Confocal Spinning Disk Microscopes (SDM)
BIC facility is equipped with Confocal Spinning Disk Microscopes offering various configurations and capabilities to cover a wide range of imaging needs and research applications.
Nikon CrEST X-Light V3 Spinning Disk
The Nikon CrEST X-Light V3 spinning disk confocal enables fast imaging with confocal resolution, supporting live and fixed tissue samples. The system can detect a wide range of fluorochromes, featuring LED illumination from ultra-violet 340nm to 635nm and laser options from 405nm to near-infrared 748nm (for detection of AF750 or Cy7).
Specifications
- Stage inserts for: slides/multiwell plates/35mm dishes
- Lasers: 405, 446, 477, 518, 545, 637, 748 nm
- LEDs: 340nm, 380nm, and 420-750nm
- Fluorescent channels: from blue (DAPI) to near infra-red (Cy7)
- Objectives: 4x Air, 10x Air, 20x Air, 20x LWD Air, 25x Silicone and 60x Oil
- Acquisition Software: NIS Elements
- Special Features:
- JOBS module for automatization of complex acquisition pipelines
- Possibility of tiling and automatic imaging at several positions (including focus maps and stitching)
- Black climate chamber with temperature, humidity and CO2 controls for live cell imaging
- 20x extra-long working distance objective (WD=2.3 mm)
- fast widefield imaging (including Fura2 Ca+), confocal imaging and/or volumetric (XYZ) imaging
- NIS.ai Artificial Intelligence module for noise removal
Choosing a microscope and where to find it
If you are interested in more details, we have compiled detailed information about our instruments. The information could be useful when choosing between different microscopes.
You are always welcome to contact us if you need further information or guidance among the microscopes.