FENO - our offer
The Morphological Phenotype Analysis Core Facility (FENO) at Campus Flemingsberg, is a well-established core facility in service since 2002. FENO provides professional research support in animal experiments, including histopathology, a wide range of staining, digitilization and pathological analysis with highly specialized comepetence in genetically modified mice phenotyping. Other than mice, FENO also provides services on the pathological analysis for several other animal species.
Our service
- Project consultation on experimental design, animal models, necropsy and histopathology protocols, breeding schemes, etc.
- Necropsy and histopathology services for pathological feature characterization in tissues and organs, with a focus on species differences.
- Tissue processing and sectioning using microtome/cryotome, staining, and pathological analysis.
- Immunohistochemistry on frozen/paraffin embedded tissues.
- Imaging data registration of pathological analysis including light- and fluorescence microscopy.
- Slide digitalization and image analysis.
Our method and techniques
| Method | Instrument/technique |
|---|---|
| Study design, planning, autopsy | FENO consultation |
| Tissue collection, registration | iLab |
| Tissue dehydration /paraffinization | Automatic/manual |
| Sectioning: paraffin | 4 x Thermo microtome |
| Sectioning: cryo | Leica cryotome |
| Histochemical routine staining (HE) | TISSUE-TEK /manual |
| Histochemical special staining | Tiziano robot/manual |
| Immunohistochemical staining (chromogenic /fluorescent/multiplex) | Leica Bond RXm robot/ manual |
| RNA in situ hybridization | Leica bond RXm -RNA scope |
| Histologic image documentation | 2x microscope camera (Nikon) 3D histech section scanner 20x ZEISS Axioscan 7 scanner (bright field, polarization and fluorescence function) |
| Online sharing of images/scanned sections | FENO server |
| Histopathological analysis, support for image analysis | Certified pathology |
Histological stains
- H&E
- Special Stains
- Special stains and immunostaining multiplexing
Immunostaining
- Automated Immunohistochemistry (IHC) – Single and Dual
- Automated Immunofluorescence (IF)
- Automated in situ hybridization (ISH/FISH)
Four examples of staining: H&E, Sirius Red, PAS and ORO.