3D-EM

Cellular electron microscopy has been a key technique for understanding the cell’s core elements and mechanisms since the 1950s. Electron microscopic methods have laid the foundation for a number of Nobel Prizes and have given us much of the basic understanding of human cells’ overall structure, physiology and mechanisms—basically the foundation of all modern medicine.

In recent years, electron microscopy has undergone extremely rapid development through a combination of significantly better optics, sample processing, detectors and image processing. The development has given rise to the so-called “Resolution Revolution,” which in recent years has gradually increased the average resolution of biological preparations significantly. Part of the discoveries and methodology resulted in a Nobel Prize to Richard Henderson, Jaques Dubochet and Joachim Frank. At the beginning of this revolution, a visionary decision of the then Karolinska Institutet’s (KI’s) leadership was made to build premises for a future electron microscopy center nearby the Biomedicum building at the KI Solna campus. These premises have now been built specifically for high-end biomedical electron microscopy. During 2019-2020 we will equip the newly built KI 3D-EM center with the best possible equipment and thereby enable a much broader group of KI researchers to utilize the enormous advances in electron microscopy in recent years.

Picture of microscope Krios G3i and the Talos 120C

Instruments available

Electron microscopes

Available for use:

120 kV LaB6 microscope: Talos 120C G2 with Ceta-D detector

300 kV FEG microscope: Krios G3i with Gatan K3 detector and Ceta-D detector.
 

Under commissioning:

Aquilos 2 cryo-FIB-SEM 

Leica SP8 cryo-confocal microscope

 

Sample preparation

Available:

  • Leica Microtome 1
  • Leica Microtome 2
  • Vitrobot Mk 1
  • Vitrobot Mk 4
  • Glow discharger
  • Plasma cleaner

Access and booking

Please contact facility manager Sergej Masich who will send a link to our temporary booking system. We will utilise the central KI core-facility booking system once that is available.

Teaching and training

We will arrange courses and workshops. In the beginning, teaching and hands-on-training on specimen preparation and microscope operation is provided to smaller groups or on a one-to-one training. Please contact Sergej.Masich@ki.se for a booking link.

Prices for service

Prices for service and access for equipment currently available:

  1. Training is free for KI personnel. However, the microscope time is charged at normal rate.
  2. Talos 120C G2 cost 200 SEK per hour with minimal booking time of 3 hrs.
  3. Krios G3i with K3 cost 5000 SEK per 24h slot or 2000 SEK per 3h Autogrid screening slot.
  4. Consultant work, assistance outside training or training non-KI personnel 550 SEK per hour.
  5. Usage of sample preparation equipment 100 SEK per hour with minimal booking time 2 hrs.

 

Pricing for other academic access/training is twice the prices stated above (except hourly staff time charge). Since the internal prices are subsidized by KI they are reserved for KI groups only with personnel that are employed by KI and where all accumulated costs are internally billed.  Furthermore, only KI personnel may be the main operators of the Krios so staff setup time will typically need to be added to the total cost of access for external academic users. KI personnel are defined as employed by KI or associated hospitals and where all accumulated costs are internally billed i.e. not to clinics or other universities. All prices are subject to local KI overhead (currently 22.5%). For industry access, please contact us.

Advisory board

Stefan Eriksson (chair) Lecturer senior, Department of Physiology and Pharmacology

Martin Hällberg Senior researcher, Department of Cell and Molecular Biology

Björn Högberg Docent, Department of Medical Biochemistry and Biophysics

Luca Jovine Professor, Department of Biosciences and Nutrition

Lennart Nilsson Professor, Department of Biosciences and Nutrition

Gunter Schneider Professor, Department of Medical Biochemistry and Biophysics

Oleg Shupliakov Professor, Department of Neuroscience

Contact us

Martin Hällberg

Facility director

Sergej Masich

Facility manager

Publications resulting from 3D-EM facility use

An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction.
Hanke L, Vidakovics Perez L, Sheward DJ, Das H, Schulte T, Moliner-Morro A, et al
Nat Commun 2020 09;11(1):4420

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2
Tânia F. Custódio, Hrishikesh Das, Daniel J Sheward, Leo Hanke, Samuel Pazicky, Joanna Pieprzyk, Michèle Sorgenfrei, Martin Schroer, Andrey Gruzinov, Cy Jeffries, Melissa Graewert, Dmitri Svergun, Nikolay Dobrev, Kim Remans, Markus A. Seeger, Gerald M McInerney, Ben Murrell*, B. Martin Hällberg*, Christian Löw*
BioRxiv 2020