Microscopes
- 1 Zeiss single and multiphoton confocal (Zeus)
- 4 Nikon confocals (Orion, Tweety, Dragon, Pegasus)
- 3 Nikon spinning disk confocals (Victor, Orion and Tweety)
- 5 Nikon widefield microscopes (Victor, Orion, Tweety, Pegasus, Minerva)
- 2 Nikon TIRF microscopes (Orion, Victor)
- 1 Nikon STORM super resolution microscope (Pegasus)
- 1 Nikon DeepSIM super resolution (Orion)
- 1 M-Squared Light Sheet Microscope (Aurora)
- Nikon JOBS High content screening module (Dragon, Tweety, Orion, Pegasus, Victor, Minerva)
- 1 Primo (Alveole) micropatterning and micromanufacturing device (Tweety)
- 1 MMI laser capture microdissection and cell picking system (Minerva)
- 1 MMI Slide scanner with a colour camera (Minerva)
- Imaris, NIS-Element, Zen, Fiji, Cell Profiler, Deconvolution, ImSpector, MatLab, Python, CTFire, Omero
Some of our confocals are equipped with fast resonant scanners, and spectral detectors.
Most of our microscopes have a large incubator for long term live cell imaging.
Click on the microscope names to find out details about each system.
Tweety
This system can be used as:
- Spinning disk CREST v3
- Ultra fast widefield with large field of view
- Micropatterning (printing proteins on a dish) and micromanufacturing (making microwells)
Equipment specifications:
- Nikon
- Inverted
- Full size incubator for life cell imaging
- Fully motorized including Z piezo 100 um
- High content screening JOBS module
- 2 very sensitive cameras from Photometrics: Prime 95B sCMOS, 95% QE, 11um pixels for best sensitivity, 1608x1608 pixels and Kinetix sCMOS, >95% QE, 6.5 um for best resolution, 2720x2720 pixels
- Alvéole Primo micropatterning and micromanufacturing device
Filters for Widefield and Spinning disk side:
Lasers: 405, 446, 477, 520, 546, 638, 749 (nm)
Dichroic mirrors: 441/30; 511/26; 593/37; 684/34; 817/66 (nm)
Dichroic mirrors: 484/22; 561/30
Dichroic mirror: 562LP
Emission filters:
- Pentaband: 441/30; 511/26; 593/37; 684/34; 817/66 (nm)
- Dual band: 484/20; 561/30 (nm)
- Single bands: 438/24; 485/20; 511/20; 560/25; 595/31; 685/40; 561LP (nm); 819/44nm
Filters, laser and dichroic mirrors on the confocal side:
Lasers (diode): 405, 488, 561; 640 (nm)
Dichroic mirrors: 405/488 ; 405/488/561; 405/488/561/640; BS 20/80 (nm)
Emission filters: 450/50; 525/50; 595/50; 700/75 (nm)
Tweety widefield and spinning disk: Semrock searchlight link
Tweety single point confocal: Semrock searchlight link
Pegasus
This system can be used as:
- Single point scanning confocal
- Resonant scanner for fast imaging
- Widefield
- TIRF
- STORM super resolution
Equipment specifications:
- Nikon
- Inverted
- Full size incubator for life cell imaging
- Fully motorized including Z piezo 100 um
- High content screening JOBS module
- 2x GaAsP detectors (green and red)
- 32-channel spectral detector
- Andor EM-CCD camera, 60 fps at 512x512 pixels. Pixel size 16 um. QE 95%
Filters on the widefield side:
Excitation filters: 387/11; 472/30; 562/40; 620/60 (nm)
Dichroic mirrors: 409LP; 495LP; 593LP; 660LP (nm)
Emission filters: 447/60; 520/35; 624/40; 700/75 (nm)
Filters, lasers and dichroic mirrors for the confocal:
Lasers: 405 (diode), 457/488/514 (Argon Ion) 561 (diode); 647 (diode) (nm)
Dichroic mirrors: 405/488 ; 405/488/561; 405/488/561/640; 405-457/514 ; 457/514/561; BS20/80 (nm)
Emission filters: 450/50; 482/35; 525/50; 540/30; 595/50; 700/75 (nm)
Filters, lasers and dichroic mirrors:
Lasers: 405 (diode), 457/488/514 (Argon Ion) 561 (diode); 640 (diode) (nm)
Dichroic mirrors: 405/488; 405/488/561; 405/488/561/640; 405-457/514; 457/514/561; BS20/80 (nm)
Emission filters: 450/50;482/35; 525/50; 540/30; 595/50; 700/75 (nm)
Filter cubes for STORM:
STORM cube: 405; 488; 561; 647, (5 nm width). Emission filter: 705/70 nm.
Filter cubes for TIRF:
Dual TIRF: Excitation filters: 488/561 (5 nm width); DM: 507/24; 565LP (emission) (nm); Emission filters: 522/45; 570LP (nm)
Quad TIRF: Excitation filters; 405; 488; 561; 647, (5 nm width); DM: 450/60; 525/60; 597/55; 607/55: 670LP (emission); Emission Filters: 451/29; 525/50; 607/18; 675LP (nm)
Pegasus widefield: Semrock searchlight link
Pegasus single point confocal: Semrock searchlight link
Dragon
This system can be used as:
- Single point scanning confocal
- Resonant scanner for fast imaging with large field of view (1024x1024 pixels)
Equipment specifications:
- Nikon
- Inverted
- Full size incubator for life cell imaging
- Fully motorized
- High content screening JOBS module
- 2x GaAsP detectors (green and red)
- 32-channel spectral detector
Filters, lasers and dichroic mirrors:
Lasers: 405 (diode), 457/488/514 (Argon Ion) 561 (diode); 640 (diode) (nm)
Dichroic mirrors: 405/488; 405/488/561; 405/488/561/640; 405-457/514; 457/514/561; BS20/80 (nm)
Emission filters: 450/50; 482/35; 525/50; 540/30; 595/50; 700/75 (nm)
Dragon single point confocal: Semrock searchlight link
Orion
This system can be used as:
- Spinning disk CREST v3
- Ultra fast widefield with large field of view
- Super resolution (DeepSIM) ( aproximattely 100nm resolution in xy and aproximattely 300nm resolution in z (60x or100x oil objective); 1024x1024 FOV
Equipment specifications:
- Nikon
- Inverted
- Fully motorized including Z piezo 100 um
- High content screening JOBS module
- Camera from Photometrics, Kinetix sCMOS, >95% QE, 6.5 um for best resolution, 2720x2720 pixels
Filters, lasers and dichroic mirrors:
Lasers: 405, 470, 555, 640, 730 (nm)
Dichroic mirrors: 430/50; 498/55; 613/55; 680/40; 804/90 (nm)
Emission filters:
- Single bands:431/28; 510/50; 580/60; 605/70; 680/30; 811/80 (nm)
- Pentabands: 430/40; 510/60; 567/85; 677/35; 805/90 (nm)
Victor
This system can be used as:
- Spinning disk confocal
- Ultra fast widefield
- TIRF
Equipment specifications:
- Nikon
- Inverted
- Full size incubator for life cell imaging
- Fully motorized including Z piezo 250 um
- High content screening JOBS module
- Widefield: Andor camera Zyla 4.2+. 100 fps at 2048x2048 pixels. Pixel size 6.45 um. QE 82%
- External filter wheel and SpectraX LED for fast imaging
- Spinning disk: Kinetix sCMOS, >95% QE, 6.5 um for best resolution, 1193x1193 pixels
- Spinning disk Yokagawa CSU-X1, 10,000 rpm
Filters on the spinning disk confocal side:
Lasers: 405, 488; 561; 640 (nm)
Dichroic mirrors: 431/44; 519/52; 599/55; 652LP (emission) (nm)
Emission filters: 447/60; 525/30 ; 607/36; 685/40; Dual band: 512/30; 630/100; Triple band: 525 600 690 (nm)
Filters on the widefield side:
Excitation filters: 395/25, 440/20; 475/28; 510/25; 550/15; 575/25; 631/28 (nm)
Dichroic mirrors WF: 452/55; 529/52; 604/48; 675LP (emission) (nm)
Dichroic mirror CFP/YFP FRET: 458LP (emission) (nm)
Emission filters: 447/60; 483/32 ; 500LP; 525/30; 542/27; 572/15; 607/36; 624/40; 630/92; 685/40 (nm)
Filters on the TIRF side:
Dual TIRF: Excitation filters: 488/561 (5 nm width); DM: 520/50; 565LP (emission) (nm); Emission filters: 522/45; 570LP (nm)
Quad TIRF: Excitation filters; 405; 488; 561; 647, 5 nm width; DM: 450/60; 525/60; 597/55; 607/55: 670LP (emission); Emission Filters: 451/29; 525/50; 607/18; 675LP (nm)
Victor widefield: Semrock searchlight link
Victor spinning disk: Semrock searchlight link
Zeus
This system can be used as:
- Single point scanning confocal
- Multiphoton point scanning confocal
Equipment specifications:
- Zeiss LSM710-NLO
- Upright
- Full size incubator for life cell imaging
- Fully motorized
- 34-channel spectral detector
- GaAsP detector
Lasers: 405 (diode); 457/488/514 Argon; 561 (diode); 633 (diode)
- 4 NDDs (2 trans and 2 epi) (specifications, in the table below)
Zeus single and multipoint confocal: Semrock searchlight link
| Fluorophore | Dichroic mirror | Emission | |
|---|---|---|---|
| Blue | Mirror | SP 485 | |
| Green | LP 490 | BP 500-550 | used in combination with the blue filter cube |
| Green mirror | mirror | BP 500-550 | used in combination with the red filter cube |
| Red | LP 555 | BP 565-610 |
Aurora
This system can be used as:
- Light sheet microscope
Equipment specifications:
-M Squared Lasers
-Upright
-Full size incubator for life cell imaging
-Fully motorized including xy scanning size of a multiwell plate
-Z fast beam scanning 900 um, additional stage scanning 1 cm
- No shadowing effect thanks to the self-healing Airy beam
- < 1um xy and z resolution with a field of view of 870x870 um
-Tiling/stitching, multiple sample or regions
-Dedicated deconvolution software running on GPU power
- 2 Hamamatsu Orca Flash cameras 4 V.3 100 fps at 2048x2048 pixels. Pixel size 6.45 um. QE 82%
- Objective: Navitar multi-immersion: 15.3x – 17.9x; NA 0.37-0.43.
- Refraction index: 1.33-1.56 without changing objectives
- All immersion media can be used for imaging
- Working distance 12mm
How to calculate the objective NA and magnification:
- NA=0.276xRI
- Mag=11.494xRI
- WD = constant for all RI
Lasers (mW): 405nm(60 mW); 457nm(100 mW); 488nm(100 mW); 515nm(100 mW); 561nm(80 mW); 642nm(140 mW); 685nm(50 mW)
Filters: 445/58; 480/30; 525/50; 535/30; 590LP; 605/52; 665LP; 667/30; 700LP (nm)
DM: 510 LP (CFP-YFP) and 560 LP
For more information: Aurora-light sheet microscopes.
A powerful server is available for fast transfer/storage/deconvolution/stitching/analysis.
Aurora light sheet: Semrock searchlight link
Minerva
This system can be used as:
- Widefield
- Slide scanner
- Laser microdissection for RNA and protein analysis of part of a tissue
- Cell picker and sorter
Equipment specifications:
- Nikon
- Inverted
- Fully motorized
- High content screening JOBS module
- Nikon colour camera
- Andor EM-CCD camera, 60 fps at 512x512 pixels. Pixel size 16 um. QE 95%
- MMI CellCut and CellEctor
Filters for widefield:
Excitation filters: 365/10; 470/40; 544/25; 628/40 (nm)
Dichroic mirrors: 400LP; 495LP; 565LP; 660LP (nm)
Emission filters: 460/50; 515/30; 605/70; 692/40 (nm)
Nikon AX single point confocal
This system can be used as:
- Single point confocal
- 25 mm FOV resonant scanner and 25 mm FOV galvano scanner
Equipment specifications:
- Nikon, Ti2
- Inverted
- Fully motorized including Z piezo 100 um
- 2 low noise GaAsP and 2 Multi-alkali PMT detectors
- Extremely highspeed imaging (up to 720 fps at 2048 x 16 pixels)
- Spectral detector in up to 66 emission channels for unmixing
- High content screening JOBS module
- Full size incubator for life cell imaging
Filters, laser and dichroic mirrors on the confocal side:
Lasers: 405, 488, 561, 640 (nm)
Dichroic mirrors: 405/488/561/640; BS20/80 (nm)
Emission filters: fixed width: 429-474 and 663-728 and 2 tunable filters