PSF NEWS

PPS at the Integrated Structural Biology symposia, SciLifeLab

2023-12-06

The Integrated Structural Biology platform at SciLifeLab is linked to a network of important stakeholders, offering expertise in related areas. For the second time at the ISB annual user symposia, Tomas Nyman presented the national infrastructure Protein Production Sweden.

PPS at the Integrated Structural Biology symposia

2022-12-12

The Integrated Structural Biology platform at SciLifeLab is linked to a network of important stakeholders, offering expertise in related areas. At the ISB symposia held at SciLifeLab, Tomas Nyman presented the national infrastructure Protein Production Sweden.

PSF teaches at the InfraLife Integrative Structural Biology course

2022-08-25

In the last week of August, 26 PhD students visited the PSF labs in Biomedicum to get hands-on experience in isolating and freezing protein crystals, trying out xray model-building and refinement software, getting a close look at a hi-throughput protein production lab and learn about different E.coli strains and different expression and purification techniques. This practical and hands-on visit was very much appreciated by the students. We hope to participate in the course next time.

The InfraLife is an initiative to maximize benefits of Sweden’s large-scale research infrastructures; ESS, MAX IV and SciLifeLab. It is a four-year project funded by the Swedish research council (VR) grant “For accessibility to infrastructure” that started in 2021. To arrange courses was part of the original application, to increase knowledge about available techniques at the different infrastructures, and how they complement one another. The first suggested course theme was structural biology, hence the project arranged the InfraLife integrative structural biology  course in the fall of 2022.

KI Core Facility Poster Lunch

2022-08-11

About 20 Core facilities from KI Nord och KI Solna arranged a joint lunch poster session in the Biomedicum atrium. The event were advertized primariliy towards PhD students and post-docs. Lunch sandwishes and drinks were offered. Quite many young researchers showed up and we had many interesting discussions. This was a good format for CF-reach out and we plan to arrange similar events twice per year, alternating between north campus (Solna) and south campus (Flemingsberg).

PSF presents at the Sweprot conference in Tällberg

2022-06-17

PSF presented two posters and had many fruitful interactions at the yearly conference on Macromolecular Structure and Function in Tällberg, Dalarna.

Eukaryotic protein expression in collaboration with SciLifeLab Solna

2022-02-12

PSF is now ready to offer protein expression services in eukaryotic systems, predominantly HEK293 cells. The quick start is made possible through a colloboration aggreement with SciLifeLab Solna, who make their modern BSL2-lab available to PSF and PSF projects. As part of the agreement PSF takes over the responsibility of managing the maintenance of the cell lab, introducing new users and give support. The PSF group member Juni Andréll will be the responsible lab-manager. Contact PSF for questions at psf@mbb.ki.se

PSF offers eukaryotic protein production

2022-02-12

Starting this spring, PSF are happy to offer protein expression services in eukaryotic systems, predominantly HEK293 cells. Contact PSF for questions at psf@mbb.ki.se

PSF welcomes new group member Juni Andréll

2022-01-31

We are very happy to welcome Juni Andréll to the group. Juni will be heading the work in eukaryotic protein production, predominantly in HEK293 and CHO cells.

PSF receives 750,000 SEK support from KI for pilot project at Testa Center

2021-11-26

After finalizing the procurement agreement between KI and Testa Center, the idea is that PSF will act as a supporting link for researchers who aim to utilize the agreement and perform work at Testa Center. For this, PSF staff need to build an understanding of the industrial scale technologies used at Testa C. The KI vice chancellor has approved to support the costs for educational pilot work. The project is planned for the spring of 2022.

New national infrastructure PPS awarded 42 MSEK by VR

2021-10-01

Together with six other protein production facilities (at UmU, KTH, GU and LU), PSF now forms the national infrastructure Protein Production Sweden (PPS). The funding runs 2022-2026 and total funding is 42 MSEK, with equal contribution from the universities. PSF receives 1.3 MSEK/year plus equal contribution from KI. This infrastructure offers recombinant protein production in a variety of expressinon systems: E.coli, insect cells, yeast, mammalian cells, plant cells, and cell-free expression. The organisation, website and user request web portal is under construction, but all sites are already operational and continuously take requests.

PSF 10 year anniversary!

2021-09-12

The PSF officially launched on September 12, 2011. Then in the old MBB dept quarters in Scheele laboratories, the core facility formed as a spin-off from the Structural Genomics Consortium (SGC), Stockholm branch. When the 6-year old SGC-Sthlm project run out of funds, the instrumentation, protocols, and knowhow was transformed into a core facility for protein production, macromolecular crystallography, and biophysical characterization of proteins.

From 2012 to 2015 the number of PI-groups working with PSF steadily increased, and has since stayed stable at 60-70 unique PI-groups each year.

Frame agreement between KI and Testa Center in place

2021-09-06

Karolinska Institutet (KI) recently signed a frame agreement with Testa Center, Uppsala, to enable access to laboratories for the scale up of biological products from lab bench to industrial-scale quantities. The frame agreement was worked in place by Tomas Nyman, Richard Cowburn and Hannes Olsson, on request from the vice chancellor.