CFG - Our offer

CRISPR Functional Genomics (CFG) provides CRISPR-based services for functional genomics, covering both pooled screening and precision genome editing.

Service areas

Pooled screening

Pooled CRISPR screens are scalable and cost-effective tools for discovery and functional studies. CFG has specialized in pooled screening since 2017, including the development of in-house barcoded guide RNA libraries.

We offer screens using different nuclease-proficient Cas enzymes (Cas9, Cas12, and Cas13), as well as nuclease-deficient versions of these enzymes fused to transcriptional repressors (CRISPRi), activators (CRISPRa), and base editors for scanning mutagenesis. Screens can target protein-coding genes as well as lncRNAs, miRNAs, enhancers, promoters, and other non-coding or regulatory genomic elements.

CFG designs and produces custom guide libraries ranging from dozens to more than 150,000 guides. Screen readout can be based on phenotypic selection, single-cell RNA sequencing (Perturb-seq, CROP-seq), or optical phenotyping (in development).

Service portfolio

  • Creation of stable Cas9, Cas12, or Cas13 expressing cell lines (lentivirus, piggyBac)
  • Design and production of pooled, barcoded sgRNA libraries (100–150,000 guides), from design to packaged lentivirus
  • Genome-wide or custom CRISPR based screens in a pooled format
  • Pooled screens with single-cell transcriptomic readout (Perturb-Seq, CROP-Seq; in collaboration with NGI)
  • Scanning mutagenesis using base editors to study protein function and drug–target interactions

Precision editing

CFG offers targeted genome editing in the cell type of choice, including defined knockouts, knock-ins, point mutations, and insertion of small or large DNA fragments. Projects range from strategy design to delivery of edited cell pools or fully genotyped clones.

We perform precision genome engineering in established cell lines, primary cells, and embryonic stem cells, and design CRISPR reagents for the generation of genetically modified mouse models.

Applications include disease-relevant model systems, reporter cell lines, validation of screening hits, and generation of isogenic cell line panels.

Our toolbox includes HDR-based editing, base editing, prime editing, and strategies for larger deletions or targeted insertions.

  • Service portfolio
  • Strategy design and feasibility assessment
  • Optimization of delivery methods and delivery into target cells
  • Efficiency quantification using droplet digital PCR or other methods
  • Clone expansion and genotyping
  • Design of reagents for genetically modified mouse models

Cost model and pricing

CFG is an academic, not-for-profit research unit. Charges follow Karolinska Institutet’s full cost model for commissioned research. Academic projects are co-funded by CFG, while industry and private clients are charged at full cost. Initial discussions, project scoping, and quotations are provided free of charge.