Which microscope does my experiment require?

On this page you can find out what microscopes are required for your experiments.

  • If you want to image fast and have a flat sample (cell monolayer), you probably need a widefield system (Tweety, Orion, Pegasus).
  • Confocals (point scanning or multipoint) are ideal to image samples that are 10-100 um thick (Dragon, Pegasus, Tweety, Victor).
  • For even thicker samples that are transparent or cleared, you will need our light sheet (Aurora).
  • TIRF is used to image the area of the cell immediately in contact with the coverslip (Pegasus, Victor).
  • If the resolution needed is between 100- 200 nm, you will need superresolution SIM (Orion).
  • With our micropatterning system, you can attach proteins at the bottom of a dish, a multiwell plate or on a gel, using the pattern of your choice then study their interaction with cells. You can also make custom-designed microwells (Primo on Tweety).
  • Use our laser-capture microdissection system to cut part of a tissue for RNA analysis or Mass Spectrometry (Minerva).
  • Polarization microscopy is used to quantify collagen in tissue (Dragon).
  • On most of our systems, you can image live samples for hours/days (Aurora, Pegasus, Victor, Tweety)
  • Running a screen is easy on all our Nikon system thanks to the high content screening JOBS module (Dragon, Orion, Pegasus, Tweety, Victor)
  • The image analysis software on our VDI or the Remote Desktop servers will help you extract numbers from multidimensional image files: xy, z, multicolour, time...
GI
Content reviewer:
27-05-2024