Microscopes

  • 1 Zeiss single and multiphoton confocal (Zeus)
  • 4 Nikon confocals (Orion, Tweety, Dragon, Pegasus)
  • 2 Nikon spinning disk confocals (Victor and Tweety)
  • 4 Nikon widefield microscopes (Victor, Orion, Tweety, Minerva)
  • 2 Nikon TIRF microscopes (Orion, Victor)
  • 1 Nikon STORM super resolution microscope (Orion)
  • 1 M-Squared Light Sheet Microscope (Aurora)
  • Nikon JOBS High content screening module (Dragon, Tweety, Orion, Pegasus, Victor, Minerva)
  • 1 Primo (Alveole) micropatterning and micromanufacturing device (Tweety)
  • 1 MMI laser capture microdissection and cell picking system (Minerva)
  • 1 MMI Slide scanner with a colour camera (Minerva)
  • Imaris, NIS-Element, Zen, Fiji, Cell Profiler, Deconvolution, ImSpector, MatLab, Python, CTFire, Omero

Some of our confocals are equipped with fast resonant scanners, and spectral detectors.
Most of our microscopes have a large incubator for long term live cell imaging.

Click on the microscope names to find out details about each system.

Tweety

This system can be used as:

- Single point scanning confocal 

- Resonant scanner for fast imaging with large field of view (1024x1024 pixels)

- Spinning disk CREST v3

- Ultra fast widefield with large field of view 

- Micropatterning (printing proteins on a dish) and micromanufacturing (making microwells)

Equipment specifications:

- Nikon

- Inverted

- Full size incubator for life cell imaging

- Fully motorized including Z piezo 100 um

- High content screening JOBS module

- 2 very sensitive cameras from Photometrics: Prime 95B sCMOS, 95% QE, 11um pixels for best sensitivity, 1608x1608 pixels and BSIexpress sCMOS, 95% QE, 6.5 um for best resolution, 2048x2048 pixels  

- Alvéole Primo micropatterning and micromanufacturing device

Filters for Widefield and Spinning disk side:

Lasers:  405, 446, 477, 520, 546, 638, 776 (nm)

Dichroic mirrors: 441/30; 511/26; 593/37; 684/34; 817/66 (nm)

Dichroic mirrors: 484/22; 561/30

Emission filters:  

- Pentaband: 441/30; 511/26; 593/37; 684/34; 817/66 (nm)

- Dual band: 484/20; 561/30 (nm)

- Single bands: 438/24; 485/20; 511/20; 560/25; 595/31; 685/40; 819/44 (nm)

 

Filters, laser and dichroic mirrors on the confocal side:

Lasers (diode): 405, 488, 561; 640 (nm)

Dichroic mirrors: 405/488 ; 405/488/561; 405/488/561/640; BS 20/80 (nm)

Emission filters: 450/50; 525/50; 595/50; 700/75 (nm)

 

 

Pegasus

This system can be used as:

- Single point scanning confocal

Equipment specifications:

- Nikon

- Inverted

- Full size incubator for life cell imaging

- Fully motorized including Z piezo 100 um

- High content screening JOBS module

- Resonant scanner for fast imaging

- 2x GaAsP detectors (green and red)

Filters, lasers and dichroic mirrors:

Lasers:  405 (diode), 457/488/514 (Argon Ion) 561 (diode); 640 (diode) (nm)

Dichroic mirrors: 405/488; 405/488/561; 405/488/561/640; 405-457/514; 457/514/561; BS20/80 (nm)

Emission filters: 450/50;482/35; 525/50; 540/30; 595/50; 700/75 (nm)

 

 

Dragon

This system can be used as:

- Single point scanning confocal

Equipment specifications:

- Nikon

- Inverted

- Full size incubator for life cell imaging

- Fully motorized

- High content screening JOBS module

- Resonant scanner for fast imaging

- 2x GaAsP detectors (green and red)

- 32-channel spectral detector

Filters, lasers and dichroic mirrors:

Lasers:  405 (diode), 457/488/514 (Argon Ion) 561 (diode); 640 (diode) (nm)

Dichroic mirrors: 405/488; 405/488/561; 405/488/561/640; 405-457/514; 457/514/561; BS20/80 (nm)

Emission filters: 450/50; 482/35; 525/50; 540/30; 595/50; 700/75 (nm)

 

 

Orion

Sylvie Le Guyader and Tobias Nyberg working with Orion

This system can be used as:

- Single point scanning confoca

- Widefield

- TIRF

- STORM super resolution

Equipment specifications:

- Nikon

- Inverted

- Full size incubator for life cell imaging

- Fully motorized including Z piezo 100 um

- High content screening JOBS module

- 32-channel spectral detector

- Andor EM-CCD camera, 60 fps at 512x512 pixels. Pixel size 16 um. QE 95%

 

Filters on the widefield side:

Excitation filters:  387/11; 472/30; 562/40; 620/60 (nm)

Dichroic mirrors: 409LP; 495LP; 593LP; 660LP (nm)

Emission filters:  447/60; 520/35; 624/40; 700/75 (nm)

 

Filters, lasers and dichroic mirrors for the confocal:

Lasers:  405 (diode), 457/488/514 (Argon Ion) 561 (diode); 647 (diode) (nm)

Dichroic mirrors: 405/488 ; 405/488/561; 405/488/561/640; 405-457/514 ; 457/514/561; BS20/80 (nm)

Emission filters: 450/50; 482/35; 525/50; 540/30; 595/50; 700/75 (nm)

Filter cubes for STORM:

STORM cube: 405; 488; 561; 647,  (5 nm width). Emission filter: 705/70 nm.

Filter cubes for TIRF:

Dual TIRF: Excitation filters:  488/561 (5 nm width); DM: 507/24; 565LP (emission) (nm); Emission filters: 522/45; 570LP (nm)

Quad TIRF: Excitation filters; 405; 488; 561; 647, (5 nm width); DM: 450/60; 525/60; 597/55; 607/55: 670LP (emission); Emission Filters: 451/29; 525/50; 607/18; 675LP (nm)

 

 

Victor

This system can be used as:

- Spinning disk confocal

- Ultra fast widefield

- TIRF

Equipment specifications:

- Nikon

- Inverted

- Full size incubator for life cell imaging

- Fully motorized including Z piezo 250 um

- High content screening JOBS module

- Widefield: Andor camera Zyla 4.2+. 100 fps at 2048x2048 pixels. Pixel size 6.45 um. QE 82%

- External filter wheel and SpectraX LED for fast imaging

- Spinning disk: Andor EM-CCD camera, 60 fps at 512x512 pixels. Pixel size 16 um. QE 95%

- Spinning disk Yokagawa CSU-X1, 10,000 rpm

 

Filters on the spinning disk confocal side:

Lasers:  405, 488; 561; 640 (nm)

Dichroic mirrors: 431/44; 519/52; 599/55; 652LP (emission) (nm)

Emission filters: 447/60; 520/30 ; 607/36; 685/40; Dual band: 512/30; 630/100; Triple band: 525 600 690 (nm)

 

Filters on the widefield side:

Excitation filters:  395/25, 440/20; 475/28; 510/25; 550/15; 575/25; 631/28 (nm)

Dichroic mirrors WF: 452/55; 529/52; 604/48; 675LP (emission) (nm)

Dichroic mirror CFP/YFP FRET: 458LP (emission) (nm)

Emission filters: 447/60; 483/32 ; 500LP; 525/30; 525/30; 542/27; 607/36; 630/92; 685/40 (nm)

 

Filters on the TIRF side:

Dual TIRF: Excitation filters:  488/561 (5 nm width); DM: 520/50; 565LP (emission) (nm); Emission filters: 522/45; 570LP (nm)

Quad TIRF: Excitation filters; 405; 488; 561; 647, 5 nm width; DM: 450/60; 525/60; 597/55; 607/55: 670LP (emission); Emission Filters: 451/29; 525/50; 607/18; 675LP (nm)

 

 

Zeus

 

This system can be used as:

- Single point scanning confocal 

- Multiphoton point scanning confocal

Equipment specifications:

- Zeiss LSM710-NLO

- Upright

- Full size incubator for life cell imaging

- Fully motorized

- 34-channel spectral detector

- GaAsP detector

Lasers: 405 (diode); 457/488/514 Argon; 561 (diode); 633 (diode)

-  4 NDDs (2 trans and 2 epi) (specifications, in the table below):

Fluorophore Dichroic mirror Emission
Blue Mirror SP 485
Green LP 490 BP 500-550 used in combination with the blue filter cube
Green mirror mirror BP 500-550 used in combination with the red filter cube
Red LP 555 BP 565-610

Aurora 

This system can be used as:

- Light sheet microscope

 

Equipment specifications:

-M Squared Lasers

-Upright

-Full size incubator for life cell imaging

-Fully motorized including xy scanning size of a multiwell plate

-Z fast beam scanning 900 um, additional stage scanning 1 cm

- No shadowing effect thanks to the self-healing Airy beam

- < 1um xy and z resolution with a field of view of 870x870 um

-Tiling/stitching, multiple sample or regions

-Dedicated deconvolution software running on GPU power

-Camera: Hamamatsu Orca Flash 4 V.3 100 fps at 2048x2048 pixels. Pixel size 6.45 um. QE 82%

-Refraction index: 1.33-1.56 (no organic solvents) without changing objectives

-Objective: Navitar multi-immersion: 15.3x – 17.9x; NA 0.37-0.43.

- Working distance 12mm

How to calculate the objective NA and magnification:
- NA=0.276xRI
- Mag=11.494xRI
- WD = constant for all RI

Lasers (mW): 405nm(60 mW); 457nm(100 mW); 488nm(100 mW); 515nm(100 mW); 561nm(80 mW); 642nm(140 mW); 685nm(50 mW)

Filters: 445BP58; 525/50; 605/52; 667/30; 700LP (nm)

For more information:  Aurora-light sheet microscopes.

A powerful server is available for fast transfer/storage/deconvolution/stitching/analysis.

 

 

Minerva

This system can be used as:

- Widefield

- Slide scanner

- Laser microdissection for RNA and protein analysis of part of a tissue

- Cell picker and sorter

Equipment specifications:

- Nikon

- Inverted

- Fully motorized

- High content screening JOBS module

- Nikon colour camera

- Andor EM-CCD camera, 60 fps at 512x512 pixels. Pixel size 16 um. QE 95%

- MMI CellCut and CellEctor

 

Filters for widefield:

Excitation filters: 365/10; 470/40; 544/25; 628/40 (nm)

Dichroic mirrors: 400LP; 495LP; 565LP; 660LP (nm)

Emission filters: 460/50; 515/30; 605/70; 692/40 (nm)