NK cell related publications

2025

Enhancing human NK cell antitumor function by knocking out SMAD4 to counteract TGFβ and activin A suppression.
Rea A, Santana-Hernández S, Villanueva J, Sanvicente-García M, Cabo M, Suarez-Olmos J, Quimis F, Qin M, Llorens E, Blasco-Benito S, Torralba-Raga L, Perez L, Bhattarai B, Alari-Pahissa E, Georgoudaki AM, Balaguer F, Juan M, Pardo J, Celià-Terrassa T, Rovira A, Möker N, Zhang C, Colonna M, Spanholtz J, Malmberg KJ, Montagut C, Albanell J, Güell M, López-Botet M, Muntasell A
Nat Immunol 2025 Apr;26(4):582-594

CD73 promotes the maturation of murine NK cells and their survival in the tumor microenvironment.
Parra-Tello B, García-Gómez M, Rekus-Polanska E, Malgue F, Charlín S, Hernández-Oliveras A, Reyes-Alvarez J, Rosemblatt M, Lundqvist A, Lladser A, Bono MR, Sauma D
J Leukoc Biol 2025 Feb;():

The HLA-B -21 M/T dimorphism associates with disease severity in COVID-19.
Strunz B, Momayyezi P, Bilev E, Muvva JR, Chen P, Bister J, Schaffer M, Akber M, Cornillet M, , Horowitz A, Malmberg KJ, Rooyackers O, Aleman S, Ljunggren HG, Björkström NK, Strålin K, Hammer Q
Genes Immun 2025 Feb;26(1):70-74

Targeting HLA-E-overexpressing cancers with a NKG2A/C switch receptor.
Sætersmoen M, Kotchetkov IS, Torralba-Raga L, Mansilla-Soto J, Sohlberg E, Krokeide SZ, Hammer Q, Sadelain M, Malmberg KJ
Med 2025 Feb;6(2):100521

Interleukin-12 decorated nanosized semiflexible Immunofilaments enable directed targeting and augmented IFNγ responses of natural killer cells.
Weiss L, Schluck M, Classens R, de Jonge PKJD, van der Waart A, Nguyen KG, Nguyen TT, Zaharoff DA, Malmberg KJ, Dolstra H, Figdor CG, Sohlberg E, Hammink R
Acta Biomater 2025 Jan;191():386-397

A thermoplastic chip for 2D and 3D correlative assays combining screening and high-resolution imaging of immune cell responses.
van Ooijen H, Verron Q, Zhang H, Sandoz PA, Frisk TW, Carannante V, Olofsson K, Wagner AK, Sandström N, Önfelt B
Cell Rep Methods 2025 Jan;5(1):100965

Evaluation of lymphocyte infiltration into cancer spheroids by immunofluorescent staining and 3D imaging.
Cruz De Los Santos M, Lundqvist A
Methods Cell Biol 2025 ;191():269-287

2024

Cellular and secretome profiling uncover immunological biomarkers in the prognosis of renal cell carcinoma patients.
Tong L, Kremer V, Neo SY, Seitz C, Tobin NP, Seliger B, Harmenberg U, Colón E, Scherman Plogell AH, Liu LL, Lundqvist A
Oncoimmunology 2025 Dec;14(1):2481109

Emerging mutation in SARS-CoV-2 facilitates escape from NK cell recognition and associates with enhanced viral fitness.
Bilev E, Wild N, Momayyezi P, Sala BM, Sun R, Sandalova T, Marquardt N, Ljunggren HG, Achour A, Hammer Q
PLoS Pathog 2024 Dec;20(12):e1012755

Natural killer cell biology and therapy in multiple myeloma: challenges and opportunities.
Bisht K, Merino A, Igarashi R, Gauthier L, Chiron M, Desjonqueres A, Smith E, Briercheck E, Romee R, Alici E, Vivier E, O'Dwyer M, van de Velde H
Exp Hematol Oncol 2024 Nov;13(1):114

The G Protein-Coupled Receptor GPR56 Is an Inhibitory Checkpoint for NK Cell Migration.
Palacios D, Majhi RK, Szabo EK, Clement D, Lachota M, Netskar H, Penna L, Krokeide SZ, Vincenti M, Kveberg L, Malmberg KJ
J Immunol 2024 Nov;213(9):1349-1357

Genetic ablation of adhesion ligands mitigates rejection of allogeneic cellular immunotherapies.
Hammer Q, Perica K, Mbofung RM, van Ooijen H, Martin KE, Momayyezi P, Varady E, Pan Y, Jelcic M, Groff B, Abujarour R, Krokeide SZ, Lee T, Williams A, Goodridge JP, Valamehr B, Önfelt B, Sadelain M, Malmberg KJ
Cell Stem Cell 2024 Sep;31(9):1376-1386.e8

High-dimensional single-cell analysis of human natural killer cell heterogeneity.
Rebuffet L, Melsen JE, Escalière B, Basurto-Lozada D, Bhandoola A, Björkström NK, Bryceson YT, Castriconi R, Cichocki F, Colonna M, Davis DM, Diefenbach A, Ding Y, Haniffa M, Horowitz A, Lanier LL, Malmberg KJ, Miller JS, Moretta L, Narni-Mancinelli E, O'Neill LAJ, Romagnani C, Ryan DG, Sivori S, Sun D, Vagne C, Vivier E
Nat Immunol 2024 Aug;25(8):1474-1488

Pan-cancer profiling of tumor-infiltrating natural killer cells through transcriptional reference mapping.
Netskar H, Pfefferle A, Goodridge JP, Sohlberg E, Dufva O, Teichmann SA, Brownlie D, Michaëlsson J, Marquardt N, Clancy T, Horowitz A, Malmberg KJ
Nat Immunol 2024 Aug;25(8):1445-1459

Efficacy of T-cell assays for the diagnosis of primary defects in cytotoxic lymphocyte exocytosis.
Chiang SCC, Covill LE, Tesi B, Campbell TM, Schlums H, Nejati-Zendegani J, Mördrup K, Wood S, Theorell J, Sekine T, Al-Herz W, Akar HH, Belen FB, Chan MY, Devecioglu O, Aksu T, Ifversen M, Malinowska I, Sabel M, Unal E, Unal S, Introne WJ, Krzewski K, Gilmour KC, Ehl S, Ljunggren HG, Nordenskjöld M, Horne A, Henter JI, Meeths M, Bryceson YT
Blood 2024 Aug;144(8):873-887

Egr2 to the rescue: nanoparticles revitalize natural killer cells in the fight against cancer.
Pfefferle A, Phuyal S, Netskar H, Malmberg KJ
EMBO J 2024 Jul;43(13):2527-2529

A temporal developmental map separates human NK cells from noncytotoxic ILCs through clonal and single-cell analysis.
Vo DN, Yuan O, Kanaya M, Telliam-Dushime G, Li H, Kotova O, Caglar E, Honnens de Lichtenberg K, Rahman SH, Soneji S, Scheding S, Bryder D, Malmberg KJ, Sitnicka E
Blood Adv 2024 Jun;8(11):2933-2951

Tumor-associated NK cells drive MDSC-mediated tumor immune tolerance through the IL-6/STAT3 axis.
Neo SY, Tong L, Chong J, Liu Y, Jing X, Oliveira MMS, Chen Y, Chen Z, Lee K, Burduli N, Chen X, Gao J, Ma R, Lim JP, Huo J, Xu S, Alici E, Wickström SL, Haglund F, Hartman J, Wagner AK, Cao Y, Kiessling R, Lam KP, Westerberg LS, Lundqvist A
Sci Transl Med 2024 May;16(747):eadi2952

Redirecting NK cells to the lymph nodes to augment their lymphoma-targeting capacity.
Sanz-Ortega L, Leijonhufvud C, Schoutens L, Lambert M, Levy E, Andersson A, Wahlin BE, Carlsten M
NPJ Precis Oncol 2024 May;8(1):108

Engineering immune-evasive allogeneic cellular immunotherapies.
Martin KE, Hammer Q, Perica K, Sadelain M, Malmberg KJ
Nat Rev Immunol 2024 Apr;():

KIR2DS1 and KIR2DL1-C245 Dominantly Repress NK Cell Degranulation Triggered by Monoclonal or Bispecific Antibodies, whereas Education by Uptuning Inhibitory Killer Ig-related Receptors Exerts No Advantage in Ab-dependent Cellular Cytotoxicity.
Leijonhufvud C, Sanz-Ortega L, Schlums H, Gaballa A, Andersson A, Eriksson C, Segerberg F, Uhlin M, Bryceson YT, Carlsten M
J Immunol 2024 Mar;212(5):868-880

Harnessing upregulated E-selectin while enhancing SDF-1α sensing redirects infused NK cells to the AML-perturbed bone marrow.
Sanz-Ortega L, Andersson A, Carlsten M
Leukemia 2024 Mar;38(3):579-589

Natural killer cells drive 4-1BBL positive uveal melanoma towards EMT and metastatic disease.
Neo SY, Oliveira MMS, Tong L, Chen Y, Chen Z, Cismas S, Burduli N, Malmerfelt A, Teo JKH, Lam KP, Alici E, Girnita L, Wagner AK, Westerberg LS, Lundqvist A
J Exp Clin Cancer Res 2024 Jan;43(1):13

2023

Viral escape from NK-cell-mediated immunosurveillance: A lesson for cancer immunotherapy?
Momayyezi P, Bilev E, Ljunggren HG, Hammer Q
Eur J Immunol 2023 Nov;53(11):e2350465

Accumulation of tissue-resident natural killer cells, innate lymphoid cells, and CD8+ T cells towards the center of human lung tumors.
Brownlie D, von Kries A, Valenzano G, Wild N, Yilmaz E, Säfholm J, Al-Ameri M, Alici E, Ljunggren HG, Schliemann I, Aricak O, Haglund de Flon F, Michaëlsson J, Marquardt N
Oncoimmunology 2023 ;12(1):2233402

Challenges in αCD38-chimeric antigen receptor (CAR)-expressing natural killer (NK) cell-based immunotherapy in multiple myeloma: Harnessing the CD38dim phenotype of cytokine-stimulated NK cells as a strategy to prevent fratricide.
Karvouni M, Vidal-Manrique M, Susek KH, Hussain A, Gilljam M, Zhang Y, Gray JD, Lund J, Kaufmann G, Ljunggren HG, Ji H, Lundqvist A, Wagner AK, Guo W, Alici E
Cytotherapy 2023 Jul;25(7):763-772

A tractable microscopy- and flow cytometry-based method to measure natural killer cell-mediated killing and infiltration of tumor spheroids.
Schwietzer YA, Susek KH, Chen Z, Alici E, Wagner AK
Methods Cell Biol 2023 ;178():43-61

Renal cell carcinoma escapes NK cell-mediated immune surveillance through the downregulation of DNAM-1.
Tong L, Kremer V, Neo SY, Liu Y, Chen Y, Wagner AK, Yang Y, Chen Z, Seitz C, Tobin NP, Ligtenberg MA, Alici E, Chen X, Haglund F, Seliger B, Harmenberg U, Colón E, Plogell AS, Liu LL, Lundqvist A
Cancer Commun (Lond) 2023 Jul;43(7):855-859

SHP-1 localization to the activating immune synapse promotes NK cell tolerance in MHC class I deficiency.
Schmied L, Luu TT, Søndergaard JN, Hald SH, Meinke S, Mohammad DK, Singh SB, Mayer C, Perinetti Casoni G, Chrobok M, Schlums H, Rota G, Truong HM, Westerberg LS, Guarda G, Alici E, Wagner AK, Kadri N, Bryceson YT, Saeed MB, Höglund P
Sci Signal 2023 Apr;16(780):eabq0752

MetaGate: Interactive Analysis of High-Dimensional Cytometry Data with Meta Data Integration.
Ask EH, Tschan-Plessl A, Hoel HJ, Kolstad A, Holte H, Malmberg KJ
bioRxiv 2023 Nov;():

Genetic ablation of adhesion ligands averts rejection of allogeneic immune cells.
Hammer Q, Perica K, van Ooijen H, Mbofung R, Momayyezi P, Varady E, Martin KE, Pan Y, Jelcic M, Groff B, Abujarour R, Krokeide S, Lee T, Williams A, Goodridge JP, Valamehr B, Önfelt B, Sadelain M, Malmberg KJ
bioRxiv 2023 Oct;():

Mapping the chemotactic landscape in NK cells reveals subset-specific synergistic migratory responses to dual chemokine receptor ligation.
Lachota M, Zielniok K, Palacios D, Kanaya M, Peena L, Hoel HJ, Wiiger MT, Kveberg L, Hautz W, Zagożdżon R, Malmberg KJ
EBioMedicine 2023 Oct;96():104811

The Lysosomal Calcium Channel TRPML1 Maintains Mitochondrial Fitness in NK Cells through Interorganelle Cross-Talk.
Clement D, Szabo EK, Krokeide SZ, Wiiger MT, Vincenti M, Palacios D, Chang YT, Grimm C, Patel S, Stenmark H, Brech A, Majhi RK, Malmberg KJ
J Immunol 2023 Nov;211(9):1348-1358

Affibody-based hBCMA x CD16 dual engagers for NK cell-mediated killing of multiple myeloma cells.
Giang KA, Boxaspen T, Diao Y, Nilvebrant J, Kosugi-Kanaya M, Kanaya M, Krokeide SZ, Lehmann F, Svensson Gelius S, Malmberg KJ, Nygren PÅ
N Biotechnol 2023 Nov;77():139-148

Allelic variation of KIR and HLA tunes the cytolytic payload and determines functional hierarchy of NK cell repertoires.
Philippon C, Tao S, Clement D, Haroun-Izquierdo A, Kichula KM, Netskar H, Brandt L, Oei VS, Kanaya M, Lanuza PM, Schaffer M, Goodridge JP, Horowitz A, Zhu F, Hammer Q, Sohlberg E, Majhi RK, Kveberg L, Önfelt B, Norman PJ, Malmberg KJ
Blood Adv 2023 Aug;7(16):4492-4504

Effect of mTOR Inhibition with Sirolimus on Natural Killer Cell Reconstitution in Allogeneic Stem Cell Transplantation.
Haroun-Izquierdo A, Lanuza PM, Pfefferle A, Netskar H, Ask EH, Törlén J, Björklund A, Sohlberg E, Malmberg KJ
Transplant Cell Ther 2023 Jun;29(6):376.e1-376.e11

CD25bright NK cells display superior function and metabolic activity under regulatory T cell-mediated suppression.
Chen Z, Tong L, Neo SY, Li S, Gao J, Schlisio S, Lundqvist A
Oncoimmunology 2023 ;12(1):2175517

Generation of tumor spheroids in microwells to study NK cell cytotoxicity, infiltration and phenotype.
Carannante V, Sandström N, Olofsson K, Van Ooijen H, Hell B, Wiklund M, Önfelt B
Methods Cell Biol 2023 ;178():195-208

In vitro models to study natural killer cell dynamics in the tumor microenvironment.
Carannante V, Wiklund M, Önfelt B
Front Immunol 2023 ;14():1135148

Improved Leukemia Clearance After Adoptive Transfer of NK Cells Expressing the Bone Marrow Homing Receptor CXCR4R334X.
Segerberg F, Lambert M, Sanz-Ortega L, Andersson A, Childs RW, Carlsten M
Hemasphere 2023 Nov;7(11):e974

Perturbed epigenetic transcriptional regulation in AML with IDH mutations causes increased susceptibility to NK cells.
Palau A, Segerberg F, Lidschreiber M, Lidschreiber K, Naughton AJ, Needhamsen M, Jung LA, Jagodic M, Cramer P, Lehmann S, Carlsten M, Lennartsson A
Leukemia 2023 Sep;37(9):1830-1841

Loss of metabolic fitness drives tumor resistance after CAR-NK cell therapy and can be overcome by cytokine engineering.
Li L, Mohanty V, Dou J, Huang Y, Banerjee PP, Miao Q, Lohr JG, Vijaykumar T, Frede J, Knoechel B, Muniz-Feliciano L, Laskowski TJ, Liang S, Moyes JS, Nandivada V, Basar R, Kaplan M, Daher M, Liu E, Li Y, Acharya S, Lin P, Shanley M, Rafei H, Marin D, Mielke S, Champlin RE, Shpall EJ, Chen K, Rezvani K
Sci Adv 2023 Jul;9(30):eadd6997

2022

Quadruple gene-engineered natural killer cells enable multi-antigen targeting for durable antitumor activity against multiple myeloma.
Cichocki F, Bjordahl R, Goodridge JP, Mahmood S, Gaidarova S, Abujarour R, Davis ZB, Merino A, Tuininga K, Wang H, Kumar A, Groff B, Witty A, Bonello G, Huffman J, Dailey T, Lee TT, Malmberg KJ, Walcheck B, Höpken U, Rehm A, Valamehr B, Miller JS
Nat Commun 2022 Nov;13(1):7341

Adaptive single-KIR+NKG2C+ NK cells expanded from select superdonors show potent missing-self reactivity and efficiently control HLA-mismatched acute myeloid leukemia.
Haroun-Izquierdo A, Vincenti M, Netskar H, van Ooijen H, Zhang B, Bendzick L, Kanaya M, Momayyezi P, Li S, Wiiger MT, Hoel HJ, Krokeide SZ, Kremer V, Tjonnfjord G, Berggren S, Wikström K, Blomberg P, Alici E, Felices M, Önfelt B, Höglund P, Valamehr B, Ljunggren HG, Björklund A, Hammer Q, Kveberg L, Cichocki F, Miller JS, Malmberg KJ, Sohlberg E
J Immunother Cancer 2022 Nov;10(11):

Generation of NK cells with chimeric-switch receptors to overcome PD1-mediated inhibition in cancer immunotherapy.
Susek KH, Schwietzer YA, Karvouni M, Gilljam M, Keszei M, Hussain A, Lund J, Kashif M, Lundqvist A, Ljunggren HG, Nahi H, Wagner AK, Alici E
Cancer Immunol Immunother 2022 Nov;():

A tractable microscopy- and flow cytometry-based method to measure natural killer cell-mediated killing and infiltration of tumor spheroids
Schwietzer Y A, Susek K H, Chen Z, Alici E, Wagner A K
Methods in Cell Biology, 2022

Miniaturized and multiplexed high-content screening of drug and immune sensitivity in a multichambered microwell chip.
Sandström N, Carannante V, Olofsson K, Sandoz PA, Moussaud-Lamodière EL, Seashore-Ludlow B, Van Ooijen H, Verron Q, Frisk T, Takai M, Wiklund M, Östling P, Önfelt B
Cell Rep Methods 2022 Jul;2(7):100256

Autologous NK cells as consolidation therapy following stem cell transplantation in multiple myeloma.
Nahi H, Chrobok M, Meinke S, Gran C, Marquardt N, Afram G, Sutlu T, Gilljam M, Stellan B, Wagner AK, Blomberg P, Holmqvist PH, Walther-Jallow L, Mellström K, Liwing J, Gustafsson C, Månsson R, Klimkowska M, Gahrton G, Lund J, Ljungman P, Ljunggren HG, Alici E
Cell Rep Med 2022 Feb;3(2):100508

2021

LIR-1 educates expanded human NK cells and defines a unique antitumor NK cell subset with potent antibody-dependent cellular cytotoxicity.
Leijonhufvud C, Reger R, Segerberg F, Theorell J, Schlums H, Bryceson YT, Childs RW, Carlsten M
Clin Transl Immunology 2021 ;10(10):e1346

NK cells integrate signals over large areas when building immune synapses but require local stimuli for degranulation.
Verron Q, Forslund E, Brandt L, Leino M, Frisk TW, Olofsson PE, Önfelt B
Sci Signal 2021 05;14(684):

The transcription factor Bcl11b promotes both canonical and adaptive NK cell differentiation.
Holmes TD, Pandey RV, Helm EY, Schlums H, Han H, Campbell TM, Drashansky TT, Chiang S, Wu CY, Tao C, Shoukier M, Tolosa E, Von Hardenberg S, Sun M, Klemann C, Marsh RA, Lau CM, Lin Y, Sun JC, Månsson R, Cichocki F, Avram D, Bryceson YT
Sci Immunol 2021 03;6(57):

2020

Thioredoxin activity confers resistance against oxidative stress in tumor-infiltrating NK cells.
Yang Y, Neo SY, Chen Z, Cui W, Chen Y, Guo M, Wang Y, Xu H, Kurzay A, Alici E, Holmgren L, Haglund F, Wang K, Lundqvist A
J Clin Invest 2020 10;130(10):5508-5522