M, Karunakaran I, Glebov K, Hans M, et al
Sci Rep 2016 Nov;6():37064

An Endocytic Scaffolding Protein together with Synapsin Regulates Synaptic Vesicle Clustering in the Drosophila Neuromuscular Junction.
Winther , Vorontsova O, Rees K, Näreoja T, Sopova E, Jiao W, et al
J. Neurosci. 2015 Nov;35(44):14756-70

Membrane Charge Directs the Outcome of F-BAR Domain Lipid Binding and Autoregulation.
Kelley C, Messelaar E, Eskin T, Wang S, Song K, Vishnia K, et al
Cell Rep 2015 Dec;13(11):2597-2609

Dopaminergic control of autophagic-lysosomal function implicates Lmx1b in Parkinson's disease.
Laguna A, Schintu N, Nobre A, Alvarsson A, Volakakis N, Jacobsen J, et al
Nat. Neurosci. 2015 Jun;18(6):826-35

The dynamin-binding domains of Dap160/intersectin affect bulk membrane retrieval in synapses.
Winther , Jiao W, Vorontsova O, Rees K, Koh T, Sopova E, et al
J. Cell. Sci. 2013 Feb;126(Pt 4):1021-31

Group members

Gianvito Arpino - PhD student

Albin Blomquist - MS Student

Oleg Shupliakov - Professor

Elena Sopova - Research assistant

Olga Vorontsova - Research engineer

Neurosciences