Bernhard Schmierer

About me
Publications
Grants
Public outreach and news

About me

I am heading CRISPR Functional Genomics (CFG), formerly High Throughput
Genome Engineering, a National SciLifeLab Facility offering massively
parallel CRISPR-Cas applications to the Swedish and international research
communities.

Research

Pooled CRISPR-Cas screening approaches enables parallel interrogation of
thousands of protein coding genes, lncRNAs or other genetic elements for
involvement in biological processes of interest. CFG provides access to
verified lentiviral CRISPR guide libraries for whole genome and targeted
loss- and gain of function studies (CRISPR knock-out, CRISPR inhibition,
CRISPR activation, base-editing mutagenesis screens). CFG offers support from
experimental design to data analysis.

Articles

Journal article: CANCERS. 2024;16(13):2437
CRISPR-Cas9 Knockout Screens Identify DNA Damage Response Pathways and BTK as Essential for Cisplatin Response in Diffuse Large B-Cell Lymphoma
Issa II; Due H; Brondum RF; Veeravakaran V; Haraldsdottir H; Sylvester C; Brogaard A; Dhanjal S; Schmierer B; Dybkaer K
Journal article: EJNMMI RADIOPHARMACY AND CHEMISTRY. 2024;9(1):32
Radiolabeled 15-mer peptide internalization is mediated by megalin (LRP2 receptor) in a CRISPR/Cas9-based LRP2 knockout human kidney cell model
Durinova A; Smutna L; Barta P; Kamaraj R; Smutny T; Schmierer B; Pavek P; Trejtnar F
Journal article: CANCER RESEARCH. 2024;84(6):2025
Zeb1 downregulation sensitizes pancreatic cancer-associated fibroblasts to killing by oncolytic reovirus through upregulation of the reovirus receptor junction adhesion molecule A
Dam N; Harryvan TJ; Schmierer B; Hawinkels LJAC; Kemp V
Article: NATURE COMMUNICATIONS. 2023;14(1):5024
An idiosyncratic zonated stroma encapsulates desmoplastic liver metastases and originates from injured liver
Moro CF; Geyer N; Harrizi S; Hamidi Y; Soderqvist S; Kuznyecov D; Tidholm Qvist E; Salmonson Schaad M; Hermann L; Lindberg A; Heuchel RL; Martin-Bernabe A; Dhanjal S; Navis AC; Villard C; del Valle AC; Bozoky L; Sparrelid E; Dirix L; Strell C; Ostman A; Schmierer B; Vermeulen PB; Engstrand J; Bozoky B; Gerling M
Journal article: CANCER RESEARCH. 2023;83(7):3778
Genome-Scale CRISPRa and CRISPRi screening for lncRNA drivers of prostate cancer progression
Weiss S; Lord A; Schmierer B; Rovsing AB; Thomsen EA; Mikkelsen JG; Ulhoi B; Pedersen JS; Borre M; Sorensen KD
Article: FRONTIERS IN ONCOLOGY. 2022;12:852980
Comprehensive Target Screening and Cellular Profiling of the Cancer-Active Compound b-AP15 Indicate Abrogation of Protein Homeostasis and Organelle Dysfunction as the Primary Mechanism of Action
Gubat J; Selvaraju K; Sjoestrand L; Kumar Singh D; Turkina MV; Schmierer B; Sabatier P; Zubarev RA; Linder S; D'Arcy P
Article: ELIFE. 2021;10:e75415
Rapid genome editing by CRISPR-Cas9-POLD3 fusion
Reint G; Li Z; Labun K; Keskitalo S; Soppa I; Mamia K; Tolo E; Szymanska M; Meza-Zepeda LA; Lorenz S; Cieslar-Pobuda A; Hu X; Bordin DL; Staerk J; Valen E; Schmierer B; Varjosalo M; Taipale J; Haapaniemi E
Article: JOURNAL OF CELL SCIENCE. 2019;132(14):jcs234039
TGF-β family ligands exhibit distinct signalling dynamics that are driven by receptor localisation
Miller DSJ; Schmierer B; Hill CS
Article: NATURE COMMUNICATIONS. 2018;9(1):3664
Contribution of allelic imbalance to colorectal cancer
Palin K; Pitkanen E; Turunen M; Sahu B; Pihlajamaa P; Kivioja T; Kaasinen E; Valimaki N; Hanninen UA; Cajuso T; Aavikko M; Tuupanen S; Kilpivaara O; van den Berg L; Kondelin J; Tanskanen T; Katainen R; Grau M; Rauanheimo H; Plaketti R-M; Taira A; Sulo P; Hartonen T; Dave K; Schmierer B; Botla S; Sokolova M; Vaharautio A; Gladysz K; Ongen H; Dermitzakis E; Bramsen JB; Orntoft TF; Andersen CL; Ristimaki A; Lepisto A; Renkonen-Sinisalo L; Mecklin J-P; Taipale J; Aaltonen LA
Article: NATURE MEDICINE. 2018;24(7):927-930
CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response
Haapaniemi E; Botla S; Persson J; Schmierer B; Taipale J
Article: MOLECULAR SYSTEMS BIOLOGY. 2017;13(10):945
CRISPR/Cas9 screening using unique molecular identifiers
Schmierer B; Botla SK; Zhang J; Turunen M; Kivioja T; Taipale J
Article: NATURE COMMUNICATIONS. 2015;6:10050
Structural insights into the DNA-binding specificity of E2F family transcription factors
Morgunova E; Yin Y; Jolma A; Dave K; Schmierer B; Popov A; Eremina N; Nilsson L; Taipale J
Article: SCIENCE SIGNALING. 2013;6(305):ra106
Controlling Long-Term Signaling: Receptor Dynamics Determine Attenuation and Refractory Behavior of the TGF-β Pathway
Vizan P; Miller DSJ; Gori I; Das D; Schmierer B; Hill CS
Article: CELL. 2013;154(4):801-813
Transcription Factor Binding in Human Cells Occurs in Dense Clusters Formed around Cohesin Anchor Sites
Yan J; Enge M; Whitington T; Dave K; Liu J; Sur I; Schmierer B; Jolma A; Kivioja T; Taipale M; Taipale J
Article: BIOPHYSICAL JOURNAL. 2013;104(12):2595-2606
Dynamical Scenarios for Chromosome Bi-orientation
Zhang T; Oliveira RA; Schmierer B; Novak B
Article: OPEN BIOLOGY. 2011;1(3):110009
Cell cycle commitment in budding yeast emerges from the cooperation of multiple bistable switches
Zhang T; Schmierer B; Novak B
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2011;286(9):7648-7660
Asparagine and Aspartate Hydroxylation of the Cytoskeletal Ankyrin Family Is Catalyzed by Factor-inhibiting Hypoxia-inducible Factor
Yang M; Ge W; Chowdhury R; Claridge TDW; Kramer HB; Schmierer B; McDonough MA; Gong L; Kessler BM; Ratcliffe PJ; Coleman ML; Schofield CJ
Article: INTEGRATIVE BIOLOGY (UNITED KINGDOM). 2011;3(3):197-207
Studying Smad2 intranuclear diffusion dynamics by mathematical modelling of FRAP experiments
Gonzalez-Perez V; Schmierer B; Hill CS; Sear RP
Article: BMC SYSTEMS BIOLOGY. 2010;4:139
Hypoxia-dependent sequestration of an oxygen sensor by a widespread structural motif can shape the hypoxic response - a predictive kinetic model
Schmierer B; Novak B; Schofield CJ
Article: DEVELOPMENT. 2008;135(17):2927-2937
Two highly related regulatory subunits of PP2A exert opposite effects on TGF-β/Activin/Nodal signalling
Batut J; Schmierer B; Cao J; Raftery LA; Hill CS; Howell M
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2008;105(18):6608-6613
Mathematical modeling identifies Smad nucleocytoplasmic shuttling as a dynamic signal-interpreting system
Schmierer B; Tournier AL; Bates PA; Hill CS
Article: JOURNAL OF CELL SCIENCE. 2006;119(12):2435-2443
Upregulation of chicken p15^INK4b at senescence and in the developing brain
Kim S-H; Rowe J; Fujii H; Jones R; Schmierer B; Kong B-W; Kuchler K; Foster D; Ish-Horowicz D; Peters G
Article: MOLECULAR AND CELLULAR BIOLOGY. 2005;25(22):9845-9858
Kinetic analysis of smad nucleocytoplasmic shuttling reveals a mechanism for transforming growth factor β-dependent nuclear accumulation of Smads
Schmierer B; Hill CS
Article: JOURNAL OF CELL SCIENCE. 2004;117(18):4113-4125
Analysis of Smad nucleocytoplasmic shuttling in living cells
Nicolás FJ; De Bosscher K; Schmierer B; Hill CS
Article: BIOLOGY OF REPRODUCTION. 2004;70(5):1493-1499
Activin A and follicle-stimulating hormone control tight junctions in avian granulosa cells by regulating occludin expression
Schuster MK; Schmierer B; Shkumatava A; Kuchler K
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2003;278(23):21197-21203
Activin A signaling induces Smad2, but not Smad3, requiring protein kinase A activity in granulosa cells from the avian ovary
Schmierer B; Schuster MK; Shkumatava A; Kuchler K
Article: BIOLOGY OF REPRODUCTION. 2003;68(2):620-627
Activin and follicle-stimulating hormone signaling are required for long-term culture of functionally differentiated primary granulosa cells from the chicken ovary
Schmierer B; Schuster MK; Shkumatava A; Kuchler K
Article: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 2000;274(2):542-547
Endothelial cells downregulate expression of the 70 kDa heat shock protein during hypoxia
Oehler R; Schmierer B; Zellner M; Prohaska R; Roth E
Article: THE FASEB JOURNAL. 2000;14(9):1093-1100
Catecholamines up-regulate lipopolysaccharide-induced IL-6 production in human microvascular endothelial cells
Gornikiewicz A; Sautner T; Brostjan C; Schmierer B; Függer R; Roth E; Mühlbacher F; Bergmann M
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All other publications

Preprint: BIORXIV. 2024
A genome-wide genetic screen identified targets for destabilizing the parasitophorous vacuole of Chlamydia trachomatis
Sait MRB; Jachmann LH; Milivojevic M; Llorente-Sáez C; Dhanjal S; Schumacher F; Henriksson S; Vegesna NVG; Chaban A; Mohanty P; Ölander M; Muraleedharan S; Azadeh SF; Kleuser B; Schmierer B; Sixt BS
Preprint: BIORXIV. 2024
A distinctive tumor compartment in pancreatic lobules defined by nascent stroma and classical tumor cell phenotype
Söderqvist S; Viljamaa A; Geyer N; Strell C; Hekmati N; Engstrand J; Sparrelid E; Salmén C; Heuchel R; Zacharouli A; Ghorbani P; Harrizi S; Hamidi Y; Khorosjutina O; Milanova S; Schmierer B; Bozóky B; Moro CF; Gerling M
Book chapter: GENOME EDITING IN DRUG DISCOVERY. 2022;p. 151-164
Sequence Diversification Screens with CRISPR‐Cas9‐Guided Base Editors
Persson J; Schmierer B
Conference publication: CANCER RESEARCH. 2022;82(22):5
Pancreatic cancer comprises co-existing transcriptional states regulated by distinct master regulator programs.
Laise P; Turunen M; Maurer HC; Garcia AC; Elyada E; Schmierer B; Tomassoni L; Worley J; Alvarez MJ; Kesner J; Tan X; Tagore S; Fernandez EC; Wong K; Wang ALE; Ge S; Iuga AC; Griffin AT; Wong W; Manji GA; Notta F; Tuveson DA; Olive KPP; Califano A
Conference publication: MOLECULAR THERAPY. 2022;30(4):267
Rapid Genome Editing by Cas9-POLD3 Fusion
Reint G; Li Z; Labun K; Keskitalo S; Soppa I; Mamia K; Tolo E; Szymanska M; Meza-Zepeda L; Lorenz S; Cieslar-Pobuda A; Hu X; Bordin D; Staerk J; Valen E; Schmierer B; Varjosalo M; Taipale J; Haapaniemi E
Preprint: BIORXIV. 2020
Developmental and MAPK-responsive transcription factors drive distinct malignant subtypes and genetic dependencies in pancreatic cancer
Laise P; Turunen M; Garcia AC; Tomassoni L; Maurer C; Elyada E; Schmierer B; Worley J; Kesner J; Tan X; Fernandez EC; Wong K; Wasko U; Tagore S; Wang A; Ge S; Iuga A; Griffin A; Wong W; Manji G; Alvarez M; Notta F; Tuveson D; Olive K; Califano A
Review: COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL. 2020;18:2237-2246
Designing custom CRISPR libraries for hypothesis-driven drug target discovery
Iyer VS; Jiang L; Shen Y; Boddul SV; Panda SK; Kasza Z; Schmierer B; Wermeling F
Letter: MOLECULAR SYSTEMS BIOLOGY. 2019;15(8):e9059
Reply to "CRISPR screens are feasible in TP53 wild-type cells"
Haapaniemi E; Botla S; Persson J; Schmierer B; Taipale J
Letter: SCIENCE SIGNALING. 2014;7(344):lc2
Response to comment on "Controlling long-term signaling: Receptor dynamics determine attenuation and refractory behavior of the TGF-β pathway"-Smad2/3 activity does not predict the dynamics of transcription
Vizan P; Miller DSJ; Schmierer B; Hill CS
Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 29
Anoxia
Schmierer B
Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 311-314
Cell Cycle Signaling, Hypoxia
Schmierer B
Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 1055-1056
Ischemic Disease
Schmierer B
Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 1846
Reoxygenation
Schmierer B
Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 1802
PVHL
Schmierer B
Book chapter: ENCYCLOPEDIA OF SYSTEMS BIOLOGY. 2013;p. 434
c-Myc
Schmierer B
Corrigendum: INTEGRATIVE BIOLOGY (UNITED KINGDOM). 2011;3(12):1233
Studying Smad2 intranuclear diffusion dynamics by mathematical modelling of FRAP experiments (vol 3, pg 197, 2011)
Gonzalez-Perez V; Schmierer B; Hill CS; Sear RP
Review: NATURE REVIEWS MOLECULAR CELL BIOLOGY. 2007;8(12):970-982
TGFβ-SMAD signal transduction:: molecular specificity and functional flexibility
Schmierer B; Hill CS
Book chapter: SMAD SIGNAL TRANSDUCTION: PROTEINS AND CELL REGULATION. 2006;p. 193-213
Nucleocytoplasmic Shuttling of Smad Proteins
Schmierer B; Hill CS

Grants

Gene Knock Up at the post-transcriptional level to model and treat neurological disease
Swedish Research Council
1 January 2023 - 31 December 2025
Differentiation state instability and clonal selection as escape paths from anticancer therapy
Swedish Research Council
1 January 2021 - 31 December 2023
Mechanism of Smad2/3 nucleocytoplasmic shuttling
FWF Austrian Science Fund
1 June 2004 - 31 May 2006

About me

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