Automatic electrophoresis device – BioClinicum J6:30
The OnkPat/Theme Cancer core facilities are available for staff at the Department of Oncology-Pathology in addition to research Theme Cancer in BioClinicum. New users need an introduction to get authorized. Contact information to responsible persons can be found below. Before using this core facility send your certificate from KI laboratory safety introduction to Paula Mannström. Access to the building is also required.
TapeStation 4150 / Agilent Technologies
Room nr: U230 06 3700
Responsible person: Christian Sommerauer
General information
The TapeStation is an automatic electrophoresis device for RNA and DNA quality control, capable of running 1-16 samples at a given time. This is important for investigating RNA integrity after RNA isolation or sequencing library quality control.
The TapeStation has different ScreenTape kits for different size ranges (e.g., 1000bp / 5000bp), and detection sensitivities, the lowest being able to detect as little as 50 pg, while only requiring 1-2uL input material.
The run time is short and scales with the number of samples profiled, a single sample requiring 1-2 min, and 16 samples approximately 20min.
New users should contact the responsible person to receive an introduction and in case of problems or questions.