Specialized Microscopy Systems at BIC

The BIC facility has several specialized microscopy systems that are optimized for various applications.

Evident Alpha3 Light Sheet Microscope, Facility Edition

The light-sheet microscope from Evident (former Olympus) is for whole-tissue imaging using cleared transparent tissue.


  • Laser lines (nm): 405, 488, 561, 638, 730, 785.
  • Objectives: 2X, 4X, 10X, 25X (3.250, 1.625, 0.650, and 0.260 micrometer/pixel resolution). WD = 8 mm for all objective lens.
  • Illumination, 2X, 5X, 10X (9, 4, and 1.5 micrometer optical thickness each)
  • Extra: Tiling, Real-time focus sweeping, Dynamic focus for multi-color imaging, Shading correction. Chamber volume 240 ml
  • Software: QtSPIM.

LaVision Light-Sheet Microscope

The light-sheet microscope from LaVision Biotec (UltraMicroscope II) is for whole tissue imaging using cleared transparent tissue. The microscope is optimized for organic solvent-based clearing method such as 3DISCO (iDisco).


  • Laser lines (nm): 405, 488, 561, 639, 785.
  • Objectives: 2X with 0.63X to 6.3X adjustment (total magnification is 1.26X to 12.6X), WD = approximately 5.6 mm.
  • Extra: Tiling and Dynamic horizontal focus. For long working distance objectives (1.1X, 4X, 12X), please contact the BIC facility.
  • Software: ImSpector and TeraStitcher for stitching.

Zeiss LSM510 NLO Microscope (2-photon)

The Zeiss LSM510 NLO system is built around an fixed upright Axioscope microscope with a motorised x/y-stage.


  • Laser lines (nm): 670-1080, 488, 515, 561, 594, 647.
  • Objectives: 20x Objective, NA=1.0, working distance 8 mm, 40x Objective, NA=0.8, working distance 6 mm,
  • Extra: Coherent Cameleon UltraII, E-phys.
  • Software: Zen black.

Choosing a microscope

If you are interested in more details we have compiled a sheet for you under More information. The information could be useful when choosing between different systems. 

You are always welcome to contact us if you need further information or guidance among the systems.

More Information

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