BSL3 Biomedicum- our offer

BSL3 Biomedicum serves a growing body of researchers at Karolinska Institutet working on risk-group 3 bacteria, parasites and viruses.

BSL3 Biomedicum

About the facility

The faciliy is located in Biomedicum quarter C9 and was inaugurated in 2018. It totals approximately 180 m2 of working space. It is is located close to the animal BSL3 facility at Astrid Fagraeus-Comparative Medicine, where the risk-group 3 pathogens handled at Biomedicum can used in animal models of infection and animal tissues processed back at Biomedicum.

BSL3 Biomedicum has two interconnected but separate barriers approved by the Swedish Work Environment Authority to handle the following risk-group 3 pathogens within its respective barriers.

Airborne suite:

  • Mycobacterium tuberculosis
  • SARS-CoV-2
  • Monkeypox virus


Non-airborne suite:

  • Chikungunya virus
  • Leishmania brasiliensis, L. donovani
  • HIV
  • Plasmodium falciparum


The facility supports various research activities integrated in its capabilities available also as a service to users. These capabilities include:                                                                                                                                                                                                             

  • High throughput antiviral and antibiotic screening 
  • Aerobiology investigative techniques
  • Large-scale propagation and concentration of risk-group 3 pathogens
  • In vitro infections 
  • 10x Genomics single-cell RNA sequencing (in collaboration with the Eukaryotic Single Cell Genomics platform at SciLifeLab, Solna) 
  • Cell sorting and Flow cytometry (in collaboration with Biomedicum Flow Cytometry Core)
  • Various methods for pathogen inactivation and equipment decontamination, including hydrogen-peroxide vapor decontamination
  • Developing new methods in BSL3
  • Helping users with experiments 


Support, collaborations and other initiatives

BSL3 Biomedicum receives core-facility funding from Karolinska Institutet. The facility collaborates  with SciLifeLab platforms in Solna and is open to partnerships in academia, healthcare and the private sector. 

BSL3 Biomedicum  is leading a Pandemic Laboratory Preparedness initiative for Swedish BSL3 laboratories, read more here.