Specialized Microscopy Systems at BIC

Evident Alpha3 Light Sheet Microscope, Facility Edition

The light-sheet microscope from Evident (former Olympus) is for whole-tissue imaging using cleared transparent tissue.

Specifications:

• Laser lines (nm): 405, 488, 561, 638, 730, 785.
• Objectives: 2X, 4X, 10X, 25X (3.250, 1.625, 0.650, and 0.260 micrometer/pixel resolution). WD = 8 mm for all objective lens.
• Illumination, 2X, 5X, 10X (9, 4, and 1.5 micrometer optical thickness each)
• Extra: Tiling, Real-time focus sweeping, Dynamic focus for multi-color imaging, Shading correction. Chamber volume 240 ml
• Software: QtSPIM.

LaVision Light-Sheet Microscope

The light-sheet microscope from LaVision Biotec (UltraMicroscope II) is for whole tissue imaging using cleared transparent tissue. The microscope is optimized for organic solvent-based clearing method such as 3DISCO (iDisco).

Specifications:

• Laser lines (nm): 405, 488, 561, 639, 785.
• Objectives: 2X with 0.63X to 6.3X adjustment (total magnification is 1.26X to 12.6X), WD = approximately 5.6 mm.
• Extra: Tiling and Dynamic horizontal focus. For long working distance objectives (1.1X, 4X, 12X), please contact the BIC facility.
• Software: ImSpector and TeraStitcher for stitching.

Zeiss LSM510 NLO Microscope (2-photon)

The Zeiss LSM510 NLO system is built around an fixed upright Axioscope microscope with a motorised x/y-stage.

Specifications:

• Laser lines (nm): 670-1080, 488, 515, 561, 594, 647.
• Objectives: 20x Objective, NA=1.0, working distance 8 mm, 40x Objective, NA=0.8, working distance 6 mm,
• Extra: Coherent Cameleon UltraII, E-phys.
• Software: Zen black.