Shigeaki Kanatani

About me
Publications
Grants
Public outreach and news

Research

I am a research engineer working in BIC imaging facility. I develop new 3D
imaging techniques using tissue clearing and light sheet microscopy.
*Major publication of method development*
Three-dimensional single-cell imaging for the analysis of RNA and protein
expression in intact tumour biopsies. [1]
*Tanaka N, *Kanatani S, *Kaczynska D, Fukumoto K, Louhivuori L, Mizutani T,
/et al/
/Nat Biomed Eng 2020 Jun
():/
* co-first author
Whole-tissue biopsy phenotyping of three-dimensional tumours reveals patterns
of cancer heterogeneity. [2]
*Tanaka N, *Kanatani S, Tomer R, Sahlgren C, Kronqvist P, Kaczynska D, /et
al/
/Nat Biomed Eng 2017 Oct
1(10):796-806/
* co-first author
[1] https://pubmed.ncbi.nlm.nih.gov/32601394
[2] https://www.ncbi.nlm.nih.gov/pubmed/31015588

Articles

Article: EXPERIMENTAL EYE RESEARCH. 2023;237:109674
Anatomy of the complete mouse eye vasculature explored by light-sheet fluorescence microscopy exposes subvascular-specific remodeling in development and pathology
Krimpenfort LT; Garcia-Collado M; van Leeuwen T; Locri F; Luik A-L; Queiro-Palou A; Kanatani S; Andre H; Uhlen P; Jakobsson L
Article: NATURE NEUROSCIENCE. 2023;26(6):1008-1020
ARG1-expressing microglia show a distinct molecular signature and modulate postnatal development and function of the mouse brain
Stratoulias V; Ruiz R; Kanatani S; Osman AM; Keane L; Armengol JA; Rodriguez-Moreno A; Murgoci A-N; Garcia-Dominguez I; Alonso-Bellido I; Ibanez FG; Picard K; Vazquez-Cabrera G; Posada-Perez M; Vernoux N; Tejera D; Grabert K; Cheray M; Gonzalez-Rodriguez P; Perez-Villegas EM; Martinez-Gallego I; Lastra-Romero A; Brodin D; Avila-Carino J; Cao Y; Airavaara M; Uhlen P; Heneka MT; Tremblay M-E; Blomgren K; Venero JL; Joseph B
Article: NATURE COMMUNICATIONS. 2023;14(1):3060
Directionality of developing skeletal muscles is set by mechanical forces
Sunadome K; Erickson AGG; Kah D; Fabry B; Adori C; Kameneva P; Faure L; Kanatani S; Kaucka M; Ellstroem ID; Tesarova M; Zikmund T; Kaiser J; Edwards S; Maki K; Adachi T; Yamamoto T; Fried K; Adameyko I
Article: ACTA NEUROPATHOLOGICA. 2022;144(4):651-676
Spatiotemporal characterization of cellular tau pathology in the human locus coeruleus-pericoerulear complex by three-dimensional imaging
Gilvesy A; Husen E; Magloczky Z; Mihaly O; Hortobagyi T; Kanatani S; Heinsen H; Renier N; Hokfelt T; Mulder J; Uhlen M; Kovacs GG; Adori C
Article: NATURE COMMUNICATIONS. 2022;13(1):1537
GIT1 protects against breast cancer growth through negative regulation of Notch
Zhang S; Miyakawa A; Wickstrom M; Dyberg C; Louhivuori L; Varas-Godoy M; Kemppainen K; Kanatani S; Kaczynska D; Ellstrom ID; Elfman L; Kronqvist P; Repo H; Mikoshiba K; Sahlgren C; Johnsen JI; Uhlen P
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2022;119(7):e2108768119
Disrupted Cacna1c gene expression perturbs spontaneous Ca²⁺ activity causing abnormal brain development and increased anxiety
Smedler E; Louhivuori L; Romanov RA; Masini D; Ellstrom ID; Wang C; Caramia M; West Z; Zhang S; Rebellato P; Malmersjo S; Brusini I; Kanatani S; Fisone G; Harkany T; Uhlen P
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2020;117(50):32005-32016
Targeting a scavenger receptor on tumor-associated macrophages activates tumor cell killing by natural killer cells
Eisinger S; Sarhan D; Boura VF; Ibarlucea-Benitez I; Tyystjarvi S; Oliynyk G; Arsenian-Henriksson M; Lane D; Wikstrom SL; Kiessling R; Virgilio T; Gonzalez SF; Kaczynska D; Kanatani S; Daskalaki E; Wheelock CE; Sedimbi S; Chambers BJ; Ravetch JV; Karlsson MCI
Article: EXPERIMENTAL CELL RESEARCH. 2020;395(1):112156
Notch activation in the mouse mammary luminal lineage leads to ductal hyperplasia and altered partitioning of luminal cell subtypes
Phoon YP; Chivukula IV; Tsoi YL; Kanatani S; Uhlen P; Kuiper R; Lendahl U
Article: NATURE BIOMEDICAL ENGINEERING. 2020;4(9):875-888
Three-dimensional single-cell imaging for the analysis of RNA and protein expression in intact tumour biopsies
Tanaka N; Kanatani S; Kaczynska D; Fukumoto K; Louhivuori L; Mizutani T; Kopper O; Kronqvist P; Robertson S; Lindh C; Kis L; Pronk R; Niwa N; Matsumoto K; Oya M; Miyakawa A; Falk A; Hartman J; Sahlgren C; Clevers H; Uhlen P
Article: NEUROSCIENCE. 2019;402:78-89
The T-type Ca²⁺ Channel Ca_v3.2 Regulates Differentiation of Neural Progenitor Cells during Cortical Development via Caspase-3
Rebellato P; Kaczynska A; Kanatani S; Al Rayyes I; Zhang S; Villaescusa C; Falk A; Arenas E; Hermanson O; Louhivuori L; Uhlen P
Journal article: EUROPEAN UROLOGY OPEN SCIENCE. 2019;18(1):e658-e659
499 Transcription and protein phenotyping with 3D pathology: Light-sheet microscopy overlooks cellular malignancy of intact tumour volumes
Tanaka N; Kanatani S; Kaczynska D; Louhivuori L; Oya M; Miyakawa A; Uhlén P
Article: BRITISH JOURNAL OF CANCER. 2018;118(7):995-999
Mapping of the three-dimensional lymphatic microvasculature in bladder tumours using light-sheet microscopy
Tanaka N; Kaczynska D; Kanatani S; Sahlgren C; Mitura P; Stepulak A; Miyakawa A; Wiklund P; Uhlen P
Journal article: EUROPEAN UROLOGY OPEN SCIENCE. 2018;17(2):e312-e315
216 Light-sheet microscopy: Diagnosing intratumoral heterogeneity of intact tumors in three-dimension
Tanaka N; Kanatani S; Kaczynska D; Louhivuori L; Wiklund P; Oya M; Miyakawa A; Uhlén P
Article: NATURE BIOMEDICAL ENGINEERING. 2017;1(10):796-806
Whole-tissue biopsy phenotyping of three-dimensional tumours reveals patterns of cancer heterogeneity
Tanaka N; Kanatani S; Tomer R; Sahlgren C; Kronqvist P; Kaczynska D; Louhivuori L; Kis L; Lindh C; Mitura P; Stepulak A; Corvigno S; Hartman J; Micke P; Mezheyeuski A; Strell C; Carlson JW; Moro CF; Dahlstrand H; Ostman A; Matsumoto K; Wiklund P; Oya M; Miyakawa A; Deisseroth K; Uhlen P
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2015;112(36):E4985-E4994
The COUP-TFII/Neuropilin-2 is a molecular switch steering diencephalon-derived GABAergic neurons in the developing mouse brain
Kanatani S; Honda T; Aramaki M; Hayashi K; Kubo K-I; Ishida M; Tanaka DH; Kawauchi T; Sekine K; Kusuzawa S; Kawasaki T; Hirata T; Tabata H; Uhlen P; Nakajima K
Article: CEREBRAL CORTEX. 2015;25(4):991-1003
Cdk5 Phosphorylation of ErbB4 is Required for Tangential Migration of Cortical Interneurons
Rakic S; Kanatani S; Hunt D; Faux C; Cariboni A; Chiara F; Khan S; Wansbury O; Howard B; Nakajima K; Nikolic M; Parnavelas JG
Article: DEVELOPMENTAL NEUROBIOLOGY. 2015;75(4):360-368
Calcium Signaling in Neocortical Development
Uhlen P; Fritz N; Smedler E; Malmersjoe S; Kanatani S
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2013;110(16):E1524-E1532
Neural progenitors organize in small-world networks to promote cell proliferation
Malmersjo S; Rebellato P; Smedler E; Planert H; Kanatani S; Liste I; Nanou E; Sunner H; Abdelhady S; Zhang S; Andang M; El Manira A; Silberberg G; Arenas E; Uhlen P
Article: EUROPEAN JOURNAL OF NEUROSCIENCE. 2012;36(3):2284-2292
Leucine-rich glioma inactivated 1 (Lgi1), an epilepsy-related secreted protein, has a nuclear localization signal and localizes to both the cytoplasm and the nucleus of the caudal ganglionic eminence neurons
Kusuzawa S; Honda T; Fukata Y; Fukata M; Kanatani S; Tanaka DH; Nakajima K
Article: EUROPEAN JOURNAL OF NEUROSCIENCE. 2012;36(3):2273-2283
Involvement of metabotropic glutamate receptor 5 signaling in activity-related proliferation of adult hippocampal neural stem cells
Nochi R; Kato T; Kaneko J; Itou Y; Kuribayashi H; Fukuda S; Terazono Y; Matani A; Kanatani S; Nakajima K; Hisatsune T
Article: CEREBRAL CORTEX. 2009;19(9):2092-2105
Differences of Migratory Behavior between Direct Progeny of Apical Progenitors and Basal Progenitors in the Developing Cerebral Cortex
Tabata H; Kanatani S; Nakajima K
Article: JOURNAL OF NEUROSCIENCE. 2008;28(50):13582-13591
COUP-TFII Is Preferentially Expressed in the Caudal Ganglionic Eminence and Is Involved in the Caudal Migratory Stream
Kanatani S; Yozu M; Tabata H; Nakajima K
Article: JOURNAL OF NEUROSCIENCE. 2008;28(22):5794-5805
Cell-autonomous roles of ARX in cell proliferation and neuronal migration during corticogenesis
Friocourt G; Kanatani S; Tabata H; Yozu M; Takahashi T; Antypa M; Raguenes O; Chelly J; Ferec C; Nakajima K; Parnavelas JG
Article: CEREBRAL CORTEX. 2005;15(3):332-340
Layer-specific production of nitric oxide during cortical circuit formation in postnatal mouse brain
Imura T; Kanatani S; Fukuda S; Miyamoto Y; Hisatsune T
Journal article: JOURNAL OF CHILD NEUROLOGY. 2004;19(3):274-279
Topical Review: Neuronal Migration in Cortical Development
Kanatani S; Tabata H; Nakajima K
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All other publications

Conference publication: JOURNAL OF BONE AND MINERAL RESEARCH. 2023;38:336
The orientation of developing skeletal muscles is set by mechanical forces
Erickson A; Adameyko I; Sunadome K; Kah D; Fabry B; Adori C; Kanatani S; Kameneva P; Faure L; Kaucka M; Ellstrom ID; Tesarova M; Zikmund T; Kaiser J; Edwards S; Maki K; Adachi T; Fried K
Conference publication: GLIA. 2023;71:E512
ARG1 expression in basal forebrain microglia modulates hippocampal innervation and cognition during postnatal development
Venero JL; Ruiz R; Kanatani S; Osman AM; Keane L; Armengol JA; Rodriguez-Moreno A; Murgoci A-N; Garcia-Dominguez I; Alonso-Bellido I; Ibanez FG; Picard K; Vazquez-Cabrera G; Posada-Perez M; Vernoux N; Tejera D; Grabert K; Cheray M; Gonzalez-Rodriguez P; Perez-Villegas EM; Martinez-Gallego I; Lastra-Romero A; Brodin D; Avila-Carino J; Cao Y; Airavaara M; Uhlen P; Heneka MT; Tremblay M-E; Blomgren K; Stratoulias V; Joseph B
Preprint: BIORXIV. 2022
Whole-Brain Three-Dimensional Imaging of RNAs at Single-Cell Resolution
Kanatani S; Kreutzmann J; Li Y; West Z; Vougesi Nikou D; Lercke Skytte J; Lydolph Larsen L; Tanaka D; Kaczynska D; Fukumoto K; Uesaka N; Tanabe T; Miyakawa A; Roostalu U; Hecksher-Sørensen J; Uhlén P
Preprint: BIORXIV. 2022
Anatomy of the complete mouse eye vasculature in development and pathology explored by light-sheet fluorescence microscopy
Krimpenfort L; Garcia-Collado M; van Leeuwen T; Locri F; Luik A-L; Queiro-Palou A; Kanatani S; André H; Uhlén P; Jakobsson L
Editorial comment: NATURE METHODS. 2022;19(5):527-529
Imaging cleared tissues made easy
Kanatani S; Uhlen P
Preprint: BIORXIV. 2021
Arg1⁺ microglia are critical for shaping cognition in female mice
Stratoulias V; Ruiz R; Kanatani S; Osman A; Armengol J; Rodríguez-Moreno A; Murgoci A-N; García-Domínguez I; Keane L; Vázquez-Cabrera G; Alonso-Bellido I; Vernoux N; Tejera D; Grabert K; Cheray M; González-Rodríguez P; Pérez-Villegas E; Martinez-Gallego I; Brodin D; Avila-Cariño J; Airavaara M; Uhlén P; Heneka M; Tremblay M-È; Blomgren K; Venero J; Joseph B
Editorial comment: NATURE BIOMEDICAL ENGINEERING. 2018;2(10):717-718
Predicting a tumour's drug uptake
Louhivuori L; Kanatani S; Uhlen P
Corrigendum: NATURE BIOMEDICAL ENGINEERING. 2018;2(1):48
Whole-tissue biopsy phenotyping of three-dimensional tumours reveals patterns of cancer heterogeneity (vol 1, pg 796, 2017)
Tanaka N; Kanatani S; Tomer R; Sahlgren C; Kronqvist P; Kaczynska D; Louhivuori L; Kis L; Lindh C; Mitura P; Stepulak A; Corvigno S; Hartman J; Micke P; Mezheyeuski A; Strell C; Carlson JW; Moro CF; Dahlstrand H; Ostman A; Matsumoto K; Wiklund P; Oya M; Miyakawa A; Deisseroth K; Uhlen P
Conference publication: NEUROSCIENCE RESEARCH. 2011;71:E128
Molecular mechanisms involved in the caudal migratory stream of cortical interneurons
Kusuzawa S; Kanatani S; Golden J; Tanaka D; Nakajima K
Published conference paper: JOURNAL OF CHILD NEUROLOGY. 2005;20(4):274-279
Neuronal migration in cortical development
Kanatani S; Tabata H; Nakajima K

Grants

Exclusively a molecule expressed, cooperatively derived inhibitory neurons expressing a specific
Japan Society for the Promotion of Science
1 January 2011 - 31 December 2012
Mechanisms that regulate migrating cell dynamics in the developing brain
Japan Society for the Promotion of Science
1 April 2010 - 31 March 2015
Identification of the factors that define the direction of travel in favor of newly born neurons
Japan Society for the Promotion of Science
1 January 2007 - 31 December 2007

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