IncuCyte live cell analysis Core facility in BioClinicum
OnkPat/Theme Cancer Core facility is available for staff at the Department of Oncology-Pathology in addition to research Theme Cancer in BioClinicum. New users need an introduction to get authorized. Contact information to responsible persons can be found below. Before using this core facility send your certificate from KI laboratory safety introduction to Paula Mannström. Access to the building is also required.
IncuCyte SX5 SARTORIUS
Room nr: U230 05 2510
Responsible persons: Paula Mannström Katja Pokrovskaja
Booking calendar: The booking and charging are done in the iLab booking system
Instrument
The instrument is located in a shared cell lab, in the lower left incubator with the Control unit placed on top of the upper left incubator. The plates at positions 1-5 can be used for maximum 1 week. Plate at position 6 can be used for minimum 1 hour and maximum for 3 hours. Plates waiting for the scan or removed from the IncuCyte after the end of the experiment should be temporarily stored in the lower right incubator. The available software modules are:
- Standard
- Image lock
- Scratch Wound
- Dilution cloning
- Chemotaxis
- Spheroid
- AI scan
New users
This equipment is a core facility for Research Theme Cancer at BioClinicum and is accessible for authorized persons only. All users should have passed KI´s Laboratory safety introduction. Send certificate to Paula Mannström
The new users should contact Katja Pokrovskaja Tamm to book and receive an introduction to the routines for this facility, a brief training for operating IncuCyte and some instruction material to become an authorized user. Get started with iLab booking system before the introduction. Each user will also become a member of the “GRP_IncuCyte users” Outlook group. The IncuCyte software can be downloaded for free, either from the memory stick or Sartorius home page, see instructions below.
General Work Rules
The defined rules and work procedures for using IncuCyte must be strictly followed:
- the instrument is located in a BSL2 cell laboratory. It is mandatory to use the lab coat and gloves and follow the rules for this type of laboratory.
- Restrict usage to two plates per time and per person. To book more than one plate or for more than 1 week you must send a notification to the “GRP_IncuCyte users” group to get a “green light”.
- Insert your plate(s) into the tray outside of IncuCytem and gently place the tray in its position.
- Bring your own gloves and materials. Do not use anything from the group in the shared lab besides ethanol/disinfection located in the fridge.
- Clean up after yourself. Remove your plate and discard it and all the materials in your own lab.
- Archive and transfer your raw data files after finished experiments either to your own external hard drive or to your personal network drive.
- For data analysis, IncuCyte software can be downloaded for free, see the link below (for PC only). There are also two data analysis computers with installed software in J6:14 and J6:10 with booking through ClusterMarket; to get an account, contact Paula Mannström.
- Delete archived files regularly from the computer! The full hard disk slows down all the operations!