PSF general information and operating guidelines
The aim of PSF is to provide cloning, expression screening and protein production services as well as access to instrumentation for high throughput crystallography and biophysical characterization to facilitate research activities for academic research groups. The PSF platform is built on the experiences from the Structural Genomics Consortium and is supported by Karolinska Institutet, VR-Swedstruct and SciLifeLab.
How to become a user
After an initial contact we arrange a meeting to discuss the details and timing of a project. For the national services an application form is then to be filled in to assist the SWEDSTRUCT board, appointed by VR, to prioritize projects based on scientific impact if required due to overfilled capacity. We try to accommodate requests as soon as possible and generally we can start to work on a project within a few days after agreement.
Cloning and protein production, general enquiries
User fees and billing
The user fees for Swedish academics are based on cost for consumables, instruments, university OH, and part of the cost for staff and rent. Commercial users pay full costs including staff, premises and an added margin. Examples of Swedish academic user fees are listed below, for other services please contact us:
|User training charges||Academic Price||per Item|
|Phoenix, Mosquito and RockMaker/Imager/Web||3000 kr||per person and event|
|Tecan and more RockMaker||3000 kr||per person and event|
|ITC-200||1500 kr||per person and event|
|Biacore 2000||1500 kr||per person and event|
|Self-service charges||Academic Price||per Item||Items included|
|Phoenix, Mosquito, Imaging||650 kr||per plate||Mosquito Tips, Strip & BarCode, Plate, ClearSeal, Tape, Screen, Additive HT & imaging|
|Swiss-LCP||750 kr||per plate||Mosquito Tips, BarCode, Screen, Swiss-LCP plate, 96 clear TTP plate & imaging|
|Personal user fee||1500 kr||per person and quarter||Remote data collection setup, beamtime admin, pucks, vials, dewars, freezing equipment etc|
|ITC-200||350 kr||per hour||none|
|CD||250 kr||per hour||Nitrogen gas|
|BioRad CFX (Thermofluor)||300 kr||per hour||sypro-orange, plates, seal film and plastics|
|Stargazer (thermal aggregation)||400 kr||per hour||plates and plastics|
|Biacore 2000||300 kr||per hour||plastic vials, reagents|
|Service charges||Academic Price||per Item||Description and material returned to customer|
|Escherichia coli 1.5 l culture||713 kr||1.5 liter culture||Overnight culture including induction and harvesting => Frozen pellet of cells|
|Protein 2-step purification||2888 kr||2-step purification||IMAC-SEC on ÄKTA Xpress, SDS PAGE, concentration, aliquotation, liquid nitrogen flash freeze => Purified protein and PDF documentation|
|Tag removal (TEV cleavage) plus re-purification||2505 kr||protein batch||TEV cleavage, SDS PAGE, IMAC, SDS PAGE, MS, concentration => Purified protein and PDF documentation|
All experimental work is included in the invoices for protein production services. Projects/samples that fail during technically successful experiments are transferred back to the user together with generated data. Additional investigation or problem solving is performed after discussion and agreement with the user.
Instrument access is granted on fulfilling the user training and charged by user recording actual instrument use in log-books. Recurring failures to do so will result in a penalty fee and instrument access being revoked.
Invoice specifications are emailed to the user/PI quarterly and followed by an invoice sent by KI’s central economy service.
How to acknowledge PSF
To support PSF in future evaluations and applications all users are required to acknowledge experimentation or services performed at PSF in manuscripts submitted for publication. We are grateful for information about publications where PSF have been of use.
Part of this work was facilitated by the Protein Science Facility at Karolinska Institutet/SciLifeLab (http://ki.se/psf)
Part of this work was performed at the Karolinska Institutet/SciLifeLab Protein Science Core Facility (http://ki.se/psf)