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Stem cells, engineering and FACS

Cutting edge fluorescence-activated cell sorting (FACS) technologies are mandatory elements in the toolbox of a leading medical university. Karolinska Institutet also provide state of the art iPS technology, and platforms for material engineering.

FACS Biomedicum

The Biomedicum flow cytometry core facility (BFC) is a shared resource laboratory that will provide researchers with the capability of performing a wide range of flow cytometry experiments.

Biomedicum Flow Cytometry Core Facility
 

High Throughput Genome Engineering

The High Throughput Genome Engineering (HTGE) facility provides affordable access to high throughput functional genomic screens using the CRISPR/Cas9 system in cell lines. Pooled CRISPR/Cas9 screening enables parallel interrogation of thousands to tens of thousands of genes for involvement in biological processes of interest. HTGE provides access to verified lentiviral CRISPR guide libraries for whole genome and more targeted loss- and gain of function studies (CRISPR knock-out, CRISPR inhibition, CRISPR activation). They also offer generation of stable Cas9-expressing lines in users’ cells of interest.

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iPS Core Facility

The reprogramming facility provides a reprogramming service to supply scientists with human induced pluripotent stem (iPS) cells. Patient-specific iPS cell lines opens up unique opportunities to in vitro study and create cellular models of human disease. The facility reprograms human fibroblast cells using Sendai viruses, which is a non-integrating method.

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Karolinska Genome Engineering

The Karolinska Genome Engineering Facility (KGE) are specialists in CRISPR/Cas technology, and it is their mission to facilitate and accelerate your research by providing flexible and affordable gene-editing services in cells. Your cell type, your edit – from strategy design to delivery of a cell pool with quantified allele frequency. KGE is a branch of the High Throughput Genome Engineering, a national SciLifeLab facility (see above).

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MedH Flow Cytometry Facility

The Flow Cytometry Facility at the Department of Medicine, Huddinge (South Campus) offers a broad range of high quality services including assistance with experimental design, training for independent users, and operator-assisted services for cell analysis and cell sorting.

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Micro and Nanofabrication Core Facility

The Micro and Nanofabrication Core Facility (MCF) offers a diverse range of Micro and Nanostructuring techniques for a wide scope of applications in the fields of life-science and medicine. The MCF aims to bridge the gap between engineering and biology at Karolinska Institutet and to provide common grounds with respect to suitable material of use of modular design and tunable structures for biomedical purposes.

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Murine Stem Cells Unit

Murine Stem Cells Unit (MSCU) provides services in the field of mouse embryonic stem cells and related techniques including characterization, differentiation, and cryopreservation of genetically modified mouse embryonic stem cell (mESC) lines. MSCU is a well-resourced laboratory with all the equipment necessary to conduct experiment in pre-implantation embryology and stem cells.

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Stem Cell & Organoid Core Facility

The Stem Cell & Organoid Core Facility (CMM – OrganoidCellStem) facility is located at the Centre for Molecular Medicine and has the aim to facilitate the generation and distribution of human organoids from pluripotent stem cells, and to develop in vitro cellular models for academy and industry. The facility also provides support for the maintenance and differentiation of established human pluripotent stem cell lines – both human embryonic stem cells and human induced pluripotent stem cells – and training for researchers.

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VirusTech Core Facility

Genetically engineered viruses provide powerful tools for introducing desired genes in cells. To facilitate the use of this state-of-art methodology, the VirusTech core facility at KI's Department of Cell and Molecular Biology specializes in the production, purification and concentration of such virus particles.

The VirusTech Core Facility