Microscopy core facility

Room nr: U230 06 2100

Booking calendar: Clustermarket

Work procedures: There are defined rules and work procedures for starting the microscopes, operating them and using the software as well as finishing the work, all of which have to be strictly followed.

General information: The lab is a core facility for Theme Cancer. This lab is accessible for authorized persons to work with light (bright field, phase contrast), epifluorescence and confocal microscopy. New users should contact one of the responsible persons to become an authorized user and to be added to the booking calendar.

Equipment

Zeiss AxioImager M2

for slides
monochrome camera (IF)

Software functions

image quantification
deconvolution

Fluorescence filters: DAPI, GFP, Rhodamine, FITC, Texas Red, Alexa Fluor 568

Responsible persons: Lena Lennartsson and Paula Mannström

Zeiss AxioObserver Z1 (inverted)

for plates AND slides (change mounting frames yourself)
monochrome camera (IF including far red).
color camera (BF)
LED illumination/Colibri 7
optical sectioning/ApoTome
phase contrast

Responsible persons: Lena Lennartsson and Paula Mannström

Zeiss LSM700 Confocal Microscope

The LSM700 confocal microscope can acquire automatic tile/mosaic images of dyes/fluorochromes within the full visible range (blue to far-red). It can also perform spectral separation to separate fluorochromes with overlapping spectra.

Specifications

Stage inserts for: slides and multiwell plates.
Lasers: 405, 488, 555, 639 nm.
Fluorescent channels: from blue (DAPI), Green (GFP), orange-red (Cy3) to far-red (Cy5).
Objectives: 10x Air, 20x Air, 40x Water and 63x Water.
Acquisition Software: Zen Black

Special Features

Motorized scanning stage that accommodates inserts for slides or multiwelll plates.
Possibility of collecting Zstacks, tiles, timelapse and positions.
Lambda mode/Spectral Imaging, useful for applications such as FRET.

Responsible persons: Ophelie Le Chapelain, Francesca Del Gaudio and Pedro Fonseca