About LCI facility

The Live Cell Imaging Facility is a light microscopy facility at the Karolinska Institute South Campus.

The Live Cell Imaging facility was started in 2008. Since then the facility has grown to include 8 high-end microscopes, 4 experienced staff and a powerful image analysis capability. About 70 researchers actively use the equipment at the LCI facility.

Together our microscopes allow researchers to perform conventional and high throughput confocal and widefield microscopy, fast confocal microscopy (resonance scanner and spinning disk), Fluorescence Life Time Imaging (FLIM), multiphoton microscopy, spectral imaging, super resolution microscopy (TIRF and STORM) and light sheet microscopy. Additionally specialized equipment are available for laser microdissection, cell picking, micropatterning and micromanufacturing. Moreover high-end commertial software and freeware are available for  image processing and analysis.

The Live Cell Imaging facility is grateful to the founding agencies that allow its development: Vetenskapsrådet, the Knut and Alice Wallenberg Foundation, the Centre for Biosciences/CIMED and the KI fund for core facilities.

Today the LCI facility is proud of being part of the The Jonasson Centre for Medical Imaging, the network of Nikon Center of Excellence, as well as being member of several international imaging networks (Swedish Bioimaging Network, European Light Microscopy Initiative).

Jonasson Centre for Medical Imaging and Nikon Center of Excellence

The Jonasson Centre for Medical Imaging was established in 2014 after a donation from Kirstin och Rune Jonasson to the Royal Institute of Technology (KTH) in Sweden. The overall aim of the Centre is for healthcare, technology and research in the region to become world-leading within image function.

The LCI facility is happy to host 2 high-end microscopes that are part of the Jonasson Centre: The Pegasus and Orion systems allow researchers to perform confocal imaging and super-resolution microscopy.

In 2014, the LCI facility was awarded the prestigious Nikon Center of Excellence label. The facility became one of the few Centers of Excellence in the world. The Center officialises a tripartite collaboration between the Live Cell Imaging facility at the Karolinska Institute, the Royal School of Technology (KTH) and Nikon Instruments Europe.

This collaboration includes sharing equipment, resources and knowledge in order to offer the best possible service to the users of the LCI facility and contribute to the development of the Nikon confocals and software. The Nikon Center of Excellence comprises the 6 state-of-the-art Nikon systems present at the LCI facility.

Funding

Thanks to those supporting the LCI facility: the Knut and Alice Wallenberg Foundation, the Swedish Research Council, the Centre for Innovative Medicine and the Jonasson center at the Royal Institute of Technology,  Sweden.

Testimonials

- "The training was very good. It was the first time in my career I had such a personalised and complete training on microscopy. It is very useful to spend several hours with someone from the LCI staff during the training and learn how to image our samples in the best way possible."

- "The theory component of the training is invaluable."

- "Best imaging place ever!"

- "It's great that those extensive trainings exist! It really helps to get most out of your samples."

- "The staff of the facility are available to help troubleshoot at short notice and they are constantly bringing new technologies and techniques to the facility to improve results."

- "The service offered by the facility is outstanding."

- "The LCI imaging facility is the best imaging facility I encountered during my career regarding equipment (keeping up with new developments), training and support."