High Content Automated Systems at BIC
BIC facility is equipped with a diverse collection of automated, high content imaging systems for imaging tissue sections on slides, multiwell plates (6-384 wells), and multi-day live imaging experiments.
Sartorius IncuCyte S3
The IncuCyte S3 system is a dedicated imaging system inside a cell culture incubator with perfect temperature (37° C), 5% CO2, and humidity control. The system allows long-term transmitted light and GFP/RFP fluorescence imaging of adherent, non-adherent cells, and spheroids/organoids in diverse multi-well plate formats, ranging from 6 to 384 wells, and ultra-low attachment (ULA) U- and V-bottom plates. The system can be used by up to three different users with a maximum of 6 plates simultaneously.
Specifications
- Stage inserts for: slides/multiwell plates (incl. 96 and 384well)
- Light Source: LEDs
- Fluorescent channels: GFP, RFP
- Transmitted channels: brightfield
- Objectives: 5x, 10x, 20x (all air/dry)
- Acquisition & Analysis Software: IncuCyte-2019BRev2
- Special Features:
- located inside the cell culture incubator
- 3 trays, 6 multiwell plate positions
- multi-day imaging sessions for up to 3 users simultaneously
Zeiss Axio Scan.Z1 Slide Scanner
The AxioScan.Z1 is an automated slide scanner which can process up to 100 slides in a single imaging session. This instrument is capable of fluorescence imaging as well as brightfield imaging with colorimetric detection (e.g. HE/DAB) using air objectives. The system can automatically detect the location of samples on slides, create focus maps, followed by scanning the images.
Specifications
- Stage inserts for: slides 76mm x 24-26mm
- Light Source: VIS-LED for brightfield. Colibri 7 LED for fluorescence (385, 430, 475, 567, 630 & 735nm)
- Fluorescent channels: DAPI, CFP, FITC, YFP, TRITC, mCherry, Cy5, Cy7
- Transmitted channels: brightfield
- Objectives: 5x, 10x, 20x, 40x (all air/dry)
- Camera fluo: Hamamatsu Orca Flash 4.0 V3 (16-bit, 2048x2048, 6.5 mm pixel size)
- Camera color: Hitachi HV-F203 (14.bit, 1600x1200)
- Acquisition Software: Zen Blue v3.1
- Special Features:
- Up to 100 slides in one experiment
- Fluorescence and colorimetric imaging