High Content Systems at BIC
If you have live cells and need automated imaging for your research you are welcome to become one of our users. As a user you have access to our six different systems within this category of imaging techniques.
ZEISS Axio Scan.Z1 Digital Slide Scanner
The Axio Scan Z1 is a high throughput automated slide scanner which can process up to 100 slides in a single imaging session. This instrument is capable of multichromatic fluorescence imaging as well as brightfield imaging with colourmetric detection using high resolution air objectives.
- Illumination: Calibri 7 LED (385, 430, 475, 555, 590, 630 and 735nm)
- Emission filter: Emission filter 425/30 + 514/31 + 592/25 + 681/45 + 785/38
- Objectives: Fluar 5X/0.25, Plan Apochromat 10X/0.45, Plan Apochromat 20X/0.8, Plan Apochromat 40X/0.95
- Camera: Flour camera: Hamamatsu Orca Flash 4.0 V3 16-bit imaging w sCMOS, Color camera: Hitachi HV-F203
- Software: Zen Blue v3.1.
Satorius IncuCyte S3
Long-term imaging inside a cell culture incubator, multiwell plates, transmitted light, GFP/RFP fluorescence.
Mol. Devices ImageXpress Pico
The ImageXpress Pico cellular imaging system from MolecularDevices is a fully-integrated hardware and software system for automated acquisition and cellular analysis of images for high content imaging. This system allows imaging of samples on slides and in multi-well plates of various sizes (incl. 96, 384 wells) with different fluorescent channels (DAPI, FITC, CY3, CY5) at different magnifications (4x, 10x, 20x, 40x). The ImageXpress Pico is also equipped for transmitted light/colorimetric imaging. Images can be automatically analyzed for many different parameters using pre-made application modules (multi-wavelength cell scoring, count nuclei, live/dead, translocation, mitotic index, cell cycle, granularity, neurite outgrowth etc) and customized protocols. The system has a temperature controlled environment, allowing time-lapse imaging of living cells in CO2-independent medium.
- Software: Web-browser based CellReporterXpress, can be accessed within KI staff / eduroam WIFI connection.
The Leica DMI6000 widefield fluorescence system allows fluorescence and transmitted-light imaging of slides and multi-well plates automatically. It is equipped with 5x, 10x, 20x extra-long working distance objectives so that even cell culture multi-well plates can be used. The system has 2 fluorescence illumination sources: a LED unit with 365nm (DAPI/Hoechst, 488nm (GFP) and 530nm (CY3, RFP) LED lamps, and a fluorescence lamp and filter sets for DAPI/Hoechst, CFP, GFP, YFP, RFP and CY5/Alexa647.
The system is equipped with a climate chamber for live cell imaging for a few hours to multiple days. The acquisition software is LAS X (Leica) with a very basic user interface, Navigator stage overview, or and advanced Matri screening interface.
Zeiss Cell Observer
The Zeiss Cell Observer is a widefield microscope equipped with temperature, humidity and CO2 control for live cell imaging. This system facilitates bright field, epifluorescence, DIC and phase contrast microscopy.
- Illumination: Halogen lamp, Calibri 5 LED (385, 475, 555 and 630nm)
- Filters: DAPI: G365_FT395_BP445/50 (49), CFP: BP436/20_FT455_BP480/40 art.nr 1196-682 (47), HE DsRed: BP550/25_FT570_BP605/70 (43), Cy5: BP640/30, FT660, BP690/50 (50) and YFP: BP500/20_FT515_BP535/30 art.nr. 1196-681 (in storage) (46)
- Objectives: EC Plan-NEOFLUAR 10X/0.3, LD Plan-NEOFLUAR 20X/0.4, LD Plan-NEOFLUAR 40X/0.6, EC Plan-NEUFLUAR 10X/0.3 Ph1, LD Plan-NEOFLUAR 20X/0.4 Ph2, LD Plan-NEOFLUAR 40X/0.6 Ph2
- Cameras: Zeiss AxioCam MRm cooled 12-bit 1388x1040 pixels
- Motorized stage: accommodates all multi-well plates, slides, petri dishes of 100mm or smaller
Choosing a microscope
If you are interested in more details we have compiled a sheet for you under More information. The information could be useful when choosing between different microscopes.
You are always welcome to contact us if you need further information or guidance among the microscopes.
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Enjoy a collection of images made with microscopes within our facility!