Which microscope does my experiment require?

Which microscope does my experiment require?

If you want to image fast and have a flat sample (cell monolayer), you probably need a widefield system (Tweety, Victor, Pegasus).

Confocals (point scanning or spinning disks) are ideal to image samples that are 10-100 um thick (Dragon, Pegasus, Tweety, Victor).

For even thicker samples that are transparent or cleared, you will need our light sheet (Aurora) or our multiphoton system (Zeus).

TIRF is used to image the area of the cell immediately in contact with the coverslip (Pegasus, Victor).

If the resolution needed is higher than 200 nm, you will need super resolution STORM (Pegasus); SIM (Orion).

With our micropatterning system, you can attach proteins at the bottom of a dish, a multiwell plate or on a gel, using the pattern of your choice then study their interaction with cells. You can also make custom-designed microwells (Primo on Tweety).

Use our laser-capture microdissection system to cut part of a tissue for RNA analysis or Mass Spectrometry. You can also pick single cells and transfer them to a tube or a dish (Minerva).

Polarization microscopy is used to quantify collagen in tissue (Pegasus).

On most of our systems, you can image live samples for hours/days (Aurora, Pegasus, Victor, Tweety, Zeus)

Running a screen is easy on all our Nikon system thanks to the high content screening JOBS module (Dragon, Orion, Pegasus, Tweety, Victor)

The image analysis software on our VDI or the Remote Desktop servers will help you extract numbers from multidimensional image files: xy, z, multicolour, time...