The 60-year old cohort
An epidemiological cohort study of men and women, born 1937 and 1938 in Stockholm County.
The cohort of 60-year old men and women consists of every third man and woman, who during one year (1997 01 07 - 1998 06-30) reached the age of 60, and who subsequently was invited to a careful health control, including blood sampling, and completion of a questionnaire (response rate 78 %). Blood samples and DNA were stored in -80 oC freezers (4 freezers) both at Karolinska Hospital and the Karolinska Institutet. DNA has been extracted from all subjects and 4232 individuals participated at the health check-up. The predictive role of different risk exposures/biomarkers for cardiovascular diseases (CVD) is assessed through annual matching against available national registers. So far 408 incident CVD cases have been registered (up to 2010, on average 12 years of follow up). About 35 international original articles have been generated from the database (see a selection in the reference list).
Analyses of baseline data indicate that a variety of metabolic disturbances go unrecognized in this age group, many of which are attributed to low levels of physical exercise and bad life-style habits. Through analyses of incident CVD events using nested case-control designs two novel biomarkers have been identified; 1) Anti-PC (phosphorylcholine) a natural IgM antibody of which low levels reflect disturbed immunological defense mechanisms of the vascular wall and 2) CD 93 of which low levels are related to an increased risk for CVD. Novel potential pathways for CVD are currently investigated through determinations of miRNAs in a nested sample of incident myocardial infarctions and controls through collaboration with the University of Milan, Italy. The running of genome-wide analyses (GWAs) is planned. In addition, particular emphasis will be put on evaluating novel inflammatory biomarkers (immunological antibodies like Ox Cardiolipin, PCSK9 etc.) by multiplex techniques such as the Meso Scale platform.
Baseline data, whole cohort
- Waist-hip ratio
- Sagittal abdominal diameter
- Mean diastolic blood pressure
- Mean systolic blood pressure
- ECG (Minnesota coded including Lvh)
- Total cholesterol
- Uric acid
Nested case-control data
- Antibodies against Ox Cardiolipin
Link to questionnaire (Pdf file, 1 Mb)
The cohort is utilized for cohort analyses in four ongoing EU-studies:
- CVD-IMMUNE, for exploration of immunological mechanisms behind CVD
- IMPROVE, for exploration of mechanisms/factors behind atherosclerosis progression
- CEANS, exploration of air pollution and atherosclerosis and CVD risk
- SUMMIT for investigations of biomarkers related to CVD-risk in diabetics