3D-EM - our offer
The 3D-EM Core Facility has high-end electron microscopes and sample preparation equipment for biological samples. The facility is primarily training-based, in that we will train you to operate the microscopes and instruments so you can collect your own data.
For data collection by staff, an additional 650 SEK/hr fee is charged on top of instrument fees. All our available instruments are listed below, as well as information regarding pricing and training.
Main areas/services
- Single particle cryo EM
- Negative stain EM
- Tomography
- Volume Imaging
- Lamella milling
- FIB milling with liftout
- Cryo-CLEM
Available instruments
Electron microscopes
- 120 kV LaB6 microscope: Talos 120C G2 with Ceta-D detector
- 300 kV cold-FEG microscope: Krios G3i with Gatan K3 detector and Ceta-D detector.
- Aquilos 2 cryo-FIB-SEM
- Leica SP8 cryo-confocal microscope
Sample preparation
- Vitrobot Mk 1
- Vitrobot Mk 4
- Leica EM ICE high pressure freezer
- Glow discharger
- Plasma cleaner
- Leica ACE600 sputter coater
Access and booking
Only registered users can have an access to the facility. Registration is to be completed on the 3D-EM Core Facility iLab site and must be approved by PI of the user. The PI provides each user with project number to facilitate monthly payment via iLab.
Teaching and training
We will arrange courses and workshops periodically. However, users are encouraged to receive individual TEM training followed by a test on the 120 kV Talos microscope following which, we will grant them card access to the facility. Once you have passed the test on the Talos and have access to the facility, you can be trained on other instruments as needed for your project.
Contact us at 3d-em@cmb.ki.se for more details.
Price for service
| Internal | External* | Companies* | |
|---|---|---|---|
| Aquilos 2 RT 3h | 1 260 | 1 260 | 6 000 |
| Aquilos 2 RT/cryo 24h | 3 000 | 3 000 | 18 000 |
| Krios 4h | 2 400 | 2400 | 24 000 |
| Krios 24h | 6 000 | 6 000 | 60 000 |
| Talos 120C 3h | 720 | 720 | 7 200 |
| SP8 cryo-confocal 24h | 960 | 960 | 9 600 |
| Vitrobot 2h | 240 | 240 | 1 200 |
| Leica EM ICE 6h | 720 | 720 | 3 600 |
All prices include KI overhead. * External/company usage of the equipment requires facility manager/director support for setup/startup that is billed at 650 SEK per hour. Training time is free for KI personnel (plus standard instrument hourly costs) but charged at an additional 650 SEK per hour for external/companies. International academic customers pay double hourly microscope fees, all other costs same.