Bacteria lab Core facility at BioClinicum
OnkPat/Theme Cancer Core facility is available for staff at the Department of Oncology-Pathology in addition to research Theme Cancer in BioClinicum. New users need an introduction to get authorized. Contact information to responsible persons can be found below. Before using this core facility send your certificate from KI laboratory safety introduction to Paula Mannström. Access to the building is also required.
Room nr: U230 06 4200
Responsible persons: Kristina Ihrmark Lundberg, Sara Abu Ajamieh, Alkinoos Polychronopoulos
Booking calendar: Find paper calendars at the site for specific equipment.
General information
The room is equipped for bacterial culture, mainly E.coli, for generation of plasmid DNA, recombinant proteins and other similar applications for researchers at BioClinicum. The permit for activities in the room falls under the common GMM F-permit for BioClinicum. All applications performed in the rooms should have been risk assessed prior to initiated work.
Equipment available for use. The room is equipped with the ventilated cabinet for sterile work, bacterial shakers, heating cabinets, table-top centrifuges.
A new Eppendorf centrifuge 5910R GLP S-4XUNIV TUBE/P has been purchased with a fast angle rotor that can be used for high speed (15.000 rpm at 4-degree C) centrifugation in Falcon tubes for plasmid preparations. Only users of the bacteria lab who received an introduction will get a log-in to use the centrifuge for bacteria work and subsequent applications (DNA and protein precipitation, etc.). Fill in the logbook after use (find at the site).
To become a user. New users are requested to contact responsible person for an initial introduction; thereafter contact Theme Cancer coordinator Paula Mannström to be added to the user list and to the Outlook group: GRP_Bacterial lab users.
The users are obliged to keep the room tidy and dispose of all the contaminated trash in the yellow waste container, see guide-lines in the room. The contaminated glass or plastic ware requires de-contamination prior to be left for washing/autoclaving, see special guide-lines in the room. For further information and explanations, if needed, please, contact Kristina Ihrmark Lundberg, Sara Abu Ajamieh or Alkinoos Polychronopoulos (group Per Kogner).