Widefield Microscopes at BIC
The BIC facility has several different widefield microscopes in open access for researchers.
Widefield Systems with Additional Applications
Basic Widefield Systems
- Leica DMRE upright
The Leica DMRE is an older upright manual fluorescence microscope for slide imaging.
Illumination: Halogen lamp for transmitted light and EBQ 100W Mercury Arc lamp for fluorescence.
Channels: DAPI, FITC, B/G/R triple band, R&B Phycoerythrin, Cy3.5, Cy5, Cy5.5
Objectives: 10X, 16X (immersion), 20X, 25X(oil), 40X(oil), 63X (oil), 100X (oil)
Camera: old Hamamatsu water-cooled CCD C4880 with 512x512 pixels of 24um size.
- Olympus IX73
The Olympus IX73 is an inverted microscope with the capacity for both colors- or monochromatic acquisitions of samples in dishes or slides (up to 3 slides simultaneously). It has an automated stage for tiles & position imaging and can acquire brightfield, phase contrast, and fluorescence images.
Illumination: Halogen lamp for transmitted light and CoolLED pe-300 white LED lamp (with UV=365nm, B=460nm and GYR=525-660nm).
Channels: Brightfield, Phase contrast, DAPI, FITC, TRITC, Cy3 and Cy5
Objectives (all dry/air – no immersion!): 2X, 4X, 10X, 20X and 40x
Camera: Olympus XC-10 IR color CCD. 14bit w 1376x1032 pixels of 6,45um size.
- Zeiss Calcium upright
The “Calcium upright” microscope is an upright manual fluorescence microscope equipped for imaging of very rapid fluorescence events, such as imaging of calcium fluctuations. Suitable container is a 35mm petri dish.
Illumination: Halogen lamp for transmitted light and Xenon Arc lamp for fluorescence.
Channels: Fura-2 (blue), Fura/GFP (blue/green), Fura/Rhod (blue/red), GFP/Rhod (green/red) and Filterset 46 (green)
Objectives: 20X and 63X (both water dipping) are mounted on the slide (however can be exchanged with other dipping objectives).
If you want more details about the different microscopes we have compiled a sheet for you under More information. The information may be useful when choosing between different microscopes.
You are always welcome to contact us if you need further information or guidance among the microscopes.
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Enjoy a collection of images made with microscopes within our facility!