Microscopes

  • 1 Zeiss single and multiphoton confocal (Zeus)
  • 4 Nikon confocals (Orion, Tweety, Dragon, Pegasus)
  • 2 Nikon spinning disk confocals (Victor and Tweety)
  • 4 Nikon widefield microscopes (Victor, Orion, Tweety, Minerva)
  • 2 Nikon TIRF microscopes (Orion, Victor)
  • 1 Nikon STORM super resolution microscope (Orion)
  • 1 M-Squared Light Sheet Microscope (Aurora)
  • Nikon JOBS High content screening module (Dragon, Tweety, Orion, Pegasus, Victor, Minerva)
  • 1 Primo (Alveole) micropatterning and micromanufacturing device (Tweety)
  • 1 MMI laser capture microdissection and cell picking system (Minerva)
  • 1 MMI Slide scanner with a colour camera (Minerva)
  • Imaris, NIS-Element, Zen, Fiji, Cell Profiler, Deconvolution, ImSpector, MatLab, Python, CTFire, Omero

Some of our confocals are equipped with fast resonant scanners, and spectral detectors.
Most of our microscopes have a large incubator for long term live cell imaging.

Click on the microscope names to find out details about each system.

Tweety

 

This system can be used as:

- Single point scanning confocal 

- Resonant scanner for fast imaging with large field of view (1024x1024 pixels)

- Spinning disk CREST v3

- Ultra fast widefield with large field of view 

- Micropatterning (printing proteins on a dish) and micromanufacturing (making microwells)

Equipment specifications:

- Nikon

- Inverted

- Fully motorized including Z piezo 100 um

- High content screening JOBS module

- 2 very sensitive cameras from Photometrics: Prime 95B sCMOS, 95% QE, 11um pixels for best sensitivity and 1608x1608 large field of view and BSI sCMOS, 95% QE,  6.45 um for best resolution and 2048x2048 field of view  

- Alvéole Primo micropatterning and micromanufacturing device

 

Filters for Widefield and Spinning disk side

Lasers:  405, 446, 477, 520, 546, 638, 749

Dichroic mirrors: 421/491/567/639/776;  471/539

Emission filters:  

- Pentaband: 441/30; 511/26; 593/37; 684/34; 817/66;

- Dual band: 484/20; 561/30

- Single bands:438/24; 485/20; 511/20; 560/25; 595/31; 685/40; 819/44

 

Filters, laser and dichroic mirrors on the confocal side:

Lasers (diode): 405, 488, 561; 640

Dichroic mirrors: 405/488 ; 405/488/561; 405/488/561/640; BS 20/80

Emission filters: 450/50; 525/50; 595/50; 700/75

 

 

Pegasus

 

This system can be used as:

- Single point scanning confocal

Equipment specifications:

- Nikon

- Inverted

- Full size incubator for life cell imaging

- Fully motorized including Z piezo 100 um

- High content screening JOBS module

- Resonant scanner for fast imaging

- 2x GaAsP detectors (green and red)

 

Filters, lasers and dichroic mirrors:

Lasers:  405 (diode), 457/488/514 (Argon Ion) 561 (diode); 640 (diode)

Dichroic mirrors: 405/488 ; 405/488/561; 405/488/561/640; 405-457/514 ; 457/514/561; BS20/80

Emission filters: 450/50;482/35 ; 525/50 ; 540/30; 595/50; 700/75

 

 

 

Dragon

 

This system can be used as:

- Single point scanning confocal

Equipment specifications:

- Nikon

- Inverted

- Full size incubator for life cell imaging

- Fully motorized

- High content screening JOBS module

- Resonant scanner for fast imaging

- 2x GaAsP detectors (green and red)

- 32-channel spectral detector

 

Filters, lasers and dichroic mirrors:

Lasers:  405 (diode), 457/488/514 (Argon Ion) 561 (diode); 640 (diode)

Dichroic mirrors: 405/488 ; 405/488/561; 405/488/561/640; 405-457/514 ; 457/514/561; BS20/80

Emission filters: 450/50;482/35 ; 525/50 ; 540/30; 595/50; 700/75

 

 

Orion

 

 

This system can be used as:

- Single point scanning confoca

- Widefield

- TIRF

- STORM super resolution

Equipment specifications:

- Nikon

- Inverted

- Full size incubator for life cell imaging

- Fully motorized including Z piezo 100 um

- High content screening JOBS module

- 32-channel spectral detector

- Andor EM-CCD camera, 60 fps at 512x512 pixels. Pixel size 16 um. QE 95%

Filters on the widefield side:

Cube Excitation Filters Dichroic Filters Emission Filters
Blue 387/11 409LP 447/60
Green 472/30 495LP 520/35
Red 562/40 593LP 624/40
Far Red 620/60 660LP 700/75

Filters, lasers and dichroic mirrors for the confocal:

Lasers:  405 (diode), 457/488/514 (Argon Ion) 561 (diode); 640 (diode)

Dichroic mirrors: 405/488 ; 405/488/561; 405/488/561/640; 405-457/514 ; 457/514/561; BS20/80

Emission filters: 450/50;482/35 ; 525/50 ; 540/30; 595/50; 700/75

Filter cubes for TIRF/STORM:

STORM cube: 405; 488; 561; 647; 5 nm width. Emission filter: 670-740 nm.

Cube Excitation Filters Dichroic Filters Emission Filters
Dual TIRF 480-495; 555-565 495-545; 565LP 500-545; 570LP
Quad TIRF 385-418 420-480 422-480
485-495 495-555 500-550
555-570 575-630 580-625
635-660 670LP 675LP

Victor

 

This system can be used as:

- Spinning disk confocal

- Ultra fast widefield

- TIRF

Equipment specifications:

- Nikon

- Inverted

- Full size incubator for life cell imaging

- Fully motorized including Z piezo 250 um

-Hgh content screening JOBS module

- Wideld: Andor camera Zyla 4.2+. 100 fps at 2048x2048 pixels. Pixel size 6.45 um. QE 82%

- External filter wheel and SpectraX LED for fast imaging

- Spinning disk: Andor EM-CCD camera, 60 fps at 512x512 pixels. Pixel size 16 um. QE 95%

- Spinning disk Yokagawa CSU-X1, 10,000 rpm

Filters on the spinning disk confocal side:

Lasers Quad Dichroic Mirror Emission Filters
405 nm diode 409-453; 493-545; 573-626; 652LP 447/60
488 nm diode 520/30
512/30; 630/100
561 nm diode 607/36
640 nm diode 685/40
525 600 690

Filters on the widefield/TIRF side:

 

LEDs Dichroic Mirrors Emission Filters
395/25 nm 425-480 447/60
440/20 nm 503-555 483/32
475/28 nm 580-628 500LP
510/25 nm 675LP 525/30
550/15 nm (or 575/25) 542/27
631/28 nm 607/36
630/92
685/40

Zeus

 

This system can be used as:

- Single point scanning confocal 

- Multiphoton point scanning confocal

Equipment specifications:

- Zeiss LSM710-NLO

- Upright

- Full size incubator for life cell imaging

- Fully motorized

- 34-channel spectral detector

- GaAsP detector

Lasers

- 405 diode

- 457/488/514 Argon

- 561 diode

- 633 diode

- 4 NDDs (2 trans and 2 epi) (specifications, in the table below)

Fluorophore Dichroic mirror Emission
Blue Mirror SP 485
Green LP 490 BP 500-550 used in combination with the blue filter cube
Green mirror mirror BP 500-550 used in combination with the red filter cube
Red LP 555 BP 565-610

Aurora 

This system can be used as:

- Light sheet microscope

 

Equipment specifications:

-M Squared Lasers

-Upright

-Full size incubator for life cell imaging

-Fully motorized including xy scanning size of a multiwell plate

-Z fast beam scanning 900 um, additional stage scanning 1 cm

- No shadowing effect thanks to the self-healing Airy beam

- < 1um xy and z resolution with a field of view of 870x870 um

-Tiling/stitching, multiple sample or regions

-Dedicated deconvolution software running on GPU power

-Camera: Hamamatsu Orca Flash 4 V.3 100 fps at 2048x2048 pixels. Pixel size 6.45 um. QE 82%

-Refraction index: 1.33-1.56 (no organic solvents) without changing objectives

-Objective: Navitar multi-immersion: 15.3x – 17.9x; NA 0.37-0.43.

- Working distance 12mm

 

Lasers (mW): 405nm(60 mW); 457nm(100 mW); 488nm(100 mW); 515nm(100 mW); 561nm(80 mW); 642nm(140 mW); 685nm(50 mW)

Filters: 445BP58; 525/50; 605/52; 667/30; 700LP

For more information:  Aurora-light sheet microscopes.

A powerful server is available for fast transfer/storage/deconvolution/stitching/analysis.

 

 

Minerva

This system can be used as:

- Widefield

- Slide scanner

- Laser microdissection for RNA and protein analysis of part of a tissue

- Cell picker and sorter

Equipment specifications:

- Nikon

- Inverted

- Fully motorized

- High content screening JOBS module

- Nikon colour camera

- Andor EM-CCD camera, 60 fps at 512x512 pixels. Pixel size 16 um. QE 95%

- MMI CellCut and CellEctor

Filters for widefield

Cube Excitation Filters Dichroic Mirrors Emission Filters
Blue 360-370 400LP 435-485
Green 450-490 495LP 500-530
Red 532-557 565LP 570-640
Far Red 608-648 660LP 672-712