LCI teaching material and videos

Live Cell Imaging Core Facility instruction videos and tutorials.

LCI YouTube channel

Nikon E-Learning platform

Is a web-based learning platform with interactive tutorials on a variety of topics ranging from basic to advanced. To request access, please fill in a request form that you can find on the Nikon website.

After you received the login confirmation, go to the Nikon E-learning platform to access the videos. Here is an introductory video that will help you navigate on the learning platform.

LCI tips

Here you can find all the microscopy tips we send out to our users.

Imaging efficiency and bleedthrough forms

Imaging efficiency and bleedthrough Pegasus (File, 34.3 KB)

Imaging efficiency and bleedthrough Dragon (File, 34.97 KB)

Imaging efficiency and bleedthrough NSPARC (Dragon) (File, 34.74 KB)

Imaging efficiency and bleedthrough Victor - widefield excitation and emission (File, 35.3 KB)

Imaging efficiency and bleedthrough form: Victor multipoint confocal - excitation and emission (File, 34.52 KB)

Imaging efficiency and bleedthrough Tweety - WF and Multipoint confocal excitation and emission (File, 31.63 KB)

Imaging efficiency and bleedthrough form - Aurora (light sheet) (File, 35.11 KB)

Imaging efficiency and bleedthrough form Orion - Widefield and Multipoint confocal and DeepSIM (File, 31.19 KB)

Imaging efficiency and bleedthrough form - Template (File, 34.31 KB)

LCI videos

Imaging efficiency and bleedthrough

Imaging efficiency and bleedthrough Photo: Gabriela Imreh

Links to the bleedthrough video (3 parts):

Imaging efficiency and bleedthrough Part 1 - full: Basic Microscopy

Imaging efficiency and bleedthrough-Part 2-full: Know your sample and your microscope

Imaging efficiency and bleedthrough-Part 3-full: Practical

Basics in Nikon NIS-Elements for confocal microscopy

Introduction to image analysis using Nikon NIS-Elements as an example

Bit depth, saturation and underexposure

Sample preparation tips

How to adjust the Koehler illumination of a microscope (using Nikon Ti as an example)

How to find your sample without bleeching it (using a NIKON microscope)

How to clean coverslip for immunostaining

Removing coverslips from a 24 well plate

How to attach a coverslip to a slide before immunostaining

How to create a tiled (large) image from a custom position

How to use the "Focus surface" function in NIS elements

How to create a tiled (large) image of a sample with unusual shape ments

Efficient strategies to find the area of interest large FOV, tiling and autofocus

How to place a tissue section on a thin coverslip