IncuCyte live cell analysis system

Room nr: U230 05 2510 

Responsible persons: Paula Mannström   Katja Pokrovskaja

Booking calendar: The booking and charging are done in iLab booking system iLab Organizer :: OncPat (corefacilities.org)

Instrument

The instrument is located in a shared cell lab, in the lower left incubator with the Control unit placed on top of the upper left incubator. The plates at positions 1-5 can be used for maximum 1 week. Plate at position 6 can be used for maximum 1 hour. Plates waiting for the scan or removed from the IncuCyte after the end of the experiment should be temporarily stored in the lower right incubator. The available software modules are:

• Standard                    - Organoid        (Trial until end of June 2025)
• Image lock                - Neural activity (Trial until end of June 2025)
• Scratch Wound
• Dilution cloning
• Chemotaxis
• Spheroid
• AI scan

New users

This equipment is a core facility for Theme Cancer at BioClinicum and is accessible for authorized persons only. All users should have passed KI´s Laboratory safety introduction: https://staff.ki.se/kis-laboratory-safety-introduction. Send certificate to paula.mannstrom@ki.se

The new users should contact Katja.Pokrovskaja@ki.se to book and receive an introduction to the routines for this facility, a brief training for operating IncuCyte and some instruction material to become an authorized user. Get started with iLab booking system before the introduction. Each user will also become a member of the “GRP_IncuCyte users” Outlook group. The IncuCyte software can be downloaded for free, either from the memory stick or Sartorius home page, see instructions below. 

General Work Rules 

The defined rules and work procedures for using IncuCyte must be strictly followed:

• the instrument is located in a BSL2 cell laboratory. It is mandatory to use the lab coat and gloves and follow the rules for this type of laboratory.
• Restrict usage to one plate per time and per person. To book more than one plate or for more than 1 week in a row you must send a notification to the “GRP_IncuCyte users” group to get a “green light”.
• Bring your own gloves and materials. Do not use anything from the group in the shared lab. 
• Clean up after yourself. Remove your plate and discard it and all the materials in your own lab. 
• Archive and transfer your raw data files after finished experiments either to your own external hard drive or to your personal network drive.
• For data analysis, IncuCyte software can be downloaded for free, see the link below (for PC only). There is also a devoted Data analysis computer | Karolinska Institutet (ki.se) with an installed software in J6:14 U210 06 6220. You book it through ClusterMarket; to get an account, contact Paula.Mannstrom@ki.se
• Delete archived files regularly from the computer! The full hard disk slows down all the operations!

Links

SX5 Live Cell Analysis Instrument | Sartorius

The IncuCyte software download: Incucyte 2024A GUI – Sartorius – Incucyte Downloads App.

Short video to use Wound Maker: How to use WoundMaker™