3D-EM

Cellular electron microscopy has been a key technique for understanding the cell’s core elements and mechanisms since the 1950s. Electron microscopic methods have laid the foundation for a number of Nobel Prizes and have given us much of the basic understanding of human cells’ overall structure, physiology and mechanisms—basically the foundation of all modern medicine.

In recent years, electron microscopy has undergone extremely rapid development through a combination of significantly better optics, sample processing, detectors and image processing. The development has given rise to the so-called “Resolution Revolution,” which in recent years has gradually increased the average resolution of biological preparations significantly. Part of the discoveries and methodology resulted in a Nobel Prize to Richard Henderson, Jaques Dubochet and Joachim Frank. At the beginning of this revolution, a visionary decision of the then Karolinska Institutet’s (KI’s) leadership was made to build premises for a future electron microscopy center nearby the Biomedicum building at the KI Solna campus. These premises have now been built specifically for high-end biomedical electron microscopy. During 2019-2020 we will equip the newly built KI 3D-EM center with the best possible equipment and thereby enable a much broader group of KI researchers to utilize the enormous advances in electron microscopy in recent years.

Picture of microscope Krios G3i and the Talos 120C

Instruments available

Electron microscopes

Available for use:

120 kV LaB6microscope: Talos 120C G2 with Ceta-D detector

300 kV FEG microscope: Krios G3i with Gatan K3 detector and Ceta-D detector.
Aquilos 2 cryo-FIB-SEM

Leica SP8 cryo-confocal microscope

 

Sample preparation

Available:

  • Leica Microtome 1
  • Leica Microtome 2
  • Vitrobot Mk 1
  • Vitrobot Mk 4
  • Glow discharger
  • Plasma cleaner

Access and booking

Only registered users can have an access to the facility. Registration is to be done at 3D-EM Core Facility Service and must be approved by PI of the user. The PI provides each user with project number to facilitate the payment via iLab booking system.

Teaching and training

We will arrange courses and workshops. However, users are encouraged to receive personal training followed by a test that will open the access to the 120 kV Talos microscope. Please contact Sergej.Masich@ki.se for more details.

Prices for service

Prices for service and access for equipment currently available (including local overhead):

                                     Internal          External*       Companies*

Aquilos 2 RT 3h              1050              1050                 5000

Aquilos 2 RT/cryo 24h    2500              2500                15000

Krios 3h                          2000              2000                20000

Krios 24h                        5000              5000                50000

Talos 120C 3h                  600                600                  6000

SP8 cryo-confocal 24h     800                800                  8000

 

*external/company usage of the Aquilos2, Krios and SP8 cryo-confocal requires facility manager/director support for setup/startup and that is billed at 550 SEK per hour. Training time is free for KI personnel but charged at 550 SEK per hour for external/companies. International academic customers pay double hourly microscope fees, all other costs same.

Contact us

Martin Hällberg

Facility director

Sergej Masich

Facility manager

Amy Bondy

Facility manager

Publications resulting from 3D-EM facility use

Structural basis for late maturation steps of the human mitoribosomal large subunit
Cipullo M, Gese GV, Khawaja A, Hällberg BM*, Rorbach J*.
Nature Communications, June 16, 2021, doi: 10.1038/s41467-021-23617-8.

Plasmonic Coupling in Silver Nanoparticle Aggregates and Their Polymer Composite Films for Near-Infrared Photothermal Biofilm Eradication.
Merkl P, Zhou S, Zaganiaris A, Shahata M, Eleftheraki A, Thersleff T, Sotiriou GA
ACS Appl Nano Mater 2021 May;4(5):5330-5339

Antiviral Activity of Silver, Copper Oxide and Zinc Oxide Nanoparticle Coatings against SARS-CoV-2.
Merkl P, Long S, McInerney GM, Sotiriou GA
Nanomaterials (Basel) 2021 May;11(5):

Structure-guided multivalent nanobodies block SARS-CoV-2 infection and suppress mutational escape.
Koenig PA, Das H, Liu H, Kümmerer BM, Gohr FN, Jenster LM, Schiffelers LDJ, Tesfamariam YM, Uchima M, Wuerth JD, Gatterdam K, Ruetalo N, Christensen MH, Fandrey CI, Normann S, Tödtmann JMP, Pritzl S, Hanke L, Boos J, Yuan M, Zhu X, Schmid-Burgk JL, Kato H, Schindler M, Wilson IA, Geyer M, Ludwig KU, Hällberg BM, Wu NC, Schmidt FI
Science 2021 02;371(6530):

Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch.
Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep 2020 Dec;33(12):108529

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2.
Custódio TF, Das H, Sheward DJ, Hanke L, Pazicky S, Pieprzyk J, et al
Nat Commun 2020 11;11(1):5588

A latent lineage potential in resident neural stem cells enables spinal cord repair.
Llorens-Bobadilla E, Chell JM, Le Merre P, Wu Y, Zamboni M, Bergenstråhle J, et al
Science 2020 10;370(6512):

An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction.
Hanke L, Vidakovics Perez L, Sheward DJ, Das H, Schulte T, Moliner-Morro A, et al
Nat Commun 2020 09;11(1):4420