FENO - our offer

The FENO Histological Core Facility at Campus Flemingsberg, is a well-established core facility in service since 2002. FENO provides professional research support in animal experiments, including histopathology, a wide range of stainings, IHC, IF, Opal and RNAScope and digitalization.

Our service

  • Histopathology services in tissues and organs for different species.
  • Tissue processing and sectioning using microtome/cryotome.
  • Routine and special stainings.
  • Immunohistochemistry on frozen/paraffin embedded tissues.
  • Immunohistochemistry on frozen/paraffin embedded tissues using automated Leica BOND RXm.
  • RNAScope (InSitu hybridization) on frozen/paraffin embedded tissues.
  • Bright field scanning - slide digitalization.

Our methods and techniques

Method and techniques at FENO
MethodInstrument/technique
Study design, planningFENO consultation
RegistrationiLab
Tissue dehydration /paraffinizationAutomatic
Sectioning: paraffin4 x Thermo microtome
Sectioning: cryoLeica cryotome
Histochemical routine staining (HE)Manual
Histochemical special stainingTiziano robot/manual
Immunohistochemical staining (chromogenic /fluorescent/multiplex)Leica Bond RXm robot/ manual
RNA Scope in situ hybridizationLeica bond RXm/manual-RNA scope
Histologic image scanningZEISS Axioscan 7 scanner (bright field, polarization and fluorescence function)
Pannoramic D histech section scanner 20x
Online sharing of images/scanned sectionsOneDrive server
Study documentationKI ELN - The electronic notebook

Histological stains

  • H&E
  • Special Stainings: PAS, Sirius Red, Oil Red O

Immunostaining

  • Automated Immunohistochemistry (IHC) – Single and Dual
  • Automated Immunofluorescence (IF)
  • Opal 4 and 7-Color
  • Automated in situ hybridization (ISH/FISH)
Four examples of staining at FENO.
Four examples of staining: H&E, Sirius Red, PAS and ORO.