Histocore - our offer
A histological and immunohistochemical facility located in Biomedicum, offering competences and equipment for histology, immunohistochemistry, and RNAscope. We routinely perform cryo- and paraffin sectioning of tissues of animal and human origin. We also assist in conceptualization, setting up and optimizing histological and immunohistochemical approaches specific for each research project.
We work with researchers to develop new staining and labelling approaches, based on personal needs. Our main goal is to combine the classic histological approaches with cutting-edge technology like RNA in situ using RNAscope technology.
Main services
- Introduction and training for self-use of the equipment
- Consultation
- Tissue dehydration
- Embedding (organoids and tissues)
- Cryosectioning
- Paraffin sectioning
- Histological staining (HE, Oil red O, Massons Trichrome, Sirius red, Alcian Blue etc.)
- Immunohistochemistry, Multiplex Immunofluorescence
- RNAscope
Consultations
All researchers can contact us for consultancy on:
- experimental design and planning
- establishment and optimization of protocols
- project-relevant tissue processing (dehydration, cryopreservation, tissue-clearing etc.)
- histological staining and related troubleshooting
- sectioning frozen and paraffin embedded speciments and related troubleshooting
- immunostaining using fluorescent or chromogenic detection and related troubleshooting
- RNAscope
Trainings
We provide:
- 1,5-hour training for self-use of cryostats, microtomes and paraffin embedding station.
- Vibratome training from 30 minutes upt to 1 hour, depending on customers' needs.
Please send request for training in iLab. Your training will be scheduled within one to two weeks from submission of your request.
Please note that tissue dehydration is performed only by staff, and no training is provided for this.
Available instruments
- Cryostats (CryoStar NX70) (2)
- Cryostats (LEICA CM3050S) (2)
- Microtomes (HM 355S & HM360)
- Vibratome (Leica VT100ST) (2)
- Paraffin embedding station – Cryo Console EC500-1
- Dehydration machine - Epredia Excelsior AS tissue processor
- Staining platform
- Light microscope
- RNAscope HybEZ oven
- 3D printer
Rates (2024/2025)
Training
Cryo & paraffin
- Internal (KI): 1,500 SEK
- External academia: 2,000 SEK
- Corporate: 2,500 SEK
Frozen sections
Price per slide with two serial sections
If specific demand for tissue orientation/slide composition, price is calculated per hour of service. If less than 30 sections, price is calculated per hour of service.
- Internal (KI): 60 SEK
- External academia: 75 SEK
- Corporate: 95 SEK
Paraffin sections
Price per slide with two serial sections
If specific demand for tissue orientation/slide composition, price is calculated per hour of service. If less than 30 sections, price is calculated per hour of service.
- Internal (KI): 45 SEK
- External academia: 60 SEK
- Corporate: 75 SEK
Dehydration
Standard programme run on Wednesdays, charged per sample.
- Internal (KI): 34 SEK
- External academia: 46 SEK
- Corporate: 58 SEK
Services charged per hour
Consultations, embedding, staining, imaging, sample preparation, special demands, RNAscope.
- Internal (KI): 1,200 SEK
- External academia: 1,500 SEK
- Corporate: 1,900 SEK
iLab
For KI users
All our services, as well as equipment bookings, are managed through iLab. If you wish to file a request and belong to KI, please log in with your KI credentials, search for Histological Core Facility in Biomedicum and choose “Request Services”.
External use of the core facility
Histocore can services without a commissioned/contract research or collaborative research agreement, in accordance with the Swedish governmental ordinance (2022:1378) on fees for research infrastructure.
Find out more about external use of KI's core facilities
Please note that external users will need to create an iLab account to be able to send requests or book equipment.