Petzold Group

Assistant Professor

Katja Petzold

Phone: +46-(0)8-524 876 47
Organizational unit: Katja Petzold Group
E-mail: katja.petzold@ki.se

Mobile: +46 070 588 17 96

Visiting address:

Room A2-424
Scheeles väg 2
Stockholm, 171 77
Sweden

Please visit petzoldlab.com for more information.

Research

Our research group is interested in understanding how RNAs change their structures in order to perform function. Until recently, only snapshots of molecules could be observed, hiding their mode of operation. We employ Nuclear Magnetic Resonance (NMR), and other biophysical techniques, to investigate the molecular mechanism of RNA function. When function of these molecular machines becomes apparent, it also provides a variety of unique new drug targets. The lab develops methods in NMR and RNA biochemistry to address these questions. Current projects include viral, bacterial and eukaryotic regulatory RNAs, e.g. microRNAs, ribosomal RNAs or RNA from HBV.

How RNA move – excited states of RNA (Nature 2012):

A-site

DIS

Transactvaton Response Element Ribosomal A-site Dimerization Initiation Site

News

2014  
January the story of Petzold lab begins
July first RNA is produced in the wet-lab
August Postdoctorial Fellow Carolina Fontana joins the lab
September Bruker delivers our new 600MHz NMR machine -work can begin
October PhD student candidate Lorenzo Baronti joins the lab
October Katja recieves the Ragnar Söderberg Fellowship in Medicine http://ragnarsoderbergsstiftelse.se/
November Master student Oana Florean joins the lab
2015  
January Lorenzo gets admitted to PhD studies at KI
January PhD student candidate Hampus Karlsson and Master student Mona Farshchian are joining the lab
January Nature article describing GU and GT mismatches was accepted
March Postdoctoral Fellow Emilie Steiner is joining the lab
March Our Nature paper is finally out, with a commentary on the impact on DNA
April Postdoctoral Fellow Ileana Guzzetti is joining the lab. She will be working jointly with the E. Andersson Lab (KI-CMB)
April Katja receives the Ingvar Carlsson Award

07/2015

The Ingvar Carlsson award ceremony gets covered in Kemivärlden Biotech.

09/2015

A new paper related to the petzold lab is published: Modulating RNA Alignment Using Directional Dynamic Kinks: Application in Determining an Atomic-Resolution Ensemble for a Hairpin using NMR Residual Dipolar Couplings.

10/2015

Carolina Fontana received a Assistant Professor position at the Department of Chemistry, University of the Republic, Uruguay. Congratulations and good luck with your future research Carolina!

10/2015

This years MBB Keynote lecture presents work in RNA and DNA modifications and G-quadruplex formation.

11/2015

Luca Retattino joins the lab as a project student!

11/2015

The Swedish Cancer Society has funded our work on miR-34a.

2016  

02/2016

The Petzold Lab co-organizes the workshop in Stockholm June 12-15, where experts in RNA biology and RNA structure meet

04/2016

Postdoc Judith Schlagnitweit and Master student Karen Schriever joins the group.

04/2016

During 2016, Bachelor student Noah Hopkins joins the Petzold lab for a project.

05/2016

Lorenzo Baronti received the travel grant from the Biophysical Society for the “CHIANTI WORKSHOP ON MAGNETIC RESONANCE FOR CELLULAR STRUCTURAL BIOLOGY” for his work on miRNA targeting.

08/2016

Congratulations Luís Silva on receiving an Erasmus scholarship for his Master Thesis!

08/2016

Master student Luís Silva will join the Petzold lab for a one year project.

08/2016

Master student Karen Schriever finishes her project in the Petzold lab.

08/2016

Our paper “Visualizing Transient Watson-Crick Like Mispairs in DNA and RNA Duplexes” Nature, 2015, is as of March/April 2016 a highly cited paper  (received enough citations to place it in the top 1% of its academic field) based on a Web of Knowledge/ISI.

10/2016

Congratulations to Emilie (first author), Judith and our collaborator Patrik Lundström for their great work to extend R1ρ timescales and probes with 1H R1ρ.

10/2016

Katja and Emma receive the Knut och Alice Wallenberg Project grant. Our project on miRNA targeting got funded by the prestigious Wallenberg foundation – Thank you!!

10/2016

Katja received the Sven och Ebba-Christina Hagbergs Pris for her work on RNA structure and function using NMR. This price is given to young group leaders who have shown potential.

Selected Publications

Visualizing transient Watson-Crick-like mispairs in DNA and RNA duplexes.
Kimsey I, Petzold K, Sathyamoorthy B, Stein Z, Al-Hashimi H
Nature 2015 Mar;519(7543):315-20

Visualizing transient low-populated structures of RNA.
Dethoff E, Petzold K, Chugh J, Casiano-Negroni A, Al-Hashimi H
Nature 2012 Nov;491(7426):724-8

Synthesis, screening and computational investigation of pentacycloundecane-peptoids as potent CSA-HIV PR inhibitors.
Makatini M, Petzold K, Arvidsson P, Honarparvar B, Govender T, Maguire G, et al
Eur J Med Chem 2012 Nov;57():459-67

Semiconstant-time P,H-COSY NMR: analysis of complex mixtures of phospholipids originating from Helicobacter pylori.
Petzold K, Olofsson A, Arnqvist A, Gröbner G, Schleucher J
J. Am. Chem. Soc. 2009 Oct;131(40):14150-1

EASY ROESY: reliable cross-peak integration in adiabatic symmetrized ROESY.
Thiele C, Petzold K, Schleucher J
Chemistry 2009 ;15(3):585-8

Conserved nucleotides in an RNA essential for hepatitis B virus replication show distinct mobility patterns.
Petzold K, Duchardt E, Flodell S, Larsson G, Kidd-Ljunggren K, Wijmenga S, et al
Nucleic Acids Res. 2007 ;35(20):6854-61

Available Positions

Applications for Master Students are welcome in the field of NMR or RNA biochemistry. Students have preferably a background in biochemistry, chemistry, molecular biology or physics with an interest in inter-disciplinary research.

We are always looking for talented Master, PhD students and Postdocs. Please send a 1 page coverletter, 2 page CV, publication record and list of references to katja.petzold'at'ki.se.

Research Group

Katja Petzold Assistant Professor
Emilie Steiner Postdoctoral Fellow
Lorenzo Baronti PhD student
Hampus Karlsson PhD student
Ileana Guzzetti Postdoctoral Fellow joined with the Andersson lab
Judith Schlagnitweit Postdoctoral Fellow
Luca Retattino PhD student
Luis Silva Master student
Noah Hopkins Project student 

Alumni

Oana Florean

Mona Farshchian

Carolina Fontana

Karen Schiever

Links