Confocal Microscopes at BIC

The BIC facility has several confocal microscopes with various configurations.

Zeiss LSM980-Airy2

The LSM980-Airy2 confocal is built around an inverted microscope with a motorized x/y-stage and filter-less control of fluorescence emission. The system is equipped with an Airy detector2 for super resolution capacity. Furthermore, the LSM980 microscope has a temperature control module for live cell imaging.

Specifications:
  • Laser lines (nm): 405, 445,488, 561, 594, 639
  • Objectives: 5X, 10X and 20X air/dry, 40X water and 63X oil.
  • Extra: Super resolution, Lambda imaging, Confocal reflection microscopy. Motorized x/y-stage allows tiling, live cell imaging
  • Software: For PC: ZEN lite, for Mac (and alternative for PC): FiJi.

Zeiss LSM900-Airy2

The Zeiss LSM900-Airy2 confocal is built around an inverted microscope with a motorized x/y-stage and an Airy detector2 for super resolution capacity. 

Specifications:
  • Laser lines (nm): 405, 488, 561, 640.
  • Objectives: 5X, 10X and 20X air/dry, 40X water, 63X oil.
  • Extra: Super resolution, Motorized x/y-stage allows tiling, Stitching module for best adjustments of the tiles
  • Software: For PC: Zen lite. For MAC (and alternative for PC): ImageJ/Fiji.

Zeiss LSM880

The LSM880 confocal is built around an inverted microscope with a motorized x/y-stage and filter-less control of fluorescence emission.

Specifications:

  • Laser lines (nm): 405, 458, 488, 514, 561, 633.
  • Objectives: 5X, 10X and 20X air/dry, 40X oil and 63X oil.
  • Extra: Lambda imaging, Confocal reflection microscopy. Motorized x/y-stage allows tiling.
  • Software: For PC: ZEN lite, for Mac (and alternative for PC): FiJi.

Zeiss LSM800-Airy

The Zeiss LSM800-Airy confocal is built around an inverted microscope with a motorized x/y-stage and and an Airy detector for super resolution capacity. Fixed filters and/or filterless emission window control.

Specifications:

  • Laser lines (nm): 405, 488, 561, 640.
  • Objectives: 5X, 10X and 20X air/dry, 63X water, 63X oil.
  • Extra: Super resolution, Lambda imaging, DIC with 63X water objective, Motorized x/y-stage allows tiling, Stitching module for best adjustments of the tiles.
  • Software: For PC: Zen lite. For MAC (and alternative for PC): ImageJ/Fiji.

Zeiss LSM800

The Zeiss LSM800 confocal is built around an inverted microscope with motorized x/y-stage, DIC capacity and stitching module. Fixed filters and/or filterless emission window control.

Specifications:

  • Laser lines (nm): 405, 488, 561, 640.
  • Objectives: 5X, 10X and 20X air/dry, 40X water, 100X oil.
  • Extra:  Lambda imaging, DIC with 20X, 40X and 100X objectives, Motorized x/y-stage allows tiling, Stitching module for best adjustments of the tiles.
  • Software: For PC: Zen lite. For MAC (and alternative for PC): ImageJ/Fiji.

Zeiss LSM710

The LSM710 confocal is built around an inverted microscope with motorized x/y-stage and filter-less control of fluorescence emission. It also has a stage top incubator for live cell imaging.

Specifications:

  • Laser lines (nm): 405, 458, 488, 514, 561, 633.
  • Objectives: 10X and 20X air/dry, 25X imm (oil/glyc/water), 40X water, 63X oil.
  • Extra:  Lambda imaging. Confocal reflection microscopy, DIC with 20X, 25X, 40X W and 63X oil objectives, Motorized x/y-stage allows tiling, Stage top incubator for live cell imaging.
  • Software: For PC: Zen lite. For MAC (and alternative for PC): ImageJ/Fiji.

Zeiss LSM700

The LSM700 confocal is built around an inverted microscope equipped with motorized x/y-stage and fixed emission filters. It has a variable secondary dichroic (VSD) to allow fine tuning of the emission windows.

Specifications:

  • Laser lines (nm): 405, 488, 555, 639.
  • Objectives: 5X, 10X, and 20X air/dry, 40X water, 63X oil.
  • Extra: Lambda imaging, DIC with 10X, 20X, 40X water and 63X oil objectives, Motorized x/y-stage allows tiling.
  • Software: For PC: Zen lite. For MAC (and alternative for PC): ImageJ/Fiji.

Olympus FV1000

The Olympus FV1000 confocal is built around an inverted microscope with a manual x/y-stage.

Specifications:

  • Laser lines (nm): 405, 473, 559, 635.
  • Objectives: 4X, 10X, 20X and 40X air/dry, 60X water and 60X oil.
  • Extra: DIC, High Dynamic range imaging, Reduced user fee by 50%.
  • Software: :For PC: Olympus FV10-ASW, for MAC (and alternative for PC): ImageJ/Fiji.

Leica SP5

The Leica SP5 confocal is built around an inverted microscope equipped with microscope incubator for live cell imaging, tunable laser to pinpoint excitation maximum. Limited to microscope slide or 60mm Petri dish formats.

Specifications:

  • Laser lines (nm): 405, tunable white laser (WLL) in range 470-670, Argon laser (458, 476, 488, 496, 514), 561, 633.
  • Objectives: 10X and 20X air/dry, 63X and 100X oil.
  • Extra: Optional resonance scanner for extra fast scanning, Microscope incubator for live cell imaging, Reduced user fee by 50%.
  • Software: For PC: Leica Las AF or LAS X. For Mac: ImageJ/Fiji.

If you want more details about the different microscopes we have compiled a sheet for you under More information. The information may be useful when choosing between different systems. 

You are always welcome to contact us if you need further information or guidance.

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More Information