FENO - our offer
The FENO Histological Core Facility at Campus Flemingsberg, is a well-established core facility in service since 2002. FENO provides professional research support in animal experiments, including histopathology, a wide range of stainings, IHC, IF, Opal and RNAScope and digitalization.
Our service
- Histopathology services in tissues and organs for different species.
- Tissue processing and sectioning using microtome/cryotome.
- Routine and special stainings.
- Immunohistochemistry on frozen/paraffin embedded tissues.
- Immunohistochemistry on frozen/paraffin embedded tissues using automated Leica BOND RXm.
- RNAScope (InSitu hybridization) on frozen/paraffin embedded tissues.
- Bright field scanning - slide digitalization.
Our methods and techniques
Method | Instrument/technique |
---|---|
Study design, planning | FENO consultation |
Registration | iLab |
Tissue dehydration /paraffinization | Automatic |
Sectioning: paraffin | 4 x Thermo microtome |
Sectioning: cryo | Leica cryotome |
Histochemical routine staining (HE) | Manual |
Histochemical special staining | Tiziano robot/manual |
Immunohistochemical staining (chromogenic /fluorescent/multiplex) | Leica Bond RXm robot/ manual |
RNA Scope in situ hybridization | Leica bond RXm/manual-RNA scope |
Histologic image scanning | ZEISS Axioscan 7 scanner (bright field, polarization and fluorescence function) Pannoramic D histech section scanner 20x |
Online sharing of images/scanned sections | OneDrive server |
Study documentation | KI ELN - The electronic notebook |
Histological stains
- H&E
- Special Stainings: PAS, Sirius Red, Oil Red O
Immunostaining
- Automated Immunohistochemistry (IHC) – Single and Dual
- Automated Immunofluorescence (IF)
- Opal 4 and 7-Color
- Automated in situ hybridization (ISH/FISH)

Four examples of staining: H&E, Sirius Red, PAS and ORO.