Instrument park
J6:20
Room U220 06 3700 (Apparatus)
VERSAmax Plate Reader (K9060495) for colorimetric analysis. Owner: Common equipment OnkPat. Responsible group: Dalianis
Nanodrop 2000 (K9054221) for DNA/RNA measurements. Owner and Responsible group: Dawei Xu.
NanoDrop (K9054572) for DNA/RNA measurements. Owner: Common equipment OnkPat. Responsible group: Dalianis
Qubit 3.0 Fluorometer (K9054575) for DNA/RNA measurements. Manufacturer: Life technologies. Owner: Dalianis. Responsible group: Dalianis. Contact stefan.holzhauser@ki.se
Room U220 06 2700 (HUB)
Shandon Cytospin 4 (K9070502) for spinning down cells in suspension of glass slides. Manufacturer: Thermo electron corporation (Cellab Nordia AB). Served/repaired January 2021. Owner: Göndor. Responsible group: Mohammad Hojat Farsangi
MAGPIX/Luminex XMAP technology (K9054582) for Multiplex antibody-based single-cell assay. Owner: Dalianis. Responsible group: Dalianis. Attention! An introduction from the owner requires to use the equipment. Contact stefan.holzhauser@ki.se
C1000 Touch Thermal cycler PCR machine (K9054850). Owner and Responsible group: Dalianis
Room U220062100A (C Larsson/W-O Lui lab)
Centrifuge (K9061389) Labofuge 400 R Heraeus Instruments, with cooling and a swing rotor, for 50 and 15 ml-tubes.
Thermostat (K9061377) SL Shel Lab, with a rotating system for slides. Owner and Responsible group: W-O Lui.
Room U220064100 (FACS room)
BioPlex 200 HTF System (K9070442; K9070446; K9070443). Luminex XMAP technology. Manufacturer: BioRad; Service agreement will be signed in case there are interested users (Complete Support Plan (fullservicekontrakt) - SEK 41 906 / year; Proactive Plan (ett förebyggande servicebesök per år) - SEK 21 718 / år). Owner: Österborg. Responsible group: Österborg
Room U220 06 3500 (Microscope room)
Stereo microscope Leica MZ6 (K9060676) and Motic inverted microscope AE21 (K9070498). The change of lamp is required and will be done if there are interested users. Owner: Göndor; Responsible group: Göndör. An introduction from the owner requires to use the equipment. Contact mirco.martino@ki.se
J6:30
Passage U230063600
SmartSpecPlus Spectrophotometer (K9060401). Serviced December 2020. Owner: Common equipment. Responsible person: none
Room 230061140
Pre-PCR room to prepare PCR plates. UV3-HEPA PCR cabinet, Owner: Nauclér group; Fridge/Freezer. Attention! Sterile hood is broken at the moment!
J5:30
Room U230052100 (Apparatus room)
Ultracentrifuge Optima LE 80K (K9060629). Manufacturer Beckman Coulter. Service contract/ Service once a year. Owner: Common equipment OnkPat. Responsible person: Paula Mannström
SpeedVac Concentrator, Savant RVT5105 Refrigerated Vapor trap (K9054735; K9054736). Manufacturer Thermo Scientific. Owner: Nordlund. Responsible group: Nordlund group. Attention! A permission from the owner requires to use the equipment. Contact Anderson.ramos@ki.se
Concentrator Plus (K9052738). Manufacturer: Eppendorf. Owner: Nordlund. Responsible group: Nordlund group. Attention! A permission from the owner requires to use the equipment. Contact Anderson.ramos@ki.se
Water baths: A small - 2210 Branson and a large one with different inserts - Grant GLS400. Owner: Common equipment. Responsible person: none
UVC 500 UV crosslinker. Owner: Common equipment OnkPat. Responsible person: none
GEL DRYER: Model 583 – gel dryer using heat and vacuum.
Equipment for EMSA: chamber for the gel running and a gel dryer using hot air. Owner: Common equipment OnkPat. Responsible person: Katja Pokrovskaja.
Sonicators:
Owner: Common equipment OnkPat. Responsible person: none
Sonicator Biorupter TM UCD-200, Diagenode SA (K9061427; K9061428).
Branson SONIFIER 250 (K9060298; K9060227).
Attention! The sonicators can be only used when no other people are present. Use ear protection and put the sign: Sonication is ongoing” on the door outside to prevent people to come in while sonicator is in use.
Room U230052800 (Nister/BTB cell culture room)
Ibidi flow system (number is missing). This is a pump system for the cultivation of for ex. endothelial cells under flow for simulation of blood vessel formation. It can provide continuous unidirectoinal, oscillating and pulsatile flow. The system consists of two main components: The Ibidi Pump (computer-controlled air pump, placed outside the incubator) and the Fluidic Unit (holder for cell media reservoirs and µ-Slide, tubing, and electrically controlled valve set, placed inside the incubator). Owner: Holmgren/Common equipment OnkPat. Responsible group: Holmgren. Attention! An introduction from the owner requires to use the equipment. Contact Yuanyuan Zhang yuanyuan.zhang.2@ki.se
Room U230053700 (apparatus room)
Elispot Reader (K9061498), AID, ELR04, Owner: Mellstedt/Österborg. Responsible group: Mellstedt/Österborg.
Incubator Thermo Scientific, belongs to BioClinicum. Contact Katja Pokrovskaja or Paula Mannström in case you need to use it.
PCR machine Thermal cycler Touchgene, Manufacturer Techne, Owner: Common equipment, Responsible person: none