Objectives

Objectives that are permanently available on each of our NIKON microscopes

Magnification Immersion n NA WD mm Type
Plan Apo 10x dry 1 0.5 4
Plan Apo 20x dry 1 0.75 1
Plan Apo 60x oil 1.55 1.40 0.13 Correction of chromatic aberrations (405-660 nm)

Objectives that are rotating between our NIKON microscopes

Magnification Immersion n NA WD mm Type
Plan Apo 4x dry 1 0.20 20
PLan Fluor 20x dry 1 0.5 21
Plan Apo 40x dry 1 0.95 0.16-0.23 (ring) Correction for glass thickness (0.11-0.23)
Plan Fluor 40x oil 1.52 1.30 0.2
PLan Fluor 40x dry 1 0.75 0.66
Plan Apo 60x (TIRF) oil 1.52 1.49 0.28-0.31(ring) Correction for glass thickness and temperature (0.13-0.19/23 degrees;0.15-0.21/37 degrees)
Plan Apo 60x water 1.33 1.20 0.12 (ring) Correction for glass thickness (0.15-18); Correction of chromatic aberrations (405-660 nm)
Plan Apo VC 100x (TIRF) oil 1.52 1.49 0.12 (ring) Correction for glass thickness and temperature (0.13-0.19/23 degrees;0.15-0.21/37 degrees
Plan Apo VC 100x oil 1.52 1.40 0.13 Correction of chromatic aberrations (405-660 nm)

Objectives available on Zeus

Magnification Type n NA WD mm
5x Dry 1 0.16 18.5
10x Dry 1 0.45 2.1
20x Dry 1 0.6 1.7
20x Dipping 1.33 1.00 1.8
40x Water 1.33 1.10 0.62
63x Water 1.33 1.20 0.285

Objectives available on Minerva

Magnification Immersion n NA WD mm Type
Plan Fluor PhL 4x dry 1 0.13 16.5
Plan Fluor Ph1 10x dry 1 0.3 15.2
S Plan Fluor Ph1ELWD 20x dry 1 0.45 (ring 8.2-6.9 Correction cover glass thickness (0-2.00)
S Plan Fluor Ph2 ELWD 40x dry 1 0.6 (ring) 3.6-2.8 Correction cover glass thickness (0-2.00)
S Plan Fluor Ph2 ELWD 60x dry 1 0.70 (ring) 2.62-1.8 Correction cover glass thickness (0-2.00)

Objectives available on Tweety

Magnification Immersion n NA WD mm Type
Plan Apo 10x dry 1 0.5 4
Plan Apo 20x dry 1 0.75 1
Plan Apo Lambda Sil 25XC silicon 1.406 1.05 0.55 Correction collar
Plan Apo Lambda Sil 40XC silicon 1.406 1.25 0.3 Correction collar
Apo 60x lambdaS oil 1.52 1.4 0.13