Omics and high-throughput
On this page we have collected core facilities and resources within proteomics and genomics and related techniques located at our two main campuses. Several of the listed core facilities are also a part of the national research facility SciLifeLab.
Bioinformatics and Expression Analysis
Chemical Proteomics and Proteogenomics
Chemical Proteomics & Proteogenomics (CPP) is a national facility that offers state-of-the art mass spectrometry-based proteomics support to the Swedish research community. The facility also offers expert support in experimental planning, MS-analysis and data analysis related to proteogenomics and chemical proteomics. The activities are shared between two sites in Stockholm: one at SciLifeLab and one at Karolinska Institutet. The chemical proteomics and proteogenomics facility is also a node in Swedish National Mass Spectrometry Facility, BioMS.
Chemical Proteomics Core Facility
The facility is located at Biomedicum, Campus Solna, and specializes in supporting target identification and validation, keys and bottlenecks of drug discovery and development. It is also connected to the Chemical Proteomics and Proteogenomics node at SciLifeLab.
Clinical Proteomics Mass Spectrometry
Clinical Proteomics Mass Spectrometry (MS) is a core facility that offers an opportunity to analyze the proteome composition of biological samples. The facility provides expertise and services in a wide range of MS-based proteomics methods with focus on in-depth quantitative proteomics and clinical proteomics. The facility also offers expert support in experimental design as well as downstream data analysis to ensure a high quality service.
The team behind this facility continuously strive to develop and implement cutting edge research methods in their services. This is exemplified by the level of data produced by the HiRIEF method used for in-depth proteomics, and by our continuous development of bioinformatics methods for optimal use of the generated data. The financial support for the facility is provided by the Stockholm county council (SLL).
Eukaryotic Single Cell Genomics
The Eukaryotic Single Cell Genomics facility aims at providing single cell transcriptomics and genomics services to the scientific community. Single cell analysis is an emerging technology that allows for the exploration of the transcriptome and genome content of individual cells. The facility offers streamlined single-cell RNA sequencing as well as whole genome DNA amplification to the scientific community in Sweden and beyond. The facility is of national interest and part of the SciLifeLab.
Genomic Medicine Center Karolinska
Genomic Medicine Center Karolinska (GMCK) is a KI/SLL core facility for translational research, clinical trials and diagnostics, focusing on transitioning of next generation sequencing (NGS) from basic science to clinical diagnostic settings. The facility supports KI and KS researchers and clinical users in aspects covering experimental design, data generation, data analysis and solutions for clinical decision support. The facility is operates as a joint unit between the Clinical Genomics facility at SciLifeLab and a genomics unit at Karolinska university laboratory When applicable, the facility will support researches by facilitating the contact with other genomics and high-throughput facilities at KS(/SLL), KI and Scilifelab.
High Throughput Genome Engineering (HTGE)
HTGE is a national, ScilIfelab funded facility based in KI’s new research building, Biomedicum. The facility provides affordable access to pooled genome-wide loss- and gain-of-function screens using the CRISPR/Cas9 system in cell lines. A second branch, Karolinska Genome Engineering (KGE) is currently starting up as a new Karolinska core facility. KGE provides custom gene-editing in cell lines (knock-outs, knock-ins, point mutations, etc.). In collaboration with the Karolinska Center for Transgene Technologies (KCTT), KGE can also help with designing CRISPR/Cas9 strategies for the creation of mouse models.
KI Gene – Genetic Analysis
KI Gene offers Sanger DNA sequencing with fast turn-around time, gene expression analysis and analysis of genetic variations including SNPs, microsatellite markers and insertion/deletion polymorphisms.
Laboratories for Chemical Biology
Laboratories for Chemical Biology (LCBKI) is a KI core facility and a part of the gouvernment-funded Chemical Biology Consortium Sweden (CBCS) and SciLifeLab. The facility comprises compound handling labs, hosting the SciLifeLab compound collection of about 200 000 molecules. It also offers screening labs such as microtiter format liquid handling robotics and a wide range of plate readers, as well as chemistry labs with equipment and instrumentation for organic chemistry synthesis and analysis.
Mutation Analysis Facility
The purpose and mission of the Mutation Analysis core Facility (MAF) is to help scientists at Karolinska Institutet, its associated hospitals, and other academic institutions to perform genetic, epigenetic and genetic-epidemiological research in order to identify and assess genetic factors involved in different disease processes. The facility offers techniques for targeted DNA methylation, genome-wide DNA methylation, genome-wide genotyping, targeted SNP genotyping as well as genome-wide expression analysis.
National Mass Cytometry Facility
This facility provides state-of-the art Mass Cytometry analysis to the entire Swedish research community. The facility belongs to the Affinity proteomics platform within SciLifeLab and consists of two nodes, one in Stockholm and one in Linköping. Together they perform standardized Mass Cytometry experiments on customer provided samples using validated panels of metal-coupled antibodies. They also perform technology development of new experimental assays and reagents as well as novel data analysis tools.
Protein Science Facility
The Protein Science Facility (PSF) provide users with protein production services and access to the state-of-the-art infrastructure for Macromolecular X-ray crystallography (MX) and biophysical protein characterization available at the Department of Medical Biochemistry and Biophysics (MBB). PSF is a National Facility for protein production within SciLifeLab and SWEDSTRUCT.
The Proteomics Biomedicum facility provides services using best available proteomics techniques and methods with focus on label-free quantitative proteomics.
More about Proteomics Biomedicum