Bernhard Schmierer
Researcher
E-mail: bernhard.schmierer@ki.se
Visiting address: Biomedicum Solnavägen 9, 17165 Solna
Postal address: C2 Medicinsk biokemi och biofysik, C2 Chemical Biology and Genome engineering Bernhard, 171 77 Stockholm
About me
- I am heading CRISPR Functional Genomics (CFG), formerly High Throughput
Genome Engineering, a National SciLifeLab Facility offering massively
parallel CRISPR-Cas applications to the Swedish and international research
communities.
Research
- Pooled CRISPR-Cas screening approaches enables parallel interrogation of
thousands of protein coding genes, lncRNAs or other genetic elements for
involvement in biological processes of interest. CFG provides access to
verified lentiviral CRISPR guide libraries for whole genome and targeted
loss- and gain of function studies (CRISPR knock-out, CRISPR inhibition,
CRISPR activation, base-editing mutagenesis screens). CFG offers support from
experimental design to data analysis.
Articles
- Journal article: NUCLEIC ACIDS RESEARCH. 2025;53(16):gkaf841Fan C; Cats D; Selle M; Khorosjutina O; Dhanjal S; Schmierer B; Mei H; ten Dijke P; Wang Q
- Journal article: PLOS BIOLOGY. 2025;23(8):e3003297Sait MRB; Jachmann LH; Turkoz G; Milivojevic M; Llorente-Saez C; Dhanjal S; Schumacher F; Henriksson S; Vegesna NVG; Seddik N; Chaban A; Mohanty P; Olander M; Muraleedharan S; Azadeh SF; Kleuser B; Schmierer B; Sixt BS
- Journal article: CRISPR JOURNAL. 2025;8(3):216-223Henkel E; Li Z; Uvehag D; Schmierer B; Henkel M; Wermeling F
- Journal article: NATURE COMMUNICATIONS. 2025;16(1):2890Fan C; Wang Q; Krijger PHL; Cats D; Selle M; Khorosjutina O; Dhanjal S; Schmierer B; Mei H; de Laat W; ten Dijke P
- Journal article: CANCERS. 2024;16(13):2437Issa II; Due H; Brondum RF; Veeravakaran V; Haraldsdottir H; Sylvester C; Brogaard A; Dhanjal S; Schmierer B; Dybkaer K
- Journal article: EJNMMI RADIOPHARMACY AND CHEMISTRY. 2024;9(1):32Durinova A; Smutna L; Barta P; Kamaraj R; Smutny T; Schmierer B; Pavek P; Trejtnar F
- Journal article: CANCER RESEARCH. 2024;84(6):2025Dam N; Harryvan TJ; Schmierer B; Hawinkels LJAC; Kemp V
- Article: NATURE COMMUNICATIONS. 2023;14(1):5024Moro CF; Geyer N; Harrizi S; Hamidi Y; Soderqvist S; Kuznyecov D; Tidholm Qvist E; Salmonson Schaad M; Hermann L; Lindberg A; Heuchel RL; Martin-Bernabe A; Dhanjal S; Navis AC; Villard C; del Valle AC; Bozoky L; Sparrelid E; Dirix L; Strell C; Ostman A; Schmierer B; Vermeulen PB; Engstrand J; Bozoky B; Gerling M
- Journal article: CANCER RESEARCH. 2023;83(7):3778Weiss S; Lord A; Schmierer B; Rovsing AB; Thomsen EA; Mikkelsen JG; Ulhoi B; Pedersen JS; Borre M; Sorensen KD
- Article: FRONTIERS IN ONCOLOGY. 2022;12:852980Gubat J; Selvaraju K; Sjoestrand L; Kumar Singh D; Turkina MV; Schmierer B; Sabatier P; Zubarev RA; Linder S; D'Arcy P
- Article: ELIFE. 2021;10:e75415Reint G; Li Z; Labun K; Keskitalo S; Soppa I; Mamia K; Tolo E; Szymanska M; Meza-Zepeda LA; Lorenz S; Cieslar-Pobuda A; Hu X; Bordin DL; Staerk J; Valen E; Schmierer B; Varjosalo M; Taipale J; Haapaniemi E
- Article: JOURNAL OF CELL SCIENCE. 2019;132(14):jcs234039Miller DSJ; Schmierer B; Hill CS
- Article: NATURE COMMUNICATIONS. 2018;9(1):3664Palin K; Pitkanen E; Turunen M; Sahu B; Pihlajamaa P; Kivioja T; Kaasinen E; Valimaki N; Hanninen UA; Cajuso T; Aavikko M; Tuupanen S; Kilpivaara O; van den Berg L; Kondelin J; Tanskanen T; Katainen R; Grau M; Rauanheimo H; Plaketti R-M; Taira A; Sulo P; Hartonen T; Dave K; Schmierer B; Botla S; Sokolova M; Vaharautio A; Gladysz K; Ongen H; Dermitzakis E; Bramsen JB; Orntoft TF; Andersen CL; Ristimaki A; Lepisto A; Renkonen-Sinisalo L; Mecklin J-P; Taipale J; Aaltonen LA
- Article: NATURE MEDICINE. 2018;24(7):927-930Haapaniemi E; Botla S; Persson J; Schmierer B; Taipale J
- Article: MOLECULAR SYSTEMS BIOLOGY. 2017;13(10):945Schmierer B; Botla SK; Zhang J; Turunen M; Kivioja T; Taipale J
- Article: NATURE COMMUNICATIONS. 2015;6:10050Morgunova E; Yin Y; Jolma A; Dave K; Schmierer B; Popov A; Eremina N; Nilsson L; Taipale J
- Article: SCIENCE SIGNALING. 2013;6(305):ra106Vizan P; Miller DSJ; Gori I; Das D; Schmierer B; Hill CS
- Article: CELL. 2013;154(4):801-813Yan J; Enge M; Whitington T; Dave K; Liu J; Sur I; Schmierer B; Jolma A; Kivioja T; Taipale M; Taipale J
- Article: BIOPHYSICAL JOURNAL. 2013;104(12):2595-2606Zhang T; Oliveira RA; Schmierer B; Novak B
- Article: OPEN BIOLOGY. 2011;1(3):110009Zhang T; Schmierer B; Novak B
- Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2011;286(9):7648-7660Yang M; Ge W; Chowdhury R; Claridge TDW; Kramer HB; Schmierer B; McDonough MA; Gong L; Kessler BM; Ratcliffe PJ; Coleman ML; Schofield CJ
- Article: INTEGRATIVE BIOLOGY. 2011;3(3):197-207Gonzalez-Perez V; Schmierer B; Hill CS; Sear RP
- Article: BMC SYSTEMS BIOLOGY. 2010;4:139Schmierer B; Novak B; Schofield CJ
- Article: DEVELOPMENT. 2008;135(17):2927-2937Batut J; Schmierer B; Cao J; Raftery LA; Hill CS; Howell M
- Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2008;105(18):6608-6613Schmierer B; Tournier AL; Bates PA; Hill CS
- Article: JOURNAL OF CELL SCIENCE. 2006;119(12):2435-2443Kim S-H; Rowe J; Fujii H; Jones R; Schmierer B; Kong B-W; Kuchler K; Foster D; Ish-Horowicz D; Peters G
- Article: MOLECULAR AND CELLULAR BIOLOGY. 2005;25(22):9845-9858Schmierer B; Hill CS
- Article: JOURNAL OF CELL SCIENCE. 2004;117(18):4113-4125Nicolás FJ; De Bosscher K; Schmierer B; Hill CS
- Article: BIOLOGY OF REPRODUCTION. 2004;70(5):1493-1499Schuster MK; Schmierer B; Shkumatava A; Kuchler K
- Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2003;278(23):21197-21203Schmierer B; Schuster MK; Shkumatava A; Kuchler K
- Article: BIOLOGY OF REPRODUCTION. 2003;68(2):620-627Schmierer B; Schuster MK; Shkumatava A; Kuchler K
- Article: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 2000;274(2):542-547Oehler R; Schmierer B; Zellner M; Prohaska R; Roth E
- Article: FASEB JOURNAL. 2000;14(9):1093-1100Gornikiewicz A; Sautner T; Brostjan C; Schmierer B; Függer R; Roth E; Mühlbacher F; Bergmann M
- Show more
All other publications
- Preprint: BIORXIV. 2024Sait MRB; Jachmann LH; Milivojevic M; Llorente-Sáez C; Dhanjal S; Schumacher F; Henriksson S; Vegesna NVG; Chaban A; Mohanty P; Ölander M; Muraleedharan S; Azadeh SF; Kleuser B; Schmierer B; Sixt BS
- Preprint: BIORXIV. 2024Söderqvist S; Viljamaa A; Geyer N; Strell C; Hekmati N; Engstrand J; Sparrelid E; Salmén C; Heuchel R; Zacharouli A; Ghorbani P; Harrizi S; Hamidi Y; Khorosjutina O; Milanova S; Schmierer B; Bozóky B; Moro CF; Gerling M
- Conference publication: CANCER RESEARCH. 2022;82(22):5Laise P; Turunen M; Maurer HC; Garcia AC; Elyada E; Schmierer B; Tomassoni L; Worley J; Alvarez MJ; Kesner J; Tan X; Tagore S; Fernandez EC; Wong K; Wang ALE; Ge S; Iuga AC; Griffin AT; Wong W; Manji GA; Notta F; Tuveson DA; Olive KPP; Califano A
- Conference publication: MOLECULAR THERAPY. 2022;30(4):267Reint G; Li Z; Labun K; Keskitalo S; Soppa I; Mamia K; Tolo E; Szymanska M; Meza-Zepeda L; Lorenz S; Cieslar-Pobuda A; Hu X; Bordin D; Staerk J; Valen E; Schmierer B; Varjosalo M; Taipale J; Haapaniemi E
- Book chapter: GENOME EDITING IN DRUG DISCOVERY. 2022;p. 151-164Persson J; Schmierer B
- Preprint: BIORXIV. 2020Laise P; Turunen M; Garcia AC; Tomassoni L; Maurer C; Elyada E; Schmierer B; Worley J; Kesner J; Tan X; Fernandez EC; Wong K; Wasko U; Tagore S; Wang A; Ge S; Iuga A; Griffin A; Wong W; Manji G; Alvarez M; Notta F; Tuveson D; Olive K; Califano A
- Review: COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL. 2020;18:2237-2246Iyer VS; Jiang L; Shen Y; Boddul SV; Panda SK; Kasza Z; Schmierer B; Wermeling F
- Letter: MOLECULAR SYSTEMS BIOLOGY. 2019;15(8):e9059Haapaniemi E; Botla S; Persson J; Schmierer B; Taipale J
- Letter: SCIENCE SIGNALING. 2014;7(344):lc2Vizan P; Miller DSJ; Schmierer B; Hill CS
- Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 311-314Schmierer B
- Corrigendum: INTEGRATIVE BIOLOGY. 2011;3(12):1233Gonzalez-Perez V; Schmierer B; Hill CS; Sear RP
- Review: NATURE REVIEWS MOLECULAR CELL BIOLOGY. 2007;8(12):970-982Schmierer B; Hill CS
- Book chapter: SMAD SIGNAL TRANSDUCTION: PROTEINS AND CELL REGULATION. 2006;p. 193-213Schmierer B; Hill CS
Grants
- Swedish Research Council1 January 2023 - 31 December 2025One of the important unresolved questions in biomedicine is our inability to conditionally modulate endogenous gene expression limited to naturally expressing cells. This inability hinders association of gene expression levels to human disease and development of novel treatments, especially in tissues where expression site and levels are critically important such as the brain. I have made substantial progress towards solving this problem by showing that it is possible to conditionally increase endogenous gene, such as neurotrophic factor GDNF expression by conditionally replacing its 3´UTR. Demonstrating the power to discover I used patient analysis and this approach to identify increased GDNF as a potential driver of schizophrenia in a sub-group of patients and identified adenosine receptor Adora2a and GDNF receptor RET as potential new precision medicine to treat GDNFhigh sub-group of schizophrenia. Here I propose further studies to proceed towards clinical trials. Next, using new CRISPR-Cas9 tiling library screen I found that it is possible to identify single gRNAs which either enhance or reduce expression from endogenous mRNA via 3´UTR editing, establishing new research and treatment tool. Now we can test my hypothesis that increasing endogenous autophagy, protein folding and neurotrophic function may reverse the progression of currently untreatable Parkinson´s disease better than current ectopic gene expression tools.
- Swedish Research Council1 January 2021 - 31 December 2023