CFG - Our offer
CRISPR Functional Genomics (CFG) provides CRISPR related services, both in the form of pooled screening and precision editing.
Service areas
Pooled screening
Pooled CRISPR screens are customizable, scalable, and cost-effective tools in both de novo discovery and functional exploration of phenotypes of interest. CFG has been specializing in applying and further developing this technology since 2017, incorporating in-house methods such as barcoded guide libraries.
CFG offers screening with Cas9, Cas12, and Cas13, as well as with fusions of these enzymes to transcriptional repressors (CRISPR-inhibition, CRISPR-i), activators (CRISPR-activation, CRISPR-a), and base-editors (scanning mutagenesis).
CFG designs and creates any guide library requested, from hundreds to more than hundred thousand sequences. For screen deconvolution, we use either phenotypic selection (viability, reporter expression, etc), single cell RNASeq (Perturb-Seq, CROP-Seq) or optical phenotyping (in development).
Service portfolio
- Creation of stable Cas9/Cas12/Cas13 expressing cell lines (lentivirus, piggyBac)
- Creation of pooled, barcoded sgRNA libraries (100 – 150,000 guides), from design to packaged lentivirus
- Genome-wide/custom CRISPR-Cas9 KO, inhibition and activation screens for protein coding genes, miRNAs, lncRNAs, and regulatory genetic elements
- Pooled screens with single-cell RNASeq readout (in collaboration with NGI)
- Scanning mutagenesis with base-editors to characterize protein-protein or drug-target interactions
Precision editing
Our precision editing branch offers specific knock-outs and knock-ins in your cell type of choice. We can introduce point mutations or insert smaller or larger DNA fragments. From strategy design to either an edited cell pool or genotyped clones.
Precision genome engineering refers to the defined and precise modification of a genomic locus. CFG performs such modifications in cell lines, primary cells and ESCs, and designs CRISPR reagents for the creation of genetically modified mice.
Application examples are the creation of disease-representative model systems, of reporter cell lines for high-throughput screens or other applications, the validation of hits from such screens, creation of isogenic cell lines, and many more.
CFG has a rich toolbox for creating knock-outs, knock-ins, small mutations, or large deletions, including HDR-based editing, base-editing and prime-editing.
Service portfolio
- Strategy design
- Optimization of delivery method and delivery into target cells
- Quantifications by droplet digital PCR
- Clone growing and genotyping
- Genetically modified mice
Cost model and pricing
CFG is an academic, not-for-profit research unit. Charges are based on Karolinska Institutet’s full cost model for commissioned research.
Academic projects are generously co-funded by CFG.
Industry and private clients are charged at full cost. Initial discussions and development of a feasible project plan and a quote are free of charge.