Molecular Pharmacology of GPCRs – Pawel Kozielewicz's team

We are interested in understanding the role of understudied G protein-coupled receptors - GPCRs - in health and disease. We are employing biophysical and computational methods to understand pharmacology, signaling and disease relevance of orphan GPCRs to target them in therapy.

Part of the:

We are interested in understanding the role of GPCRs in health and disease, with a focus on cancer and obesity. Of particular interest to us are understudied orphan GPCRs. These receptors bear untapped therapeutic potential and a lof of them are highly expressed or mutated in these diseases. To shed light on the functions of orphan GPCRs in these conditions, we aim to employ an integrative approach. We will study these proteins in different systems, ranging from HEK293 cells to patients' samples, and investigate phenomena at different levels: from receptor conformational changes to impact on cell phenotype.

Our methodologies include but are not limited to:

  • Bioluminescence resonance energy transfer (BRET)
  • CRISPR-Cas9 genome editing
  • Molecular modelling with ligand docking and molecular dynamics simulations
  • Proximal labelling techniques
  • Analysis of large genomic datasets

Projects:

  • Analysis of the role of orphan GPCRs in cancer
  • Investigations into orphan GPCRs as regulators of metabolism
  • Understandng the pharmacology of Smoothened

Publications

All publications from group members

Staff and contact

Group leader

All members of the group

Visiting address

Karolinska Institutet, Physiology and Pharmacology, Solnavägen 9, Biomedicum C5, Stockholm, Stockholms län, 17165, Sweden

Postal address

Karolinska Institutet, Physiology and Pharmacology, Tomtebodavägen 16, Biomedicum C5, Stockholm, Stockholms län, 17165, Sweden